A new method for isolating physiologically active Mg-protoporphyrin
(2007) In Plant Physiology and Biochemistry 45(12). p.932-936- Abstract
- Mg-protoporphyrin monomethyl ester (MPE) is a biosynthetic intermediate of chlorophyll and converted by MPE cyclase to protochlorophyllide.
Limited availability of MPE has so far hampered cyclase research. In a new, simplified, method MPE was prepared from freeze dried bchE
mutant Rhodobacter capsulatus DB575 cells by extraction with acetone/H2O/25% NH3. Isolated MPE was identified by absorption and fluorescence
spectroscopy, and its purity was analyzed by HPLC. The extracted MPE was dried and redissolved in buffered DMSO and its substrate
activity is shown by enzymatic cyclase assays. A linear time course was observed for MPE conversion to protochlorophyllide by enzymes from
barley etioplasts.... (More) - Mg-protoporphyrin monomethyl ester (MPE) is a biosynthetic intermediate of chlorophyll and converted by MPE cyclase to protochlorophyllide.
Limited availability of MPE has so far hampered cyclase research. In a new, simplified, method MPE was prepared from freeze dried bchE
mutant Rhodobacter capsulatus DB575 cells by extraction with acetone/H2O/25% NH3. Isolated MPE was identified by absorption and fluorescence
spectroscopy, and its purity was analyzed by HPLC. The extracted MPE was dried and redissolved in buffered DMSO and its substrate
activity is shown by enzymatic cyclase assays. A linear time course was observed for MPE conversion to protochlorophyllide by enzymes from
barley etioplasts. Our innovation of freeze drying the R. capsulatus cells before extraction provides a high yield method for MPE, which is significantly
faster and more reproducible than previous extraction methods. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/935809
- author
- Gough, Simon LU ; Rzeznicka, Kamila LU ; Peterson Wulff, Ragna LU ; da Cruz Francisco, José LU ; Hansson, Andreas LU ; Jensen, Poul Erik and Hansson, Mats LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Mg-protoporphyrin monomethyl ester, MPE, Hordeum vulgare, Cyclase, Rhodobacter capsulatus, Chlorophyll biosynthesis
- in
- Plant Physiology and Biochemistry
- volume
- 45
- issue
- 12
- pages
- 932 - 936
- publisher
- Elsevier
- external identifiers
-
- wos:000252077500009
- scopus:36248975616
- ISSN
- 1873-2690
- DOI
- 10.1016/j.plaphy.2007.09.001
- language
- English
- LU publication?
- yes
- id
- e02f7c61-6316-4ed0-9f5d-bd37fa0beea5 (old id 935809)
- date added to LUP
- 2016-04-01 16:58:45
- date last changed
- 2022-01-28 23:28:33
@article{e02f7c61-6316-4ed0-9f5d-bd37fa0beea5,
abstract = {{Mg-protoporphyrin monomethyl ester (MPE) is a biosynthetic intermediate of chlorophyll and converted by MPE cyclase to protochlorophyllide.<br/><br>
Limited availability of MPE has so far hampered cyclase research. In a new, simplified, method MPE was prepared from freeze dried bchE<br/><br>
mutant Rhodobacter capsulatus DB575 cells by extraction with acetone/H2O/25% NH3. Isolated MPE was identified by absorption and fluorescence<br/><br>
spectroscopy, and its purity was analyzed by HPLC. The extracted MPE was dried and redissolved in buffered DMSO and its substrate<br/><br>
activity is shown by enzymatic cyclase assays. A linear time course was observed for MPE conversion to protochlorophyllide by enzymes from<br/><br>
barley etioplasts. Our innovation of freeze drying the R. capsulatus cells before extraction provides a high yield method for MPE, which is significantly<br/><br>
faster and more reproducible than previous extraction methods.}},
author = {{Gough, Simon and Rzeznicka, Kamila and Peterson Wulff, Ragna and da Cruz Francisco, José and Hansson, Andreas and Jensen, Poul Erik and Hansson, Mats}},
issn = {{1873-2690}},
keywords = {{Mg-protoporphyrin monomethyl ester; MPE; Hordeum vulgare; Cyclase; Rhodobacter capsulatus; Chlorophyll biosynthesis}},
language = {{eng}},
number = {{12}},
pages = {{932--936}},
publisher = {{Elsevier}},
series = {{Plant Physiology and Biochemistry}},
title = {{A new method for isolating physiologically active Mg-protoporphyrin}},
url = {{http://dx.doi.org/10.1016/j.plaphy.2007.09.001}},
doi = {{10.1016/j.plaphy.2007.09.001}},
volume = {{45}},
year = {{2007}},
}