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A Probing Feeding Strategy for Escherichia coli Cultures

Axelsson, Jan Peter ; Åkesson, Mats LU and Hagander, Per LU (1999) In Biotechnology Techniques 13. p.523-528
Abstract
A strain-independent feeding strategy for fed-batch cultures of <em>Escherichia coli</em> is presented. The key idea is to exploit a characteristic change in the cell metabolism at the onset of acetate formation. By superimposing short pulses in the glucose feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then used to adjust the feed rate to avoid acetate formation. The feasibility of the strategy is demonstrated by both simulation and experiments.
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Escherichia coli, fed-batch culture, glucose, acetate, feedback control, dissolved oxygen.
in
Biotechnology Techniques
volume
13
pages
523 - 528
publisher
Springer
external identifiers
  • scopus:0032883459
ISSN
0951-208X
language
English
LU publication?
yes
id
99cc0e2d-5c9d-4926-b8a4-f1653b98082e (old id 8497617)
date added to LUP
2016-04-04 14:34:31
date last changed
2022-01-30 02:11:54
@article{99cc0e2d-5c9d-4926-b8a4-f1653b98082e,
  abstract     = {{A strain-independent feeding strategy for fed-batch cultures of &lt;em&gt;Escherichia coli&lt;/em&gt; is presented. The key idea is to exploit a characteristic change in the cell metabolism at the onset of acetate formation. By superimposing short pulses in the glucose feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then used to adjust the feed rate to avoid acetate formation. The feasibility of the strategy is demonstrated by both simulation and experiments.}},
  author       = {{Axelsson, Jan Peter and Åkesson, Mats and Hagander, Per}},
  issn         = {{0951-208X}},
  keywords     = {{Escherichia coli; fed-batch culture; glucose; acetate; feedback control; dissolved oxygen.}},
  language     = {{eng}},
  pages        = {{523--528}},
  publisher    = {{Springer}},
  series       = {{Biotechnology Techniques}},
  title        = {{A Probing Feeding Strategy for <em>Escherichia coli</em> Cultures}},
  volume       = {{13}},
  year         = {{1999}},
}