Identification of Iridoid Glucoside Transporters in Catharanthus roseus
(2017) In Plant and Cell Physiology 58(9). p.1507-1518- Abstract
Monoterpenoid indole alkaloids (MIAs) are plant defense compounds and high-value pharmaceuticals. Biosynthesis of the universal MIA precursor, secologanin, is organized between internal phloem-associated parenchyma (IPAP) and epidermis cells. Transporters for intercellular transport of proposed mobile pathway intermediates have remained elusive. Screening of an Arabidopsis thaliana transporter library expressed in Xenopus oocytes identified AtNPF2.9 as a putative iridoid glucoside importer. Eight orthologs were identified in Catharanthus roseus, of which three, CrNPF2.4, CrNPF2.5 and CrNPF2.6, were capable of transporting the iridoid glucosides 7-deoxyloganic acid, loganic acid, loganin and secologanin into oocytes. Based on enzyme... (More)
Monoterpenoid indole alkaloids (MIAs) are plant defense compounds and high-value pharmaceuticals. Biosynthesis of the universal MIA precursor, secologanin, is organized between internal phloem-associated parenchyma (IPAP) and epidermis cells. Transporters for intercellular transport of proposed mobile pathway intermediates have remained elusive. Screening of an Arabidopsis thaliana transporter library expressed in Xenopus oocytes identified AtNPF2.9 as a putative iridoid glucoside importer. Eight orthologs were identified in Catharanthus roseus, of which three, CrNPF2.4, CrNPF2.5 and CrNPF2.6, were capable of transporting the iridoid glucosides 7-deoxyloganic acid, loganic acid, loganin and secologanin into oocytes. Based on enzyme expression data and transporter specificity, we propose that several enzymes of the biosynthetic pathway are present in both IPAP and epidermis cells, and that the three transporters are responsible for transporting not only loganic acid, as previously proposed, but multiple intermediates. Identification of the iridoid glucoside-transporting CrNPFs is an important step toward understanding the complex orchestration of the seco-iridioid pathway.
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- author
- Larsen, Lars Bo ; Fuller, Victoria L ; Pollier, Jacob ; Van Moerkercke, Alex LU ; Schweizer, Fabian ; Payne, Richard J. ; Colinas, Maite ; O'Connor, Sarah E ; Goossens, Alain and Halkier, Barbara A
- publishing date
- 2017-09-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Plant and Cell Physiology
- volume
- 58
- issue
- 9
- pages
- 12 pages
- publisher
- Oxford University Press
- external identifiers
-
- pmid:28922750
- scopus:85046050509
- ISSN
- 1471-9053
- DOI
- 10.1093/pcp/pcx097
- language
- English
- LU publication?
- no
- id
- 6d8bde2a-50ca-41af-861f-ee68946ab1c3
- date added to LUP
- 2017-11-06 11:27:34
- date last changed
- 2024-03-31 18:13:29
@article{6d8bde2a-50ca-41af-861f-ee68946ab1c3,
abstract = {{<p>Monoterpenoid indole alkaloids (MIAs) are plant defense compounds and high-value pharmaceuticals. Biosynthesis of the universal MIA precursor, secologanin, is organized between internal phloem-associated parenchyma (IPAP) and epidermis cells. Transporters for intercellular transport of proposed mobile pathway intermediates have remained elusive. Screening of an Arabidopsis thaliana transporter library expressed in Xenopus oocytes identified AtNPF2.9 as a putative iridoid glucoside importer. Eight orthologs were identified in Catharanthus roseus, of which three, CrNPF2.4, CrNPF2.5 and CrNPF2.6, were capable of transporting the iridoid glucosides 7-deoxyloganic acid, loganic acid, loganin and secologanin into oocytes. Based on enzyme expression data and transporter specificity, we propose that several enzymes of the biosynthetic pathway are present in both IPAP and epidermis cells, and that the three transporters are responsible for transporting not only loganic acid, as previously proposed, but multiple intermediates. Identification of the iridoid glucoside-transporting CrNPFs is an important step toward understanding the complex orchestration of the seco-iridioid pathway.</p>}},
author = {{Larsen, Lars Bo and Fuller, Victoria L and Pollier, Jacob and Van Moerkercke, Alex and Schweizer, Fabian and Payne, Richard J. and Colinas, Maite and O'Connor, Sarah E and Goossens, Alain and Halkier, Barbara A}},
issn = {{1471-9053}},
language = {{eng}},
month = {{09}},
number = {{9}},
pages = {{1507--1518}},
publisher = {{Oxford University Press}},
series = {{Plant and Cell Physiology}},
title = {{Identification of Iridoid Glucoside Transporters in Catharanthus roseus}},
url = {{http://dx.doi.org/10.1093/pcp/pcx097}},
doi = {{10.1093/pcp/pcx097}},
volume = {{58}},
year = {{2017}},
}