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Adsorption of cellulases on steam-pretreated willow

Galbe, Mats LU ; Eklund, R and Zacchi, Guido LU (1990) In Applied Biochemistry and Biotechnology 24-5. p.87-101
Abstract
The adsorption of cellulases on steam-pretreated willow has been measured for2, 4, and 8 wt% willow and with varying enzyme concentrations (2–100 wt% based on substrate). The enzyme concentration was measured as soluble protein, filter paper activity (FPA), CMC-ase activity, and activity toward willow. The adsorption data were modeled with a Langmuir isotherm. The maximum adsorption capability depends on the method for measurement of the enzyme concentration. The lowest value, 470 mg enzyme/g willow, was obtained for the soluble protein and the highest value, 650 mg/g, for the FPA. For technical applications, a single isotherm can be used for calculation of the adsorption capability of steam-treated willow.
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Biochemistry and Biotechnology
volume
24-5
pages
87 - 101
publisher
Humana Press
external identifiers
  • wos:A1990DE57300010
  • scopus:0025148108
ISSN
1559-0291
DOI
10.1007/BF02920236
language
English
LU publication?
yes
id
b82c2434-ea0d-4bd3-9ccf-e68aa4cc8b77 (old id 3911606)
date added to LUP
2016-04-01 11:43:04
date last changed
2023-09-01 04:32:54
@article{b82c2434-ea0d-4bd3-9ccf-e68aa4cc8b77,
  abstract     = {{The adsorption of cellulases on steam-pretreated willow has been measured for2, 4, and 8 wt% willow and with varying enzyme concentrations (2–100 wt% based on substrate). The enzyme concentration was measured as soluble protein, filter paper activity (FPA), CMC-ase activity, and activity toward willow. The adsorption data were modeled with a Langmuir isotherm. The maximum adsorption capability depends on the method for measurement of the enzyme concentration. The lowest value, 470 mg enzyme/g willow, was obtained for the soluble protein and the highest value, 650 mg/g, for the FPA. For technical applications, a single isotherm can be used for calculation of the adsorption capability of steam-treated willow.}},
  author       = {{Galbe, Mats and Eklund, R and Zacchi, Guido}},
  issn         = {{1559-0291}},
  language     = {{eng}},
  pages        = {{87--101}},
  publisher    = {{Humana Press}},
  series       = {{Applied Biochemistry and Biotechnology}},
  title        = {{Adsorption of cellulases on steam-pretreated willow}},
  url          = {{http://dx.doi.org/10.1007/BF02920236}},
  doi          = {{10.1007/BF02920236}},
  volume       = {{24-5}},
  year         = {{1990}},
}