Deletion of the Bacillus subtilis sdh operon
(1987) In FEMS Microbiology Letters 41(2). p.206-206- Abstract
- Plasmid pKIM2 carries the Bacillus subtilis sdh operon and adjacent regions of the bacterial chromosome. The plasmid replicates in Escherichia coli but not in B. subtilis. Different portions of the sdh operon were removed from pKIM2 and replaced by a cat gene derived from pC194. A series of plasmids carrying sdh deletions was thus derived. Plasmid DNA was linearized at restriction sites within the vector part and used to transform B. subtilis to chloramphenicol resistance. The majority of the transformants had a succinate dehydrogenase-negative phenotype and were deleted in the sdh operon as verified by Southern blotting. The B. subtilis deletion mutants were used to determine the functional integrity of cloned sdh genes.
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- author
- Fridén, H ; Hederstedt, Lars LU and Rutberg, Lars
- organization
- publishing date
- 1987
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Succinate dehydrogenase
- in
- FEMS Microbiology Letters
- volume
- 41
- issue
- 2
- pages
- 206 - 206
- publisher
- Oxford University Press
- external identifiers
-
- scopus:0023127404
- ISSN
- 1574-6968
- DOI
- 10.1111/j.1574-6968.1987.tb02197.x
- language
- English
- LU publication?
- yes
- id
- 817a5e5b-e8e1-4c0d-ba83-3d3edfd53178
- date added to LUP
- 2017-07-18 11:01:36
- date last changed
- 2021-01-03 04:58:55
@article{817a5e5b-e8e1-4c0d-ba83-3d3edfd53178, abstract = {{Plasmid pKIM2 carries the Bacillus subtilis sdh operon and adjacent regions of the bacterial chromosome. The plasmid replicates in Escherichia coli but not in B. subtilis. Different portions of the sdh operon were removed from pKIM2 and replaced by a cat gene derived from pC194. A series of plasmids carrying sdh deletions was thus derived. Plasmid DNA was linearized at restriction sites within the vector part and used to transform B. subtilis to chloramphenicol resistance. The majority of the transformants had a succinate dehydrogenase-negative phenotype and were deleted in the sdh operon as verified by Southern blotting. The B. subtilis deletion mutants were used to determine the functional integrity of cloned sdh genes.}}, author = {{Fridén, H and Hederstedt, Lars and Rutberg, Lars}}, issn = {{1574-6968}}, keywords = {{Succinate dehydrogenase}}, language = {{eng}}, number = {{2}}, pages = {{206--206}}, publisher = {{Oxford University Press}}, series = {{FEMS Microbiology Letters}}, title = {{Deletion of the <em>Bacillus subtilis sdh</em> operon}}, url = {{http://dx.doi.org/10.1111/j.1574-6968.1987.tb02197.x}}, doi = {{10.1111/j.1574-6968.1987.tb02197.x}}, volume = {{41}}, year = {{1987}}, }