A pilot study investigating lactic acid bacterial symbionts from the honeybee in inhibiting human chronic wound pathogens.
Butler, Éile LU ; Öien, Rut LU ; Lindholm, Christina ; Olofsson, Tobias LU ; Nilson, Bo LU
and Vasquez, Alejandra
LU
(2016)
In International Wound Journal
13(5).
p.729-737
- Abstract
- Treatment and management of chronic wounds is a large burden on the health sector and causes substantial suffering for the patients. We believe that 13 lactic acid bacteria (LAB) symbionts isolated from the honey crop of the honeybee are important players in the antimicrobial action of honey, by producing antimicrobial substances and can be used in combination with heather honey as an effective treatment in wound management. A total of 22 patients with chronic ulcers were included; culture-dependent and molecular-based (MALDI-MS and 16S rRNA gene sequencing) techniques were used to identify bacteria from chronic wounds. These clinical isolates were used for in vitro antimicrobial testing with standardised viable LAB and sterilised heather... (More)
- Treatment and management of chronic wounds is a large burden on the health sector and causes substantial suffering for the patients. We believe that 13 lactic acid bacteria (LAB) symbionts isolated from the honey crop of the honeybee are important players in the antimicrobial action of honey, by producing antimicrobial substances and can be used in combination with heather honey as an effective treatment in wound management. A total of 22 patients with chronic ulcers were included; culture-dependent and molecular-based (MALDI-MS and 16S rRNA gene sequencing) techniques were used to identify bacteria from chronic wounds. These clinical isolates were used for in vitro antimicrobial testing with standardised viable LAB and sterilised heather honey mixture. Twenty of the patients' wounds were polymicrobial and 42 different species were isolated. Patient isolates that were tested in vitro were inhibited by the LAB and honey combination with inhibitory zones comparable with different antibiotics. LAB and heather honey in combination presents a new topical option in chronic wound management because of the healing properties of honey, antimicrobial metabolite production from the LAB and their bactericidal effect on common chronic wound pathogens. This new treatment may be a stepping stone towards an alternative solution to antibiotics. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4692099
- author
- Butler, Éile
LU
; Öien, Rut
LU
; Lindholm, Christina
; Olofsson, Tobias
LU
; Nilson, Bo
LU
and Vasquez, Alejandra
LU
- organization
- publishing date
- 2016
- type
- Contribution to journal
- publication status
- published
- subject
- in
- International Wound Journal
- volume
- 13
- issue
- 5
- pages
- 729 - 737
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:25196349
- pmid:25196349
- wos:000387667600073
- scopus:85021346400
- ISSN
- 1742-481X
- DOI
- 10.1111/iwj.12360
- language
- English
- LU publication?
- yes
- id
- c09d8fc1-1c46-4d4f-877d-1a3797d58d85 (old id 4692099)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/25196349?dopt=Abstract
- date added to LUP
- 2016-04-04 07:24:56
- date last changed
- 2023-04-08 23:12:16
@article{c09d8fc1-1c46-4d4f-877d-1a3797d58d85,
abstract = {{Treatment and management of chronic wounds is a large burden on the health sector and causes substantial suffering for the patients. We believe that 13 lactic acid bacteria (LAB) symbionts isolated from the honey crop of the honeybee are important players in the antimicrobial action of honey, by producing antimicrobial substances and can be used in combination with heather honey as an effective treatment in wound management. A total of 22 patients with chronic ulcers were included; culture-dependent and molecular-based (MALDI-MS and 16S rRNA gene sequencing) techniques were used to identify bacteria from chronic wounds. These clinical isolates were used for in vitro antimicrobial testing with standardised viable LAB and sterilised heather honey mixture. Twenty of the patients' wounds were polymicrobial and 42 different species were isolated. Patient isolates that were tested in vitro were inhibited by the LAB and honey combination with inhibitory zones comparable with different antibiotics. LAB and heather honey in combination presents a new topical option in chronic wound management because of the healing properties of honey, antimicrobial metabolite production from the LAB and their bactericidal effect on common chronic wound pathogens. This new treatment may be a stepping stone towards an alternative solution to antibiotics.}},
author = {{Butler, Éile and Öien, Rut and Lindholm, Christina and Olofsson, Tobias and Nilson, Bo and Vasquez, Alejandra}},
issn = {{1742-481X}},
language = {{eng}},
number = {{5}},
pages = {{729--737}},
publisher = {{Wiley-Blackwell}},
series = {{International Wound Journal}},
title = {{A pilot study investigating lactic acid bacterial symbionts from the honeybee in inhibiting human chronic wound pathogens.}},
url = {{http://dx.doi.org/10.1111/iwj.12360}},
doi = {{10.1111/iwj.12360}},
volume = {{13}},
year = {{2016}},
}