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L-Arabinose transport and catabolism in yeast

Fonseca, Cesar ; Romao, Rute ; de Sousa, Helena Rodrigues ; Hahn-Hägerdal, Bärbel LU and Spencer-Martins, Isabel (2007) In The FEBS Journal 274(14). p.3589-3600
Abstract
Two yeasts, Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012, which show rapid growth on L-arabinose and very high rates of L-arabinose uptake on screening, were selected for characterization of L-arabinose transport and the first steps of intracellular L-arabinose metabolism. The kinetics of L-arabinose uptake revealed at least two transport systems with distinct substrate affinities, specificities, functional mechanisms and regulatory properties. The L-arabinose catabolic pathway proposed for filamentous fungi also seems to operate in the yeasts studied. The kinetic parameters of the initial L-arabinose-metabolizing enzymes were determined. Reductases were found to be mostly NADPH-dependent, whereas NAD was the... (More)
Two yeasts, Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012, which show rapid growth on L-arabinose and very high rates of L-arabinose uptake on screening, were selected for characterization of L-arabinose transport and the first steps of intracellular L-arabinose metabolism. The kinetics of L-arabinose uptake revealed at least two transport systems with distinct substrate affinities, specificities, functional mechanisms and regulatory properties. The L-arabinose catabolic pathway proposed for filamentous fungi also seems to operate in the yeasts studied. The kinetic parameters of the initial L-arabinose-metabolizing enzymes were determined. Reductases were found to be mostly NADPH-dependent, whereas NAD was the preferred cofactor of dehydrogenases. The differences found between the two yeasts agree with the higher efficiency of L-arabinose metabolism in C. arabinofermentans. This is the first full account of the initial steps of L-arabinose catabolism in yeast including the biochemical characterization of a specific L-arabinose transporter. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
guilliermondii, Pichia, Candida arabinofermentans, L-arabinose catabolism, sugar transport, yeast
in
The FEBS Journal
volume
274
issue
14
pages
3589 - 3600
publisher
Wiley-Blackwell
external identifiers
  • wos:000247904900011
  • scopus:34447310554
ISSN
1742-464X
DOI
10.1111/j.1742-4658.2007.05892.x
language
English
LU publication?
yes
id
01c81ea9-730d-4500-9a12-c8d0a209d7ac (old id 645786)
date added to LUP
2016-04-01 16:05:50
date last changed
2022-01-28 17:17:15
@article{01c81ea9-730d-4500-9a12-c8d0a209d7ac,
  abstract     = {{Two yeasts, Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012, which show rapid growth on L-arabinose and very high rates of L-arabinose uptake on screening, were selected for characterization of L-arabinose transport and the first steps of intracellular L-arabinose metabolism. The kinetics of L-arabinose uptake revealed at least two transport systems with distinct substrate affinities, specificities, functional mechanisms and regulatory properties. The L-arabinose catabolic pathway proposed for filamentous fungi also seems to operate in the yeasts studied. The kinetic parameters of the initial L-arabinose-metabolizing enzymes were determined. Reductases were found to be mostly NADPH-dependent, whereas NAD was the preferred cofactor of dehydrogenases. The differences found between the two yeasts agree with the higher efficiency of L-arabinose metabolism in C. arabinofermentans. This is the first full account of the initial steps of L-arabinose catabolism in yeast including the biochemical characterization of a specific L-arabinose transporter.}},
  author       = {{Fonseca, Cesar and Romao, Rute and de Sousa, Helena Rodrigues and Hahn-Hägerdal, Bärbel and Spencer-Martins, Isabel}},
  issn         = {{1742-464X}},
  keywords     = {{guilliermondii; Pichia; Candida arabinofermentans; L-arabinose catabolism; sugar transport; yeast}},
  language     = {{eng}},
  number       = {{14}},
  pages        = {{3589--3600}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{The FEBS Journal}},
  title        = {{L-Arabinose transport and catabolism in yeast}},
  url          = {{http://dx.doi.org/10.1111/j.1742-4658.2007.05892.x}},
  doi          = {{10.1111/j.1742-4658.2007.05892.x}},
  volume       = {{274}},
  year         = {{2007}},
}