Construction and Characterization of Kilobasepair Densely Labeled Peptide-DNA
Kovacic, Suzana ; Samii, Laleh ; Lamour, Guillaume ; Li, Hongbin ; Linke, Heiner LU
; Bromley, Elizabeth H. C.
; Woolfson, Derek N.
; Curmi, Paul M. G.
and Forde, Nancy R.
(2014)
In Biomacromolecules
15(11).
p.4065-4072
- Abstract
- Directed assembly of biocompatible materials benefits from modular building blocks in which structural organization is independent of introduced functional modifications. For soft materials, such modifications have been limited. Here, long DNA is successfully functionalized with dense decoration by peptides. Following introduction of alkyne-modified nucleotides into kilobasepair DNA, measurements of persistence length show that DNA mechanics are unaltered by the dense incorporation of alkynes (similar to 1 alkyne/2 bp) and after click-chemistry attachment of a tunable density of peptides. Proteolytic cleavage of densely tethered peptides (similar to 1 peptide/3 bp) demonstrates addressability of the functional groups, showing that this... (More)
- Directed assembly of biocompatible materials benefits from modular building blocks in which structural organization is independent of introduced functional modifications. For soft materials, such modifications have been limited. Here, long DNA is successfully functionalized with dense decoration by peptides. Following introduction of alkyne-modified nucleotides into kilobasepair DNA, measurements of persistence length show that DNA mechanics are unaltered by the dense incorporation of alkynes (similar to 1 alkyne/2 bp) and after click-chemistry attachment of a tunable density of peptides. Proteolytic cleavage of densely tethered peptides (similar to 1 peptide/3 bp) demonstrates addressability of the functional groups, showing that this accessible approach to creating hybrid structures can maintain orthogonality between backbone mechanics and overlaid function. The synthesis and characterization of these hybrid constructs establishes the groundwork for their implementation in future applications, such as building blocks in modular approaches to a range of problems in synthetic biology. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4865268
- author
- Kovacic, Suzana
; Samii, Laleh
; Lamour, Guillaume
; Li, Hongbin
; Linke, Heiner
LU
; Bromley, Elizabeth H. C.
; Woolfson, Derek N.
; Curmi, Paul M. G.
and Forde, Nancy R.
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biomacromolecules
- volume
- 15
- issue
- 11
- pages
- 4065 - 4072
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000344721400020
- scopus:84910069461
- ISSN
- 1526-4602
- DOI
- 10.1021/bm501109p
- language
- English
- LU publication?
- yes
- id
- 01aa8e3f-e2ca-495d-9205-c18d56a939ec (old id 4865268)
- date added to LUP
- 2016-04-01 10:25:45
- date last changed
- 2023-10-26 10:11:57
@article{01aa8e3f-e2ca-495d-9205-c18d56a939ec,
abstract = {{Directed assembly of biocompatible materials benefits from modular building blocks in which structural organization is independent of introduced functional modifications. For soft materials, such modifications have been limited. Here, long DNA is successfully functionalized with dense decoration by peptides. Following introduction of alkyne-modified nucleotides into kilobasepair DNA, measurements of persistence length show that DNA mechanics are unaltered by the dense incorporation of alkynes (similar to 1 alkyne/2 bp) and after click-chemistry attachment of a tunable density of peptides. Proteolytic cleavage of densely tethered peptides (similar to 1 peptide/3 bp) demonstrates addressability of the functional groups, showing that this accessible approach to creating hybrid structures can maintain orthogonality between backbone mechanics and overlaid function. The synthesis and characterization of these hybrid constructs establishes the groundwork for their implementation in future applications, such as building blocks in modular approaches to a range of problems in synthetic biology.}},
author = {{Kovacic, Suzana and Samii, Laleh and Lamour, Guillaume and Li, Hongbin and Linke, Heiner and Bromley, Elizabeth H. C. and Woolfson, Derek N. and Curmi, Paul M. G. and Forde, Nancy R.}},
issn = {{1526-4602}},
language = {{eng}},
number = {{11}},
pages = {{4065--4072}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Biomacromolecules}},
title = {{Construction and Characterization of Kilobasepair Densely Labeled Peptide-DNA}},
url = {{http://dx.doi.org/10.1021/bm501109p}},
doi = {{10.1021/bm501109p}},
volume = {{15}},
year = {{2014}},
}