Increased levels of mitogen activated protein kinase (MAP-K) detected in the injured adult mouse sciatic nerve
(1995) In Neuroscience Letters 200(1). p.33-36- Abstract
Adult mouse sciatic nerves (SNs) with attached dorsal root ganglia (DRG) were analysed for the presence of mitogen activated protein kinase (MAP-K) during normal and regenerative conditions. By immunohistochemistry, MAP-k was found to be present in the normal nerve at low levels in both Schwann cells and DRG nerve cell bodies, with a profoundly increased expression during regeneration. In axonal outgrowth assays, treatment with 2 mM 2-aminopurine (2-AP), a MAP-K antagonist, inhibited the regeneration of axons from the SN as well as from the cultured superior cervical ganglia. The reduced outgrowth was probably not due to toxic effects of the drug since the ganglionic protein synthesis was not inhibited. It is possible that 2-AP... (More)
Adult mouse sciatic nerves (SNs) with attached dorsal root ganglia (DRG) were analysed for the presence of mitogen activated protein kinase (MAP-K) during normal and regenerative conditions. By immunohistochemistry, MAP-k was found to be present in the normal nerve at low levels in both Schwann cells and DRG nerve cell bodies, with a profoundly increased expression during regeneration. In axonal outgrowth assays, treatment with 2 mM 2-aminopurine (2-AP), a MAP-K antagonist, inhibited the regeneration of axons from the SN as well as from the cultured superior cervical ganglia. The reduced outgrowth was probably not due to toxic effects of the drug since the ganglionic protein synthesis was not inhibited. It is possible that 2-AP inteferes with regeneration-related events by inhibition of MAP-K.
(Less)
- author
- Svensson, Bodil ; Ekström, Per LU and Edström, Anders LU
- organization
- publishing date
- 1995-11-10
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- 2-Aminopurine, Adult, Mitogen activated protein kinase, Mouse, Nerve regeneration, Sciatic nerve
- in
- Neuroscience Letters
- volume
- 200
- issue
- 1
- pages
- 4 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0028805095
- pmid:8584260
- ISSN
- 0304-3940
- DOI
- 10.1016/0304-3940(95)12066-D
- language
- English
- LU publication?
- yes
- id
- 759836ee-da97-438a-aab2-34af497f5bd9
- date added to LUP
- 2016-12-07 14:52:47
- date last changed
- 2024-01-04 18:24:47
@article{759836ee-da97-438a-aab2-34af497f5bd9, abstract = {{<p>Adult mouse sciatic nerves (SNs) with attached dorsal root ganglia (DRG) were analysed for the presence of mitogen activated protein kinase (MAP-K) during normal and regenerative conditions. By immunohistochemistry, MAP-k was found to be present in the normal nerve at low levels in both Schwann cells and DRG nerve cell bodies, with a profoundly increased expression during regeneration. In axonal outgrowth assays, treatment with 2 mM 2-aminopurine (2-AP), a MAP-K antagonist, inhibited the regeneration of axons from the SN as well as from the cultured superior cervical ganglia. The reduced outgrowth was probably not due to toxic effects of the drug since the ganglionic protein synthesis was not inhibited. It is possible that 2-AP inteferes with regeneration-related events by inhibition of MAP-K.</p>}}, author = {{Svensson, Bodil and Ekström, Per and Edström, Anders}}, issn = {{0304-3940}}, keywords = {{2-Aminopurine; Adult; Mitogen activated protein kinase; Mouse; Nerve regeneration; Sciatic nerve}}, language = {{eng}}, month = {{11}}, number = {{1}}, pages = {{33--36}}, publisher = {{Elsevier}}, series = {{Neuroscience Letters}}, title = {{Increased levels of mitogen activated protein kinase (MAP-K) detected in the injured adult mouse sciatic nerve}}, url = {{http://dx.doi.org/10.1016/0304-3940(95)12066-D}}, doi = {{10.1016/0304-3940(95)12066-D}}, volume = {{200}}, year = {{1995}}, }