Characterization of mutations in barley fch2 encoding chlorophyllide a oxygenase
(2012) In Plant and Cell Physiology 53(7). p.1232-1246- Abstract
- The barley (Hordeum vulgare L.) mutants fch2 and clo-f2 comprise an allelic group of 14 Chl b-deficient lines. The genetic map position of fch2 corresponds to the physical map position of the gene encoding chlorophyllide a oxygenase. This enzyme converts chlorophyllide a to chlorophyllide b and it is essential for Chl b biosynthesis. The fch2 and clo-f2 barley lines were shown to be mutated in the gene for chlorophyllide a oxygenase. A five-base insertion was found in fch2 and base deletions in clo-f2.101, clo-f2.105, clo-f2.2800 and clo-f2.3613. In clo-f2.105 and clo-f2.108, nonsense base exchanges were discovered. All of these mutations led to a premature stop of translation and none of the mutants formed Chl b. The mutant clo-f2.2807... (More)
- The barley (Hordeum vulgare L.) mutants fch2 and clo-f2 comprise an allelic group of 14 Chl b-deficient lines. The genetic map position of fch2 corresponds to the physical map position of the gene encoding chlorophyllide a oxygenase. This enzyme converts chlorophyllide a to chlorophyllide b and it is essential for Chl b biosynthesis. The fch2 and clo-f2 barley lines were shown to be mutated in the gene for chlorophyllide a oxygenase. A five-base insertion was found in fch2 and base deletions in clo-f2.101, clo-f2.105, clo-f2.2800 and clo-f2.3613. In clo-f2.105 and clo-f2.108, nonsense base exchanges were discovered. All of these mutations led to a premature stop of translation and none of the mutants formed Chl b. The mutant clo-f2.2807 was transcript deficient and formed no Chl b. Missense mutations in clo-f2.102 (leading to the amino acid exchange D495N) and clo-f2.103 (G280D) resulted in a total lack of Chl b, whereas in the missense mutants clo-f2.107 (P419L), clo-f2.109 (A94T), clo-f2.122 (C320Y), clo-f2.123 (A94T), clo-f2.133 (A376V) and clo-f2.181 (L373F) intermediate contents of Chl b were determined. The missense mutations affect conserved residues, and their effect on chlorophyllide a oxygenase is discussed. The mutations in clo-f2.102, clo-f2.103, clo-f2.133 and clo-f2.181 may influence electron transfer as illustrated in the active site of a structural model protein. The changes in clo-f2.107, clo-f2.109, clo-f2.122 and clo-f2.123 may lead to Chlb deficiency by interfering with the regulation of chlorophyllide a oxygenase. The correlation of mutations and phenotypes strongly supports that the barley locus fch2 encodes chlorophyllide a oxygenase. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8001631
- author
- Mueller, A. H. ; Dockter, C. ; Gough, S. P. ; Lundqvist, U. ; von Wettstein, D. and Hansson, Mats LU
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Hordeum/enzymology/*genetics, Genes, Plant, Enzymologic, Gene Expression Regulation, Frameshift Mutation, Electron Transport, Nonsense/genetics/metabolism, Codon, Molecular, Cloning, Plant/genetics/metabolism, Chromosomes, Chromosome Mapping, Chlorophyll/genetics/metabolism, Catalytic Domain, Alleles, Amino Acid Sequence, Molecular Sequence Data, *Mutation, Missense, Oxygenases/*genetics/metabolism, Phenotype, Plant Proteins/genetics/*metabolism, Protein Structure, Tertiary, Sequence Alignment, Synteny
- in
- Plant and Cell Physiology
- volume
- 53
- issue
- 7
- pages
- 1232 - 1246
- publisher
- Oxford University Press
- external identifiers
-
- scopus:84863938407
- pmid:22537757
- ISSN
- 1471-9053
- DOI
- 10.1093/pcp/pcs062
- language
- English
- LU publication?
- no
- additional info
- 7
- id
- f7867a0d-bda9-436c-8d78-a9e2c93ea2cd (old id 8001631)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22537757
- date added to LUP
- 2016-04-01 10:42:34
- date last changed
- 2022-01-26 01:47:08
@article{f7867a0d-bda9-436c-8d78-a9e2c93ea2cd,
abstract = {{The barley (Hordeum vulgare L.) mutants fch2 and clo-f2 comprise an allelic group of 14 Chl b-deficient lines. The genetic map position of fch2 corresponds to the physical map position of the gene encoding chlorophyllide a oxygenase. This enzyme converts chlorophyllide a to chlorophyllide b and it is essential for Chl b biosynthesis. The fch2 and clo-f2 barley lines were shown to be mutated in the gene for chlorophyllide a oxygenase. A five-base insertion was found in fch2 and base deletions in clo-f2.101, clo-f2.105, clo-f2.2800 and clo-f2.3613. In clo-f2.105 and clo-f2.108, nonsense base exchanges were discovered. All of these mutations led to a premature stop of translation and none of the mutants formed Chl b. The mutant clo-f2.2807 was transcript deficient and formed no Chl b. Missense mutations in clo-f2.102 (leading to the amino acid exchange D495N) and clo-f2.103 (G280D) resulted in a total lack of Chl b, whereas in the missense mutants clo-f2.107 (P419L), clo-f2.109 (A94T), clo-f2.122 (C320Y), clo-f2.123 (A94T), clo-f2.133 (A376V) and clo-f2.181 (L373F) intermediate contents of Chl b were determined. The missense mutations affect conserved residues, and their effect on chlorophyllide a oxygenase is discussed. The mutations in clo-f2.102, clo-f2.103, clo-f2.133 and clo-f2.181 may influence electron transfer as illustrated in the active site of a structural model protein. The changes in clo-f2.107, clo-f2.109, clo-f2.122 and clo-f2.123 may lead to Chlb deficiency by interfering with the regulation of chlorophyllide a oxygenase. The correlation of mutations and phenotypes strongly supports that the barley locus fch2 encodes chlorophyllide a oxygenase.}},
author = {{Mueller, A. H. and Dockter, C. and Gough, S. P. and Lundqvist, U. and von Wettstein, D. and Hansson, Mats}},
issn = {{1471-9053}},
keywords = {{Hordeum/enzymology/*genetics; Genes; Plant; Enzymologic; Gene Expression Regulation; Frameshift Mutation; Electron Transport; Nonsense/genetics/metabolism; Codon; Molecular; Cloning; Plant/genetics/metabolism; Chromosomes; Chromosome Mapping; Chlorophyll/genetics/metabolism; Catalytic Domain; Alleles; Amino Acid Sequence; Molecular Sequence Data; *Mutation; Missense; Oxygenases/*genetics/metabolism; Phenotype; Plant Proteins/genetics/*metabolism; Protein Structure; Tertiary; Sequence Alignment; Synteny}},
language = {{eng}},
number = {{7}},
pages = {{1232--1246}},
publisher = {{Oxford University Press}},
series = {{Plant and Cell Physiology}},
title = {{Characterization of mutations in barley fch2 encoding chlorophyllide a oxygenase}},
url = {{http://dx.doi.org/10.1093/pcp/pcs062}},
doi = {{10.1093/pcp/pcs062}},
volume = {{53}},
year = {{2012}},
}