Overproduction of PIB-Type ATPases.
(2016) In Methods in Molecular Biology 1377. p.29-36- Abstract
- Understanding of the functions and mechanisms of fundamental processes in the cell requires structural information. Structural studies of membrane proteins typically necessitate large amounts of purified and preferably homogenous target protein. Here, we describe a rapid overproduction and purification strategy of a bacterial PIB-type ATPase for isolation of milligrams of target protein per liter Escherichia coli cell culture, with a final quality of the sample which is sufficient for generating high-resolution crystals.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8503916
- author
- Liu, Xiangyu ; Sitsel, Oleg ; Wang, Kaituo and Gourdon, Pontus LU
- organization
- publishing date
- 2016
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Methods in Molecular Biology
- volume
- 1377
- pages
- 29 - 36
- publisher
- Springer
- external identifiers
-
- pmid:26695020
- scopus:84951081850
- pmid:26695020
- ISSN
- 1940-6029
- DOI
- 10.1007/978-1-4939-3179-8_5
- language
- English
- LU publication?
- yes
- id
- 05065485-317b-4905-bcdb-f51e1d678e93 (old id 8503916)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/26695020?dopt=Abstract
- date added to LUP
- 2016-04-04 08:41:12
- date last changed
- 2022-01-29 03:48:23
@article{05065485-317b-4905-bcdb-f51e1d678e93,
abstract = {{Understanding of the functions and mechanisms of fundamental processes in the cell requires structural information. Structural studies of membrane proteins typically necessitate large amounts of purified and preferably homogenous target protein. Here, we describe a rapid overproduction and purification strategy of a bacterial PIB-type ATPase for isolation of milligrams of target protein per liter Escherichia coli cell culture, with a final quality of the sample which is sufficient for generating high-resolution crystals.}},
author = {{Liu, Xiangyu and Sitsel, Oleg and Wang, Kaituo and Gourdon, Pontus}},
issn = {{1940-6029}},
language = {{eng}},
pages = {{29--36}},
publisher = {{Springer}},
series = {{Methods in Molecular Biology}},
title = {{Overproduction of PIB-Type ATPases.}},
url = {{http://dx.doi.org/10.1007/978-1-4939-3179-8_5}},
doi = {{10.1007/978-1-4939-3179-8_5}},
volume = {{1377}},
year = {{2016}},
}