Survival and functional recovery of transplanted human dopaminergic neurons into hemiparkinsonian rats depend on the cannula size of the implantation instrument
(2008) In Journal of Neuroscience Methods 169(1). p.128-134- Abstract
Promising therapeutic strategies for neurodegenerative diseases such as Parkinson's disease include replacement of lost striatal dopaminergic neurons by grafting of embryonic mesencephalic cells. However, the poor survival of the transplanted tissue still limits transplantation of these cells into the human brain in a larger number of patients. We addressed the question, if the diameter of the transplantation cannulas has an effect on the number of surviving transplanted human embryonic mesencephalic cells into the striatum of 6-OHDA lesioned rats. We report a significantly higher number of surviving human cells using an ultrathin micropipette compared to cannulas with wider diameters. Importantly, higher numbers of surviving cells also... (More)
Promising therapeutic strategies for neurodegenerative diseases such as Parkinson's disease include replacement of lost striatal dopaminergic neurons by grafting of embryonic mesencephalic cells. However, the poor survival of the transplanted tissue still limits transplantation of these cells into the human brain in a larger number of patients. We addressed the question, if the diameter of the transplantation cannulas has an effect on the number of surviving transplanted human embryonic mesencephalic cells into the striatum of 6-OHDA lesioned rats. We report a significantly higher number of surviving human cells using an ultrathin micropipette compared to cannulas with wider diameters. Importantly, higher numbers of surviving cells also correlated with a behavioral recovery of the hemiparkinsonian rats.
(Less)
- author
- Steiner, Barbara ; Winter, Christine ; Blumensath, Stefan ; Paul, Gesine LU ; Harnack, Daniel ; Nikkhah, Guido and Kupsch, Andreas
- publishing date
- 2008-03-30
- type
- Contribution to journal
- publication status
- published
- keywords
- Cannula size, Human dopaminergic cells, Transplantation
- in
- Journal of Neuroscience Methods
- volume
- 169
- issue
- 1
- pages
- 7 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:18241930
- scopus:39749112525
- ISSN
- 0165-0270
- DOI
- 10.1016/j.jneumeth.2007.11.032
- language
- English
- LU publication?
- no
- id
- 94d4cacf-ba28-41f7-99c4-ac64dd18a0b6
- date added to LUP
- 2017-05-18 12:49:47
- date last changed
- 2024-01-13 21:19:00
@article{94d4cacf-ba28-41f7-99c4-ac64dd18a0b6, abstract = {{<p>Promising therapeutic strategies for neurodegenerative diseases such as Parkinson's disease include replacement of lost striatal dopaminergic neurons by grafting of embryonic mesencephalic cells. However, the poor survival of the transplanted tissue still limits transplantation of these cells into the human brain in a larger number of patients. We addressed the question, if the diameter of the transplantation cannulas has an effect on the number of surviving transplanted human embryonic mesencephalic cells into the striatum of 6-OHDA lesioned rats. We report a significantly higher number of surviving human cells using an ultrathin micropipette compared to cannulas with wider diameters. Importantly, higher numbers of surviving cells also correlated with a behavioral recovery of the hemiparkinsonian rats.</p>}}, author = {{Steiner, Barbara and Winter, Christine and Blumensath, Stefan and Paul, Gesine and Harnack, Daniel and Nikkhah, Guido and Kupsch, Andreas}}, issn = {{0165-0270}}, keywords = {{Cannula size; Human dopaminergic cells; Transplantation}}, language = {{eng}}, month = {{03}}, number = {{1}}, pages = {{128--134}}, publisher = {{Elsevier}}, series = {{Journal of Neuroscience Methods}}, title = {{Survival and functional recovery of transplanted human dopaminergic neurons into hemiparkinsonian rats depend on the cannula size of the implantation instrument}}, url = {{http://dx.doi.org/10.1016/j.jneumeth.2007.11.032}}, doi = {{10.1016/j.jneumeth.2007.11.032}}, volume = {{169}}, year = {{2008}}, }