Estimating the total rate of DNA replication using branching processes
(2008) In Bulletin of Mathematical Biology 70(8). p.2177-2194- Abstract
- Increasing the knowledge of various cell cycle kinetic parameters, such as the length of the cell cycle and its different phases, is of considerable importance for several purposes including tumor diagnostics and treatment in clinical health care and a deepened understanding of tumor growth mechanisms. Of particular interest as a prognostic factor in different cancer forms is the S phase, during which DNA is replicated. In the present paper, we estimate the DNA replication rate and the S phase length from bromodeoxyuridine-DNA flow cytometry data. The mathematical analysis is based on a branching process model, paired with an assumed gamma distribution for the S phase duration, with which the DNA distribution of S phase cells can be... (More)
- Increasing the knowledge of various cell cycle kinetic parameters, such as the length of the cell cycle and its different phases, is of considerable importance for several purposes including tumor diagnostics and treatment in clinical health care and a deepened understanding of tumor growth mechanisms. Of particular interest as a prognostic factor in different cancer forms is the S phase, during which DNA is replicated. In the present paper, we estimate the DNA replication rate and the S phase length from bromodeoxyuridine-DNA flow cytometry data. The mathematical analysis is based on a branching process model, paired with an assumed gamma distribution for the S phase duration, with which the DNA distribution of S phase cells can be expressed in terms of the DNA replication rate. Flow cytometry data typically contains rather large measurement variations, however, and we employ nonparametric deconvolution to estimate the underlying DNA distribution of S phase cells; an estimate of the DNA replication rate is then provided by this distribution and the mathematical model. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/633097
- author
- Larsson, Sara LU ; Rydén, Tobias LU ; Holst, Ulla LU ; Johansson, Maria C LU and Oredsson, Stina LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Bulletin of Mathematical Biology
- volume
- 70
- issue
- 8
- pages
- 2177 - 2194
- publisher
- Springer
- external identifiers
-
- wos:000259962900003
- scopus:53849087811
- ISSN
- 1522-9602
- DOI
- 10.1007/s11538-008-9339-9
- language
- English
- LU publication?
- yes
- id
- aaaecefb-1d83-4664-b710-01c29df8de0d (old id 633097)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18818973?dopt=Abstract
- date added to LUP
- 2016-04-01 12:20:18
- date last changed
- 2022-03-21 02:44:55
@article{aaaecefb-1d83-4664-b710-01c29df8de0d,
abstract = {{Increasing the knowledge of various cell cycle kinetic parameters, such as the length of the cell cycle and its different phases, is of considerable importance for several purposes including tumor diagnostics and treatment in clinical health care and a deepened understanding of tumor growth mechanisms. Of particular interest as a prognostic factor in different cancer forms is the S phase, during which DNA is replicated. In the present paper, we estimate the DNA replication rate and the S phase length from bromodeoxyuridine-DNA flow cytometry data. The mathematical analysis is based on a branching process model, paired with an assumed gamma distribution for the S phase duration, with which the DNA distribution of S phase cells can be expressed in terms of the DNA replication rate. Flow cytometry data typically contains rather large measurement variations, however, and we employ nonparametric deconvolution to estimate the underlying DNA distribution of S phase cells; an estimate of the DNA replication rate is then provided by this distribution and the mathematical model.}},
author = {{Larsson, Sara and Rydén, Tobias and Holst, Ulla and Johansson, Maria C and Oredsson, Stina}},
issn = {{1522-9602}},
language = {{eng}},
number = {{8}},
pages = {{2177--2194}},
publisher = {{Springer}},
series = {{Bulletin of Mathematical Biology}},
title = {{Estimating the total rate of DNA replication using branching processes}},
url = {{http://dx.doi.org/10.1007/s11538-008-9339-9}},
doi = {{10.1007/s11538-008-9339-9}},
volume = {{70}},
year = {{2008}},
}