Development of Plasmodium falciparum specific naïve, atypical, memory and plasma B cells during infancy and in adults in an endemic area
(2017) In Malaria Journal 16(1).- Abstract
Background: B-cells are essential in immunity against malaria, but which sub-sets of B-cells specifically recognize Plasmodium falciparum and when they appear is still largely unknown. Results: Using the flow cytometry technique for detection of P. falciparum specific (Pf+) B-cells, this study for the first time measured the development of Pf+ B cell (CD19+) phenotypes in Ugandan babies from birth up to nine months, and in their mothers. The babies showed increases in Pf+ IgG memory B-cells (MBCs), atypical MBCs, and plasma cells/blasts over time, but the proportion of these cells were still lower than in the mothers who displayed stable levels (5, 18, and 3%, respectively). Pf+ non-IgG+ MBCs and naïve B-cells binding to P. falciparum... (More)
Background: B-cells are essential in immunity against malaria, but which sub-sets of B-cells specifically recognize Plasmodium falciparum and when they appear is still largely unknown. Results: Using the flow cytometry technique for detection of P. falciparum specific (Pf+) B-cells, this study for the first time measured the development of Pf+ B cell (CD19+) phenotypes in Ugandan babies from birth up to nine months, and in their mothers. The babies showed increases in Pf+ IgG memory B-cells (MBCs), atypical MBCs, and plasma cells/blasts over time, but the proportion of these cells were still lower than in the mothers who displayed stable levels (5, 18, and 3%, respectively). Pf+ non-IgG+ MBCs and naïve B-cells binding to P. falciparum antigens were higher in the babies compared to the mothers (12 and 50%). In ELISA there was an increase in IgG and IgM antibodies over time in babies, and stable levels in mothers. At baby delivery, multigravidae mothers had a higher proportion of Pf+ IgG MBCs and less Pf+ naïve B-cells than primigravidae mothers. Conclusions: In newborns, naïve B-cells are a major player in recognizing P. falciparum. In adults, the high proportion of Pf+ atypical MBCs suggests a major role for these cells. Both in infants and adults, non-IgG+ MBCs were higher than IgG MBCs, indicating that these cells deserve more focus in future.
(Less)
- author
- Lugaajju, Allan ; Reddy, Sreenivasulu B. ; Wahlgren, Mats ; Kironde, Fred and Persson, Kristina E M LU
- organization
- publishing date
- 2017-01-21
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Atypical, B-cells, Immunity, Malaria, Memory, Plasmodium falciparum
- in
- Malaria Journal
- volume
- 16
- issue
- 1
- article number
- 37
- publisher
- BioMed Central (BMC)
- external identifiers
-
- pmid:28109284
- wos:000392761400001
- scopus:85009945399
- ISSN
- 1475-2875
- DOI
- 10.1186/s12936-017-1697-z
- language
- English
- LU publication?
- yes
- id
- 4d20ac60-0b66-44e7-b3d2-82b8385dbf29
- date added to LUP
- 2017-02-03 15:28:14
- date last changed
- 2024-04-16 09:04:57
@article{4d20ac60-0b66-44e7-b3d2-82b8385dbf29,
abstract = {{<p>Background: B-cells are essential in immunity against malaria, but which sub-sets of B-cells specifically recognize Plasmodium falciparum and when they appear is still largely unknown. Results: Using the flow cytometry technique for detection of P. falciparum specific (Pf+) B-cells, this study for the first time measured the development of Pf+ B cell (CD19+) phenotypes in Ugandan babies from birth up to nine months, and in their mothers. The babies showed increases in Pf+ IgG memory B-cells (MBCs), atypical MBCs, and plasma cells/blasts over time, but the proportion of these cells were still lower than in the mothers who displayed stable levels (5, 18, and 3%, respectively). Pf+ non-IgG+ MBCs and naïve B-cells binding to P. falciparum antigens were higher in the babies compared to the mothers (12 and 50%). In ELISA there was an increase in IgG and IgM antibodies over time in babies, and stable levels in mothers. At baby delivery, multigravidae mothers had a higher proportion of Pf+ IgG MBCs and less Pf+ naïve B-cells than primigravidae mothers. Conclusions: In newborns, naïve B-cells are a major player in recognizing P. falciparum. In adults, the high proportion of Pf+ atypical MBCs suggests a major role for these cells. Both in infants and adults, non-IgG+ MBCs were higher than IgG MBCs, indicating that these cells deserve more focus in future.</p>}},
author = {{Lugaajju, Allan and Reddy, Sreenivasulu B. and Wahlgren, Mats and Kironde, Fred and Persson, Kristina E M}},
issn = {{1475-2875}},
keywords = {{Atypical; B-cells; Immunity; Malaria; Memory; Plasmodium falciparum}},
language = {{eng}},
month = {{01}},
number = {{1}},
publisher = {{BioMed Central (BMC)}},
series = {{Malaria Journal}},
title = {{Development of Plasmodium falciparum specific naïve, atypical, memory and plasma B cells during infancy and in adults in an endemic area}},
url = {{http://dx.doi.org/10.1186/s12936-017-1697-z}},
doi = {{10.1186/s12936-017-1697-z}},
volume = {{16}},
year = {{2017}},
}