Swedish moist snuff accelerates gastric cancer development in Helicobacter pylori-infected wild-type and gastrin transgenic mice
(2007) In Carcinogenesis 28(9). p.2041-2046- Abstract
- The Swedish variant of moist oral smokeless tobacco (snus) is popular in Sweden and Norway, banned from sale within the European Union and is currently being introduced in USA. The aim of the present study was to determine if snus is carcinogenic to the stomach, particularly in Helicobacter pylori (H.P.)-infected hosts at increased risk for gastric cancer development. Snus (General (TM); Swedish Match, Sweden) was mixed with powdered standard mouse chow at a concentration of 5-9% (wt/wt) and given to wild-type (WT, FVB) and gastrin transgenic (INS-GAS, FVB) mice for 6 months with or without H.P. (strain 67:21, CagA(+), VacA(+)) infection. At necropsy, pathological evaluation of stomachs from uninfected snus-treated WT mice showed mild... (More)
- The Swedish variant of moist oral smokeless tobacco (snus) is popular in Sweden and Norway, banned from sale within the European Union and is currently being introduced in USA. The aim of the present study was to determine if snus is carcinogenic to the stomach, particularly in Helicobacter pylori (H.P.)-infected hosts at increased risk for gastric cancer development. Snus (General (TM); Swedish Match, Sweden) was mixed with powdered standard mouse chow at a concentration of 5-9% (wt/wt) and given to wild-type (WT, FVB) and gastrin transgenic (INS-GAS, FVB) mice for 6 months with or without H.P. (strain 67:21, CagA(+), VacA(+)) infection. At necropsy, pathological evaluation of stomachs from uninfected snus-treated WT mice showed mild morphological changes, whereas 50% snus-treated INS-GAS mice developed carcinoma in situ (CIS), compared with 25% not exposed to snus. When snus was given to H.P.-infected mice, 9 of 17 WT mice developed CIS with intramucosal invasion, and the remaining 8 of 17 WT mice developed high-grade dysplasia (score > 1.5) that was associated with increased gastritis, epithelial defects, oxyntic atrophy, hyperplasia and intestinal metaplasia. Twelve of 12 H.P.-infected INS-GAS mice developed CIS with intramucosal invasion and submucosal herniation. We suggest that snus is a potential gastric carcinogen in mice. The development of CIS was associated with increased rates of the epithelial cell proliferation and apoptosis, common features of gastric carcinogenesis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/974399
- author
- Stenstrom, Bjorn ; Zhao, Chun-Mei ; Rogers, Arlin B. ; Nilsson, Hans-Olof LU ; Sturegård, Erik LU ; Lundgren, Steinar ; Fox, James G. ; Wang, Timothy C. ; Wadström, Torkel LU and Chen, Duan
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Carcinogenesis
- volume
- 28
- issue
- 9
- pages
- 2041 - 2046
- publisher
- Oxford University Press
- external identifiers
-
- wos:000250676300028
- scopus:34848831193
- pmid:17389611
- ISSN
- 0143-3334
- DOI
- 10.1093/carcin/bgm071
- language
- English
- LU publication?
- yes
- id
- 76956506-9cc6-4f05-afe1-705326d067b8 (old id 974399)
- date added to LUP
- 2016-04-01 12:31:34
- date last changed
- 2022-03-29 02:02:50
@article{76956506-9cc6-4f05-afe1-705326d067b8,
abstract = {{The Swedish variant of moist oral smokeless tobacco (snus) is popular in Sweden and Norway, banned from sale within the European Union and is currently being introduced in USA. The aim of the present study was to determine if snus is carcinogenic to the stomach, particularly in Helicobacter pylori (H.P.)-infected hosts at increased risk for gastric cancer development. Snus (General (TM); Swedish Match, Sweden) was mixed with powdered standard mouse chow at a concentration of 5-9% (wt/wt) and given to wild-type (WT, FVB) and gastrin transgenic (INS-GAS, FVB) mice for 6 months with or without H.P. (strain 67:21, CagA(+), VacA(+)) infection. At necropsy, pathological evaluation of stomachs from uninfected snus-treated WT mice showed mild morphological changes, whereas 50% snus-treated INS-GAS mice developed carcinoma in situ (CIS), compared with 25% not exposed to snus. When snus was given to H.P.-infected mice, 9 of 17 WT mice developed CIS with intramucosal invasion, and the remaining 8 of 17 WT mice developed high-grade dysplasia (score > 1.5) that was associated with increased gastritis, epithelial defects, oxyntic atrophy, hyperplasia and intestinal metaplasia. Twelve of 12 H.P.-infected INS-GAS mice developed CIS with intramucosal invasion and submucosal herniation. We suggest that snus is a potential gastric carcinogen in mice. The development of CIS was associated with increased rates of the epithelial cell proliferation and apoptosis, common features of gastric carcinogenesis.}},
author = {{Stenstrom, Bjorn and Zhao, Chun-Mei and Rogers, Arlin B. and Nilsson, Hans-Olof and Sturegård, Erik and Lundgren, Steinar and Fox, James G. and Wang, Timothy C. and Wadström, Torkel and Chen, Duan}},
issn = {{0143-3334}},
language = {{eng}},
number = {{9}},
pages = {{2041--2046}},
publisher = {{Oxford University Press}},
series = {{Carcinogenesis}},
title = {{Swedish moist snuff accelerates gastric cancer development in Helicobacter pylori-infected wild-type and gastrin transgenic mice}},
url = {{http://dx.doi.org/10.1093/carcin/bgm071}},
doi = {{10.1093/carcin/bgm071}},
volume = {{28}},
year = {{2007}},
}