The dynamics of insulin release from mouse pancreatic islet cells in suspension
Idahl, Lars åke ; Lernmark, Åke LU
; Sehlin, Janove
and Täljedal, Inge Bert
(1976)
In Pflügers Archiv
366(2-3).
p.185-188
- Abstract
The overall dynamics of glucose-induced insulin release was strikingly similar in dispersed cells and intact islets perifused in parallel. Both preparations exhibited a latency of 1-2 min, after which period there was a brisk rise of insulin release followed by a sustained second phase. During the second phase, insulin release from dispersed cells attained a stable plateau rate, whereas the release from intact islets continued to rise. Epinephrine (1 μM) inhibited the release in both preparations, but the return to basal rate was faster in the dispersed cells than in the intact islets. The dispersed cells oxidized glucose at a constant rate for at least 60 min; the glucose oxidation was markedly sensitive to changes of the glucose... (More)
The overall dynamics of glucose-induced insulin release was strikingly similar in dispersed cells and intact islets perifused in parallel. Both preparations exhibited a latency of 1-2 min, after which period there was a brisk rise of insulin release followed by a sustained second phase. During the second phase, insulin release from dispersed cells attained a stable plateau rate, whereas the release from intact islets continued to rise. Epinephrine (1 μM) inhibited the release in both preparations, but the return to basal rate was faster in the dispersed cells than in the intact islets. The dispersed cells oxidized glucose at a constant rate for at least 60 min; the glucose oxidation was markedly sensitive to changes of the glucose concentration in the range of 3-20 mM.
(Less)
- author
- Idahl, Lars åke
; Lernmark, Åke
LU
; Sehlin, Janove
and Täljedal, Inge Bert
- publishing date
- 1976-01-01
- type
- Contribution to journal
- publication status
- published
- keywords
- β-Cells in suspension, Glucose oxidation, Insulin release, Intact pancreatic islets, Pancreatic β-cells
- in
- Pflügers Archiv
- volume
- 366
- issue
- 2-3
- pages
- 185 - 188
- publisher
- Springer
- external identifiers
-
- pmid:186754
- scopus:0017097090
- ISSN
- 0031-6768
- DOI
- 10.1007/BF00585876
- language
- English
- LU publication?
- no
- id
- 517e83ee-3431-45b9-a83a-8c4ac48f64d2
- date added to LUP
- 2019-09-18 12:12:58
- date last changed
- 2024-03-13 08:03:05
@article{517e83ee-3431-45b9-a83a-8c4ac48f64d2,
abstract = {{<p>The overall dynamics of glucose-induced insulin release was strikingly similar in dispersed cells and intact islets perifused in parallel. Both preparations exhibited a latency of 1-2 min, after which period there was a brisk rise of insulin release followed by a sustained second phase. During the second phase, insulin release from dispersed cells attained a stable plateau rate, whereas the release from intact islets continued to rise. Epinephrine (1 μM) inhibited the release in both preparations, but the return to basal rate was faster in the dispersed cells than in the intact islets. The dispersed cells oxidized glucose at a constant rate for at least 60 min; the glucose oxidation was markedly sensitive to changes of the glucose concentration in the range of 3-20 mM.</p>}},
author = {{Idahl, Lars åke and Lernmark, Åke and Sehlin, Janove and Täljedal, Inge Bert}},
issn = {{0031-6768}},
keywords = {{β-Cells in suspension; Glucose oxidation; Insulin release; Intact pancreatic islets; Pancreatic β-cells}},
language = {{eng}},
month = {{01}},
number = {{2-3}},
pages = {{185--188}},
publisher = {{Springer}},
series = {{Pflügers Archiv}},
title = {{The dynamics of insulin release from mouse pancreatic islet cells in suspension}},
url = {{http://dx.doi.org/10.1007/BF00585876}},
doi = {{10.1007/BF00585876}},
volume = {{366}},
year = {{1976}},
}