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Ion exchange chromatography of glucosamine and galactosamine in microgram amounts with quantitative determination and specific radioactivity assay

Lohmander, Stefan LU orcid (1972) In Biochimica et Biophysica Acta. General Subjects 264(3). p.411-417
Abstract

A rapid method for the separation and quantitative determination, including radioassay, of glucosamine and galactosamine in microgram amounts is described. It is based upon the use of a column of Aminex A-5 ion exchange resin eluted with a phosphate buffer at pH 7.00 and 60 °C. From each fraction half the volume is used for a scaled down Elson-Morgan procedure and other half for liquid scintillation counting. Amounts of about 0.01-1 μmole of hexosamine may be separated and determined.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochimica et Biophysica Acta. General Subjects
volume
264
issue
3
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:0015512384
  • pmid:5027625
ISSN
0304-4165
DOI
10.1016/0304-4165(72)90003-7
language
English
LU publication?
yes
id
586a4d48-6d98-4dda-aab3-ca3ab2befddc
date added to LUP
2016-05-04 12:36:47
date last changed
2024-01-04 02:33:20
@article{586a4d48-6d98-4dda-aab3-ca3ab2befddc,
  abstract     = {{<p>A rapid method for the separation and quantitative determination, including radioassay, of glucosamine and galactosamine in microgram amounts is described. It is based upon the use of a column of Aminex A-5 ion exchange resin eluted with a phosphate buffer at pH 7.00 and 60 °C. From each fraction half the volume is used for a scaled down Elson-Morgan procedure and other half for liquid scintillation counting. Amounts of about 0.01-1 μmole of hexosamine may be separated and determined.</p>}},
  author       = {{Lohmander, Stefan}},
  issn         = {{0304-4165}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{3}},
  pages        = {{411--417}},
  publisher    = {{Elsevier}},
  series       = {{Biochimica et Biophysica Acta. General Subjects}},
  title        = {{Ion exchange chromatography of glucosamine and galactosamine in microgram amounts with quantitative determination and specific radioactivity assay}},
  url          = {{http://dx.doi.org/10.1016/0304-4165(72)90003-7}},
  doi          = {{10.1016/0304-4165(72)90003-7}},
  volume       = {{264}},
  year         = {{1972}},
}