MicroRNA expression profiling of the porcine developing brain
Podolska, Agnieszka ; Kaczkowski, Bogumil ; Kamp Busk, Peter ; Søkilde, Rolf LU
; Litman, Thomas
; Fredholm, Merete
and Cirera, Susanna
(2011)
In PLoS ONE
6(1).
- Abstract
BACKGROUND: MicroRNAs are small, non-coding RNA molecules that regulate gene expression at the post-transcriptional level and play an important role in the control of developmental and physiological processes. In particular, the developing brain contains an impressive diversity of microRNAs. Most microRNA expression profiling studies have been performed in human or rodents and relatively limited knowledge exists in other mammalian species. The domestic pig is considered to be an excellent, alternate, large mammal model for human-related neurological studies, due to its similarity in both brain development and the growth curve when compared to humans. Considering these similarities, studies examining microRNA expression during porcine... (More)
BACKGROUND: MicroRNAs are small, non-coding RNA molecules that regulate gene expression at the post-transcriptional level and play an important role in the control of developmental and physiological processes. In particular, the developing brain contains an impressive diversity of microRNAs. Most microRNA expression profiling studies have been performed in human or rodents and relatively limited knowledge exists in other mammalian species. The domestic pig is considered to be an excellent, alternate, large mammal model for human-related neurological studies, due to its similarity in both brain development and the growth curve when compared to humans. Considering these similarities, studies examining microRNA expression during porcine brain development could potentially be used to predict the expression profile and role of microRNAs in the human brain.
METHODOLOGY/PRINCIPAL FINDINGS: MicroRNA expression profiling by use of microRNA microarrays and qPCR was performed on the porcine developing brain. Our results show that microRNA expression is regulated in a developmentally stage-specific, as well as a tissue-specific manner. Numerous developmental stage or tissue-specific microRNAs including, miR-17, miR-18a, miR-29c, miR-106a, miR-135a and b, miR-221 and miR-222 were found by microarray analysis. Expression profiles of selected candidates were confirmed by qPCR.
CONCLUSIONS/SIGNIFICANCE: The differential expression of specific microRNAs in fetal versus postnatal samples suggests that they likely play an important role in the regulation of developmental and physiological processes during brain development. The data presented here supports the notion that microRNAs act as post-transcriptional switches which may regulate gene expression when required.
(Less)
- author
- Podolska, Agnieszka
; Kaczkowski, Bogumil
; Kamp Busk, Peter
; Søkilde, Rolf
LU
; Litman, Thomas
; Fredholm, Merete
and Cirera, Susanna
- publishing date
- 2011-01-06
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Brain, Gene Expression Profiling, Gene Expression Regulation, Developmental, MicroRNAs, Sus scrofa, Journal Article, Research Support, Non-U.S. Gov't
- in
- PLoS ONE
- volume
- 6
- issue
- 1
- article number
- e14494
- publisher
- Public Library of Science (PLoS)
- external identifiers
-
- pmid:21253018
- scopus:79251577674
- ISSN
- 1932-6203
- DOI
- 10.1371/journal.pone.0014494
- language
- English
- LU publication?
- no
- id
- af267c63-f039-4ced-b6b0-efd62aaae62c
- date added to LUP
- 2017-09-01 14:30:55
- date last changed
- 2024-03-31 16:03:15
@article{af267c63-f039-4ced-b6b0-efd62aaae62c,
abstract = {{<p>BACKGROUND: MicroRNAs are small, non-coding RNA molecules that regulate gene expression at the post-transcriptional level and play an important role in the control of developmental and physiological processes. In particular, the developing brain contains an impressive diversity of microRNAs. Most microRNA expression profiling studies have been performed in human or rodents and relatively limited knowledge exists in other mammalian species. The domestic pig is considered to be an excellent, alternate, large mammal model for human-related neurological studies, due to its similarity in both brain development and the growth curve when compared to humans. Considering these similarities, studies examining microRNA expression during porcine brain development could potentially be used to predict the expression profile and role of microRNAs in the human brain.</p><p>METHODOLOGY/PRINCIPAL FINDINGS: MicroRNA expression profiling by use of microRNA microarrays and qPCR was performed on the porcine developing brain. Our results show that microRNA expression is regulated in a developmentally stage-specific, as well as a tissue-specific manner. Numerous developmental stage or tissue-specific microRNAs including, miR-17, miR-18a, miR-29c, miR-106a, miR-135a and b, miR-221 and miR-222 were found by microarray analysis. Expression profiles of selected candidates were confirmed by qPCR.</p><p>CONCLUSIONS/SIGNIFICANCE: The differential expression of specific microRNAs in fetal versus postnatal samples suggests that they likely play an important role in the regulation of developmental and physiological processes during brain development. The data presented here supports the notion that microRNAs act as post-transcriptional switches which may regulate gene expression when required.</p>}},
author = {{Podolska, Agnieszka and Kaczkowski, Bogumil and Kamp Busk, Peter and Søkilde, Rolf and Litman, Thomas and Fredholm, Merete and Cirera, Susanna}},
issn = {{1932-6203}},
keywords = {{Animals; Brain; Gene Expression Profiling; Gene Expression Regulation, Developmental; MicroRNAs; Sus scrofa; Journal Article; Research Support, Non-U.S. Gov't}},
language = {{eng}},
month = {{01}},
number = {{1}},
publisher = {{Public Library of Science (PLoS)}},
series = {{PLoS ONE}},
title = {{MicroRNA expression profiling of the porcine developing brain}},
url = {{http://dx.doi.org/10.1371/journal.pone.0014494}},
doi = {{10.1371/journal.pone.0014494}},
volume = {{6}},
year = {{2011}},
}