The lipophilic hapten parthenolide induces interferon-gamma and interleukin-13 production by peripheral blood-derived CD8+ T cells from contact allergic subjects in vitro
(2008) In British Journal of Dermatology 158(1). p.70-77- Abstract
- Background Peripheral blood mononuclear cells (PBMC) from persons with contact allergy to nickel react in vitro predominantly with nickel-induced CD4+ T cell-mediated production of both T-cell type 1 and 2 cytokines. Objectives The aim of the present study was to investigate if the contact allergen parthenolide, a sesquiterpene lactone of lipophilic character, elicits an immune response which differs from that induced by water-soluble nickel ions. Patients and methods Ten allergic subjects with strong (n = 6), moderate (n = 2), or weak (n = 2) patch-test reactivity to parthenolide and five patch test-negative control subjects participated in the study. PBMC from the subjects were analysed for in vitro reactivity with parthenolide by an... (More)
- Background Peripheral blood mononuclear cells (PBMC) from persons with contact allergy to nickel react in vitro predominantly with nickel-induced CD4+ T cell-mediated production of both T-cell type 1 and 2 cytokines. Objectives The aim of the present study was to investigate if the contact allergen parthenolide, a sesquiterpene lactone of lipophilic character, elicits an immune response which differs from that induced by water-soluble nickel ions. Patients and methods Ten allergic subjects with strong (n = 6), moderate (n = 2), or weak (n = 2) patch-test reactivity to parthenolide and five patch test-negative control subjects participated in the study. PBMC from the subjects were analysed for in vitro reactivity with parthenolide by an enzyme-linked immunospot (ELISpot) assay, measuring cytokine production at the single-cell level. Results The allergic group, but not the control group, responded to parthenolide with increased numbers of cells producing interferon (IFN)-gamma, interleukin (IL)-2, IL-4, IL-5 (P < 0.05 for all) and IL-13 (P < 0.01). The responses manifested by T-cell type 1 (IFN-gamma and IL-2) and type 2 (IL-4, IL-5 and IL-13) cytokines were positively correlated between cytokines. Subjects with a strong or moderate, but not weak or negative, patch-test reaction displayed detectable in vitro responses. In contrast to the CD4+ T cell-mediated peripheral reactivity induced by nickel, cell depletion experiments identified the parthenolide-reactive IFN-gamma- and IL-13-producing cells as CD8+ T cells. Conclusions The finding that the PBMC reactivity to parthenolide in humans involves a CD8+ T cell-mediated type 1 and 2 cytokine response warrants further studies on the relationship between the chemical nature of a hapten and the resulting immune response. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/966385
- author
- Wahlkvist, H. ; Masjedi, K. ; Gruvberger, Birgitta LU ; Zuber, B. ; Karlberg, A. -T. ; Bruze, Magnus LU and Ahlborg, N.
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- ELISpot, parthenolide, sesquiterpene lactone, cytokine, contact allergy
- in
- British Journal of Dermatology
- volume
- 158
- issue
- 1
- pages
- 70 - 77
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000251562900010
- scopus:36849008570
- pmid:17986299
- ISSN
- 1365-2133
- DOI
- 10.1111/j.1365-2133.2007.08287.x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Occupational and Environmental Dermatology Unit (013241310), Pediatrics/Urology/Gynecology/Endocrinology (013240400)
- id
- e982f116-eeae-4138-8cd8-d088f6fe050a (old id 966385)
- date added to LUP
- 2016-04-01 11:59:24
- date last changed
- 2022-03-28 18:39:24
@article{e982f116-eeae-4138-8cd8-d088f6fe050a,
abstract = {{Background Peripheral blood mononuclear cells (PBMC) from persons with contact allergy to nickel react in vitro predominantly with nickel-induced CD4+ T cell-mediated production of both T-cell type 1 and 2 cytokines. Objectives The aim of the present study was to investigate if the contact allergen parthenolide, a sesquiterpene lactone of lipophilic character, elicits an immune response which differs from that induced by water-soluble nickel ions. Patients and methods Ten allergic subjects with strong (n = 6), moderate (n = 2), or weak (n = 2) patch-test reactivity to parthenolide and five patch test-negative control subjects participated in the study. PBMC from the subjects were analysed for in vitro reactivity with parthenolide by an enzyme-linked immunospot (ELISpot) assay, measuring cytokine production at the single-cell level. Results The allergic group, but not the control group, responded to parthenolide with increased numbers of cells producing interferon (IFN)-gamma, interleukin (IL)-2, IL-4, IL-5 (P < 0.05 for all) and IL-13 (P < 0.01). The responses manifested by T-cell type 1 (IFN-gamma and IL-2) and type 2 (IL-4, IL-5 and IL-13) cytokines were positively correlated between cytokines. Subjects with a strong or moderate, but not weak or negative, patch-test reaction displayed detectable in vitro responses. In contrast to the CD4+ T cell-mediated peripheral reactivity induced by nickel, cell depletion experiments identified the parthenolide-reactive IFN-gamma- and IL-13-producing cells as CD8+ T cells. Conclusions The finding that the PBMC reactivity to parthenolide in humans involves a CD8+ T cell-mediated type 1 and 2 cytokine response warrants further studies on the relationship between the chemical nature of a hapten and the resulting immune response.}},
author = {{Wahlkvist, H. and Masjedi, K. and Gruvberger, Birgitta and Zuber, B. and Karlberg, A. -T. and Bruze, Magnus and Ahlborg, N.}},
issn = {{1365-2133}},
keywords = {{ELISpot; parthenolide; sesquiterpene lactone; cytokine; contact allergy}},
language = {{eng}},
number = {{1}},
pages = {{70--77}},
publisher = {{Wiley-Blackwell}},
series = {{British Journal of Dermatology}},
title = {{The lipophilic hapten parthenolide induces interferon-gamma and interleukin-13 production by peripheral blood-derived CD8+ T cells from contact allergic subjects in vitro}},
url = {{http://dx.doi.org/10.1111/j.1365-2133.2007.08287.x}},
doi = {{10.1111/j.1365-2133.2007.08287.x}},
volume = {{158}},
year = {{2008}},
}