Bacillus subtilis CtaA and CtaB function in haem A biosynthesis
(1993) In Molecular Microbiology 10(1). p.193-201- Abstract
- Haem A, a prosthetic group of many respiratory oxidases, is probably synthesized from haem B (protohaem IX) in a pathway in which haem 0 is an intermediate. Possible roles of the Bacillus subtilis ctaA and ctaB gene products in haem 0 and haem A synthesis were studied. Escherichia coli does not contain haem A. The ctaA gene on plasmids in E. coli resulted in haem A accumulation in membranes. The presence of ctaB together with ctaA increased the amount of haem A found in E. coli. Haem 0 was not detected in wild-type B. subtilis strains. A previously isolated B. subtilis ctaA deletion mutant was found to contain haem B and haem 0, but not haem A. B. subtilis ctaB deletion mutants were constructed and found to lack both haem A and haem 0. The... (More)
- Haem A, a prosthetic group of many respiratory oxidases, is probably synthesized from haem B (protohaem IX) in a pathway in which haem 0 is an intermediate. Possible roles of the Bacillus subtilis ctaA and ctaB gene products in haem 0 and haem A synthesis were studied. Escherichia coli does not contain haem A. The ctaA gene on plasmids in E. coli resulted in haem A accumulation in membranes. The presence of ctaB together with ctaA increased the amount of haem A found in E. coli. Haem 0 was not detected in wild-type B. subtilis strains. A previously isolated B. subtilis ctaA deletion mutant was found to contain haem B and haem 0, but not haem A. B. subtilis ctaB deletion mutants were constructed and found to lack both haem A and haem 0. The results with E. coli and B. subtilis strongly suggest that the B. subtilis CtaA protein functions in haem A synthesis. It is tentatively suggested that it functions in the oxygenation/oxidation of the methyl side group of carbon 8 of haem 0. B. subtilis CtaB, which is homologous to Saccharomyces cerevisiae COX10 and E. coli CyoE, also has a role in haem A synthesis and seems to be required for both cytochrome a and cytochrome o synthesis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/75a28bdb-5356-4974-87ee-e8fae9b738d9
- author
- Svensson, Birgitta ; Lübben, Mattias and Hederstedt, Lars LU
- organization
- publishing date
- 1993
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular Microbiology
- volume
- 10
- issue
- 1
- pages
- 193 - 201
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0027527547
- ISSN
- 1365-2958
- DOI
- 10.1111/j.1365-2958.1993.tb00915.x
- language
- English
- LU publication?
- yes
- id
- 75a28bdb-5356-4974-87ee-e8fae9b738d9
- date added to LUP
- 2017-07-18 10:20:24
- date last changed
- 2021-09-05 04:20:23
@article{75a28bdb-5356-4974-87ee-e8fae9b738d9, abstract = {{Haem A, a prosthetic group of many respiratory oxidases, is probably synthesized from haem B (protohaem IX) in a pathway in which haem 0 is an intermediate. Possible roles of the Bacillus subtilis ctaA and ctaB gene products in haem 0 and haem A synthesis were studied. Escherichia coli does not contain haem A. The ctaA gene on plasmids in E. coli resulted in haem A accumulation in membranes. The presence of ctaB together with ctaA increased the amount of haem A found in E. coli. Haem 0 was not detected in wild-type B. subtilis strains. A previously isolated B. subtilis ctaA deletion mutant was found to contain haem B and haem 0, but not haem A. B. subtilis ctaB deletion mutants were constructed and found to lack both haem A and haem 0. The results with E. coli and B. subtilis strongly suggest that the B. subtilis CtaA protein functions in haem A synthesis. It is tentatively suggested that it functions in the oxygenation/oxidation of the methyl side group of carbon 8 of haem 0. B. subtilis CtaB, which is homologous to Saccharomyces cerevisiae COX10 and E. coli CyoE, also has a role in haem A synthesis and seems to be required for both cytochrome a and cytochrome o synthesis.}}, author = {{Svensson, Birgitta and Lübben, Mattias and Hederstedt, Lars}}, issn = {{1365-2958}}, language = {{eng}}, number = {{1}}, pages = {{193--201}}, publisher = {{Wiley-Blackwell}}, series = {{Molecular Microbiology}}, title = {{<em>Bacillus subtilis</em> CtaA and CtaB function in haem A biosynthesis}}, url = {{http://dx.doi.org/10.1111/j.1365-2958.1993.tb00915.x}}, doi = {{10.1111/j.1365-2958.1993.tb00915.x}}, volume = {{10}}, year = {{1993}}, }