In vitro tumorigenic assay : colony forming assay for cancer stem cells
(2018) In Methods in Molecular Biology 1692. p.89-95- Abstract
Colony forming or clonogenic assay is an in vitro quantitative technique to examine the capability of a single cell to grow into a large colony through clonal expansion. Clonogenic activity is a sensitive indicator of undifferentiated cancer stem cells. Here, we described the colony forming ability of the isolated breast cancer stem cells from the total population of cancer cells using double-layered, soft agarose-based assay. This method demonstrates that cancer stem cells can survive and generate colony growth in an anchorage-independent culture model. The 0.005% crystal violet solution is used in this assay to visualize the generated colonies.
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https://lup.lub.lu.se/record/87707f6b-cec7-4f70-af0f-5ab7cd4bd2d3
- author
- Rajendran, Vijayalakshmi and Jain, Mayur Vilas LU
- organization
- publishing date
- 2018
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Agarose, Cancer stem cell, Clonogenic assay, Colony growth, Crystal violet
- host publication
- Methods in Molecular Biology
- series title
- Methods in Molecular Biology
- volume
- 1692
- pages
- 7 pages
- publisher
- Humana Press
- external identifiers
-
- pmid:28986889
- scopus:85030751988
- ISSN
- 10643745
- DOI
- 10.1007/978-1-4939-7401-6_8
- language
- English
- LU publication?
- yes
- id
- 87707f6b-cec7-4f70-af0f-5ab7cd4bd2d3
- date added to LUP
- 2017-10-18 07:19:14
- date last changed
- 2024-04-14 20:25:17
@inbook{87707f6b-cec7-4f70-af0f-5ab7cd4bd2d3,
abstract = {{<p>Colony forming or clonogenic assay is an in vitro quantitative technique to examine the capability of a single cell to grow into a large colony through clonal expansion. Clonogenic activity is a sensitive indicator of undifferentiated cancer stem cells. Here, we described the colony forming ability of the isolated breast cancer stem cells from the total population of cancer cells using double-layered, soft agarose-based assay. This method demonstrates that cancer stem cells can survive and generate colony growth in an anchorage-independent culture model. The 0.005% crystal violet solution is used in this assay to visualize the generated colonies.</p>}},
author = {{Rajendran, Vijayalakshmi and Jain, Mayur Vilas}},
booktitle = {{Methods in Molecular Biology}},
issn = {{10643745}},
keywords = {{Agarose; Cancer stem cell; Clonogenic assay; Colony growth; Crystal violet}},
language = {{eng}},
pages = {{89--95}},
publisher = {{Humana Press}},
series = {{Methods in Molecular Biology}},
title = {{In vitro tumorigenic assay : colony forming assay for cancer stem cells}},
url = {{http://dx.doi.org/10.1007/978-1-4939-7401-6_8}},
doi = {{10.1007/978-1-4939-7401-6_8}},
volume = {{1692}},
year = {{2018}},
}