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Biosensors for Food Analysis: Development and Applications

Nistor, Mihaela LU (2004)
Abstract
Reliable analytical methods are increasingly needed in the food and beverage industry for the determination of specific components (e.g. sugars, proteins, vitamins, fats) and for detecting and quantifying chemical contaminants such as pesticides, heavy metals, antibiotics, and pathogenic microorganisms. The need arises from numerous regulatory actions and increased consumer concern about food composition and safety. The producers conventionally utilize analytical techniques such as spectrophotometry and chromatography, which do not always allow easy and continuous monitoring, are rather expensive, need well trained operators and often require sample pre-treatment steps, resulting in increased time and low cost-effectiveness of the... (More)
Reliable analytical methods are increasingly needed in the food and beverage industry for the determination of specific components (e.g. sugars, proteins, vitamins, fats) and for detecting and quantifying chemical contaminants such as pesticides, heavy metals, antibiotics, and pathogenic microorganisms. The need arises from numerous regulatory actions and increased consumer concern about food composition and safety. The producers conventionally utilize analytical techniques such as spectrophotometry and chromatography, which do not always allow easy and continuous monitoring, are rather expensive, need well trained operators and often require sample pre-treatment steps, resulting in increased time and low cost-effectiveness of the analysis. Biosensors offer a promising alternative to the classical analytical methods. Besides their good selectivity and low cost, they can easily be integrated in automation schemes and used to develop simple and even portable equipment, allowing fast in-situ monitoring of raw materials and food processing steps.The aim of this thesis is to review the current situation and future possibilities for the development and applications of biosensors in the food and beverage industry. Focus is placed on particular aspects related to elucidation of the working mechanisms of biosensing systems, optimisation of operating parameters, miniaturization, automation, and determination of food constituents in real samples. (Less)
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author
supervisor
opponent
  • Professor Dominguez, Elena, Department of Analytical Chemistry, Faculty of Pharmacy, University of Alcala, Madrid, Spain
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Bioteknik, Food and drink technology, Analytisk kemi, Analytical chemistry, on-line monitoring, flow injection, enzyme, amperometric detection, Biosensor, electrode, Livsmedelsteknik, Biotechnology
pages
150 pages
publisher
Mihaela Nistor, Biotechnology Department, LTH,
defense location
Hall B, Center for Chemistry and Chemical Engineering, Lund Institute of Technology
defense date
2004-11-25 10:30:00
external identifiers
  • other:ISRN: LUTKDH/TKBT-04/1077-SE
ISBN
91-89627-24-5
language
English
LU publication?
yes
additional info
Article: I. Electrooxidation mechanism of biogenic amines at amine oxidase modified graphite electrodeM. Niculescu, T. Ruzgas, C. Nistor, I. Frébort, M. Šebela, P. Peè, E. CsöregiAnalytical Chemistry, 72 (2000) 5988-5993. Article: II. Redox hydrogel-based amperometric bienzyme electrodes for fish freshness monitoringM. Niculescu, C. Nistor, I. Frébort, P. Peè, B. Mattiasson, E. CsöregiAnalytical Chemistry, 72 (2000) 1591-1597. Article: III. Quinohemoprotein alcohol dehydrogenase-based reagentless amperometric biosensor for ethanol monitoring during wine fermentationM. Niculescu, T. Erichsen, V. Sukharev, Z. Kerényi, E. Csöregi, W. SchuhmannAnaytica Chimica Acta, 463 (2002) 39-51. Article: IV. Simultaneous detection of ethanol, glucose and glycerol in wines using pyrroloquinoline quinone-dependent dehydrogenases based biosensorsM. Niculescu, R. Mieliauskiene, V. Laurinavicius, E. CsöregiFood Chemistry, 82 (2003) 481-489. Article: V. Visualization of micropatterned complex biosensor sensing chemistries by means of scanning electrochemical microscopyM. Niculescu, S. Gáspár, A. Schulte, E. Csöregi, W. SchuhmannBiosensors & Bioelectronics, 19 (2004) 1175-1184. Article: VI. Glycerol dehydrogenase based amperometric biosensor for monitoring of glycerol in alcoholic beveragesM. Niculescu, S. Sigina, E. CsöregiAnalytical Letters, 36 (2003) 1721-1737. Article: VII. Amperometric determination of acetate with a tri-enzyme based sensorM. Niculescu, R. Mieliauskiene, V. Laurinavicius, E. CsöregiBiosensors & Bioelectronics, submitted.
id
eedc9fd9-4570-4797-933e-e5a91f2716d1 (old id 467510)
date added to LUP
2016-04-04 11:27:51
date last changed
2018-11-21 21:05:01
@phdthesis{eedc9fd9-4570-4797-933e-e5a91f2716d1,
  abstract     = {{Reliable analytical methods are increasingly needed in the food and beverage industry for the determination of specific components (e.g. sugars, proteins, vitamins, fats) and for detecting and quantifying chemical contaminants such as pesticides, heavy metals, antibiotics, and pathogenic microorganisms. The need arises from numerous regulatory actions and increased consumer concern about food composition and safety. The producers conventionally utilize analytical techniques such as spectrophotometry and chromatography, which do not always allow easy and continuous monitoring, are rather expensive, need well trained operators and often require sample pre-treatment steps, resulting in increased time and low cost-effectiveness of the analysis. Biosensors offer a promising alternative to the classical analytical methods. Besides their good selectivity and low cost, they can easily be integrated in automation schemes and used to develop simple and even portable equipment, allowing fast in-situ monitoring of raw materials and food processing steps.The aim of this thesis is to review the current situation and future possibilities for the development and applications of biosensors in the food and beverage industry. Focus is placed on particular aspects related to elucidation of the working mechanisms of biosensing systems, optimisation of operating parameters, miniaturization, automation, and determination of food constituents in real samples.}},
  author       = {{Nistor, Mihaela}},
  isbn         = {{91-89627-24-5}},
  keywords     = {{Bioteknik; Food and drink technology; Analytisk kemi; Analytical chemistry; on-line monitoring; flow injection; enzyme; amperometric detection; Biosensor; electrode; Livsmedelsteknik; Biotechnology}},
  language     = {{eng}},
  publisher    = {{Mihaela Nistor, Biotechnology Department, LTH,}},
  school       = {{Lund University}},
  title        = {{Biosensors for Food Analysis: Development and Applications}},
  year         = {{2004}},
}