Heterokaryon-based reprogramming for pluripotency
Pereira, Carlos Filipe LU
and Fisher, Amanda G.
(2009)
SUPPL 9.
p.1-14
- Abstract
Embryonic stem (ES) cells have the ability to self-renew, execute multiple lineage paths, and dominantly reprogram differentiated cells upon cell fusion. Here, we describe an approach that reprograms human B lymphocytes toward pluripotency by generating inter-species heterokaryons with mouse ES cells. This induces a human ES-specific gene expression profile, in which the extent and the rapidity of conversion allows us to compare the capacity of different mouse ES cell lines to dominantly induce pluripotency. This approach, coupled with pharmacological inhibition, gene knock-out, or knockdown permits factors that are required to directly induce reprogramming to be defined individually, as well as in combination. Experimental... (More)
Embryonic stem (ES) cells have the ability to self-renew, execute multiple lineage paths, and dominantly reprogram differentiated cells upon cell fusion. Here, we describe an approach that reprograms human B lymphocytes toward pluripotency by generating inter-species heterokaryons with mouse ES cells. This induces a human ES-specific gene expression profile, in which the extent and the rapidity of conversion allows us to compare the capacity of different mouse ES cell lines to dominantly induce pluripotency. This approach, coupled with pharmacological inhibition, gene knock-out, or knockdown permits factors that are required to directly induce reprogramming to be defined individually, as well as in combination. Experimental heterokaryons provide a simple and tractable approach to address the mechanisms underlying direct reprogramming to pluripotency. The procedure requires 5 days to complete.
(Less)
- author
- Pereira, Carlos Filipe
LU
and Fisher, Amanda G.
- publishing date
- 2009
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- keywords
- Cell fusion, Embryonic stem (ES) cell, Heterokaryon, Pluripotency, Reprogramming
- host publication
- Current Protocols in Stem Cell Biology
- volume
- SUPPL 9
- pages
- 4 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:65149087887
- pmid:19382123
- ISBN
- 9780470151808
- DOI
- 10.1002/9780470151808.sc04b01s9
- language
- English
- LU publication?
- no
- id
- ab65c501-15dc-4a21-8115-ba6bba881a8b
- date added to LUP
- 2017-10-02 17:31:13
- date last changed
- 2024-04-14 19:37:45
@inbook{ab65c501-15dc-4a21-8115-ba6bba881a8b,
abstract = {{<p>Embryonic stem (ES) cells have the ability to self-renew, execute multiple lineage paths, and dominantly reprogram differentiated cells upon cell fusion. Here, we describe an approach that reprograms human B lymphocytes toward pluripotency by generating inter-species heterokaryons with mouse ES cells. This induces a human ES-specific gene expression profile, in which the extent and the rapidity of conversion allows us to compare the capacity of different mouse ES cell lines to dominantly induce pluripotency. This approach, coupled with pharmacological inhibition, gene knock-out, or knockdown permits factors that are required to directly induce reprogramming to be defined individually, as well as in combination. Experimental heterokaryons provide a simple and tractable approach to address the mechanisms underlying direct reprogramming to pluripotency. The procedure requires 5 days to complete.</p>}},
author = {{Pereira, Carlos Filipe and Fisher, Amanda G.}},
booktitle = {{Current Protocols in Stem Cell Biology}},
isbn = {{9780470151808}},
keywords = {{Cell fusion; Embryonic stem (ES) cell; Heterokaryon; Pluripotency; Reprogramming}},
language = {{eng}},
pages = {{1--14}},
publisher = {{John Wiley & Sons Inc.}},
title = {{Heterokaryon-based reprogramming for pluripotency}},
url = {{http://dx.doi.org/10.1002/9780470151808.sc04b01s9}},
doi = {{10.1002/9780470151808.sc04b01s9}},
volume = {{SUPPL 9}},
year = {{2009}},
}