Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Structural effects of the dispersing agent polysorbate 80 on liquid crystalline nanoparticles of soy phosphatidylcholine and glycerol dioleate.

Wadsäter, Maria LU ; Barauskas, Justas ; Rogers, Sarah ; Skoda, Maximilian W A ; Thomas, Robert K ; Tiberg, Fredrik LU and Nylander, Tommy LU (2015) In Soft Matter 11(6). p.1140-1150
Abstract
Well-defined, stable and highly structured I2 (Fd3[combining macron]m) liquid crystalline nanoparticles (LCNP) of 50/50 (wt/wt) soy phosphatidylcholine (SPC)/glycerol dioleate (GDO), can be formed by using a low fraction (5-10 wt%) of the dispersing polymeric surfactant polyoxyethylene (20) sorbitan monooleate (polysorbate 80 or P80). In the present study we used small angle neutron scattering (SANS) and deuterated P80 (d-P80) to determine the location and concentration of P80 within the LCNP and small angle X-ray scattering (SAXS) to reveal the internal structure. SANS data suggests that some d-P80 already penetrates the particle core at 5%. However, the content of d-P80 is still low enough not to significantly change the internal... (More)
Well-defined, stable and highly structured I2 (Fd3[combining macron]m) liquid crystalline nanoparticles (LCNP) of 50/50 (wt/wt) soy phosphatidylcholine (SPC)/glycerol dioleate (GDO), can be formed by using a low fraction (5-10 wt%) of the dispersing polymeric surfactant polyoxyethylene (20) sorbitan monooleate (polysorbate 80 or P80). In the present study we used small angle neutron scattering (SANS) and deuterated P80 (d-P80) to determine the location and concentration of P80 within the LCNP and small angle X-ray scattering (SAXS) to reveal the internal structure. SANS data suggests that some d-P80 already penetrates the particle core at 5%. However, the content of d-P80 is still low enough not to significantly change the internal Fd3[combining macron]m structure of the LCNP. At higher fractions of P80 a phase separation occurs, in which a SPC and P80 rich phase is formed at the particle surface. The surface layer becomes gradually richer in both solvent and d-P80 when the surfactant concentration is increased from 5 to 15%, while the core of the particle is enriched by GDO, resulting in loss of internal structure and reduced hydration. We have used neutron reflectometry to reveal the location of the stabiliser within the adsorbed layer on an anionic silica and cationic (aminopropyltriethoxysilane (APTES) silanized) surface. d-P80 is enriched closest to the supporting surface and slightly more so for the cationic APTES surface. The results are relevant not only for the capability of LCNPs as drug delivery vehicles but also as means of preparing functional surface coatings. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Soft Matter
volume
11
issue
6
pages
1140 - 1150
publisher
Royal Society of Chemistry
external identifiers
  • pmid:25531822
  • wos:000349146400012
  • scopus:84964255283
  • pmid:25531822
ISSN
1744-6848
DOI
10.1039/c4sm02296c
language
English
LU publication?
yes
id
07f3ee91-0562-47db-b61f-43abd063e27a (old id 4905784)
date added to LUP
2016-04-01 11:14:22
date last changed
2023-10-13 00:09:28
@article{07f3ee91-0562-47db-b61f-43abd063e27a,
  abstract     = {{Well-defined, stable and highly structured I2 (Fd3[combining macron]m) liquid crystalline nanoparticles (LCNP) of 50/50 (wt/wt) soy phosphatidylcholine (SPC)/glycerol dioleate (GDO), can be formed by using a low fraction (5-10 wt%) of the dispersing polymeric surfactant polyoxyethylene (20) sorbitan monooleate (polysorbate 80 or P80). In the present study we used small angle neutron scattering (SANS) and deuterated P80 (d-P80) to determine the location and concentration of P80 within the LCNP and small angle X-ray scattering (SAXS) to reveal the internal structure. SANS data suggests that some d-P80 already penetrates the particle core at 5%. However, the content of d-P80 is still low enough not to significantly change the internal Fd3[combining macron]m structure of the LCNP. At higher fractions of P80 a phase separation occurs, in which a SPC and P80 rich phase is formed at the particle surface. The surface layer becomes gradually richer in both solvent and d-P80 when the surfactant concentration is increased from 5 to 15%, while the core of the particle is enriched by GDO, resulting in loss of internal structure and reduced hydration. We have used neutron reflectometry to reveal the location of the stabiliser within the adsorbed layer on an anionic silica and cationic (aminopropyltriethoxysilane (APTES) silanized) surface. d-P80 is enriched closest to the supporting surface and slightly more so for the cationic APTES surface. The results are relevant not only for the capability of LCNPs as drug delivery vehicles but also as means of preparing functional surface coatings.}},
  author       = {{Wadsäter, Maria and Barauskas, Justas and Rogers, Sarah and Skoda, Maximilian W A and Thomas, Robert K and Tiberg, Fredrik and Nylander, Tommy}},
  issn         = {{1744-6848}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{1140--1150}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{Soft Matter}},
  title        = {{Structural effects of the dispersing agent polysorbate 80 on liquid crystalline nanoparticles of soy phosphatidylcholine and glycerol dioleate.}},
  url          = {{http://dx.doi.org/10.1039/c4sm02296c}},
  doi          = {{10.1039/c4sm02296c}},
  volume       = {{11}},
  year         = {{2015}},
}