Exploring variation in binding of Protein A and Protein G to immunoglobulin type G by isothermal titration calorimetry
(2011) In Journal of Molecular Recognition 24(6). p.945-952- Abstract
- Bacterial Protein A (PrtA) and Protein G (PrtG) are widely used for affinity purification of antibodies. An understanding of how PrtA and PrtG bind to different isotypes of immunoglobulin type G (IgG) and to their corresponding Fc fragments is essential for the development of PrtA and PrtG mimetic ligands and for the establishment of generic processes for the purification of various antibodies. In this paper, the interactions between the two IgG-binding proteins and IgG of two different subclasses, IgG1 and IgG4, as well as their analogous Fc fragments have been studied by isothermal titration calorimetry. The results indicate that both protein ligands bind IgG and Fc fragments strongly with Ka values in the range of 10(7)-10(8) M(-1) and... (More)
- Bacterial Protein A (PrtA) and Protein G (PrtG) are widely used for affinity purification of antibodies. An understanding of how PrtA and PrtG bind to different isotypes of immunoglobulin type G (IgG) and to their corresponding Fc fragments is essential for the development of PrtA and PrtG mimetic ligands and for the establishment of generic processes for the purification of various antibodies. In this paper, the interactions between the two IgG-binding proteins and IgG of two different subclasses, IgG1 and IgG4, as well as their analogous Fc fragments have been studied by isothermal titration calorimetry. The results indicate that both protein ligands bind IgG and Fc fragments strongly with Ka values in the range of 10(7)-10(8) M(-1) and for both ligands, the interaction with both IgG isotypes is enthalpically driven though entropically unfavorable. Moreover, variation in the standard entropic and standard enthalpic contribution to binding between the two isotypes as well as between IgG and Fc fragment implies that the specific interaction with PrtA varies according to IgG isotype. In contrast to PrtA, PrtG bound to F(ab')(2) fragment with a Ka value of 5.1 x 10(5) M(-1); thus underscoring the usefulness of PrtA as a preferred ligand for generic antibody purification processes. Copyright (C) 2011 John Wiley & Sons, Ltd. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2313127
- author
- Lund, Line Naomi ; Christensen, Trine ; Toone, Eric ; Houen, Gunnar ; Staby, Arne LU and St Hilaire, Phaedria Marie
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Protein A, Protein G, immunoglobulin G, Fc fragment, isothermal, titration calorimetry
- in
- Journal of Molecular Recognition
- volume
- 24
- issue
- 6
- pages
- 945 - 952
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000298599500006
- scopus:80155178893
- pmid:22038801
- ISSN
- 1099-1352
- DOI
- 10.1002/jmr.1140
- language
- English
- LU publication?
- yes
- id
- 5b72eebe-f070-4b06-b2de-fa0deed4c4c3 (old id 2313127)
- date added to LUP
- 2016-04-01 10:15:00
- date last changed
- 2023-12-08 13:49:57
@article{5b72eebe-f070-4b06-b2de-fa0deed4c4c3,
abstract = {{Bacterial Protein A (PrtA) and Protein G (PrtG) are widely used for affinity purification of antibodies. An understanding of how PrtA and PrtG bind to different isotypes of immunoglobulin type G (IgG) and to their corresponding Fc fragments is essential for the development of PrtA and PrtG mimetic ligands and for the establishment of generic processes for the purification of various antibodies. In this paper, the interactions between the two IgG-binding proteins and IgG of two different subclasses, IgG1 and IgG4, as well as their analogous Fc fragments have been studied by isothermal titration calorimetry. The results indicate that both protein ligands bind IgG and Fc fragments strongly with Ka values in the range of 10(7)-10(8) M(-1) and for both ligands, the interaction with both IgG isotypes is enthalpically driven though entropically unfavorable. Moreover, variation in the standard entropic and standard enthalpic contribution to binding between the two isotypes as well as between IgG and Fc fragment implies that the specific interaction with PrtA varies according to IgG isotype. In contrast to PrtA, PrtG bound to F(ab')(2) fragment with a Ka value of 5.1 x 10(5) M(-1); thus underscoring the usefulness of PrtA as a preferred ligand for generic antibody purification processes. Copyright (C) 2011 John Wiley & Sons, Ltd.}},
author = {{Lund, Line Naomi and Christensen, Trine and Toone, Eric and Houen, Gunnar and Staby, Arne and St Hilaire, Phaedria Marie}},
issn = {{1099-1352}},
keywords = {{Protein A; Protein G; immunoglobulin G; Fc fragment; isothermal; titration calorimetry}},
language = {{eng}},
number = {{6}},
pages = {{945--952}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Journal of Molecular Recognition}},
title = {{Exploring variation in binding of Protein A and Protein G to immunoglobulin type G by isothermal titration calorimetry}},
url = {{http://dx.doi.org/10.1002/jmr.1140}},
doi = {{10.1002/jmr.1140}},
volume = {{24}},
year = {{2011}},
}