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Substituent distribution and clouding behavior of hydroxypropyl methyl cellulose analyzed using enzymatic degradation

Schagerlöf, Herje LU ; Johansson, Marika ; Richardson, Sara ; Brinkmalm, Gunnar ; Wittgren, Bengt and Tjerneld, Folke LU (2006) In Biomacromolecules 7(12). p.3474-3481
Abstract
The distribution of substituents along the polymer backbone will have a strong influence on the properties of modified cellulose. Endoglucanases were used to degrade three different batches of hydroxypropyl methyl cellulose ( HPMC) derivatives with similar chemical properties. The phase separation of the HPMCs as a function of temperature, i.e., the clouding behavior, was analyzed prior to degradation. The total amount of unsubstituted glucose was determined using total acid hydrolysis followed by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The products after enzymatic degradation were analyzed with size-exclusion chromatography with online multiangle light scattering and refractive index... (More)
The distribution of substituents along the polymer backbone will have a strong influence on the properties of modified cellulose. Endoglucanases were used to degrade three different batches of hydroxypropyl methyl cellulose ( HPMC) derivatives with similar chemical properties. The phase separation of the HPMCs as a function of temperature, i.e., the clouding behavior, was analyzed prior to degradation. The total amount of unsubstituted glucose was determined using total acid hydrolysis followed by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The products after enzymatic degradation were analyzed with size-exclusion chromatography with online multiangle light scattering and refractive index detection and also with reducing end determination. To further characterize the formed products, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was employed for analysis of short-chained oligosaccharides. The different endoglucanases showed varying degradation capability of HPMC derivatives, depending on structure of the active site. The investigated HPMCs had different susceptibility to degradation by the endoglucanases. The results showed a difference in substituent distribution between HPMC batches, which could explain the differing clouding behaviors. The batch with the lowest cloud point was shown to contain a higher number of non-degradable, highly substituted regions. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomacromolecules
volume
7
issue
12
pages
3474 - 3481
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000242701600025
  • scopus:33846321248
ISSN
1526-4602
DOI
10.1021/bm0604799
language
English
LU publication?
yes
id
ba49c0c3-619b-4d23-a8d1-d50fc1fcfa78 (old id 683504)
date added to LUP
2016-04-01 12:07:40
date last changed
2022-01-26 23:14:01
@article{ba49c0c3-619b-4d23-a8d1-d50fc1fcfa78,
  abstract     = {{The distribution of substituents along the polymer backbone will have a strong influence on the properties of modified cellulose. Endoglucanases were used to degrade three different batches of hydroxypropyl methyl cellulose ( HPMC) derivatives with similar chemical properties. The phase separation of the HPMCs as a function of temperature, i.e., the clouding behavior, was analyzed prior to degradation. The total amount of unsubstituted glucose was determined using total acid hydrolysis followed by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The products after enzymatic degradation were analyzed with size-exclusion chromatography with online multiangle light scattering and refractive index detection and also with reducing end determination. To further characterize the formed products, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was employed for analysis of short-chained oligosaccharides. The different endoglucanases showed varying degradation capability of HPMC derivatives, depending on structure of the active site. The investigated HPMCs had different susceptibility to degradation by the endoglucanases. The results showed a difference in substituent distribution between HPMC batches, which could explain the differing clouding behaviors. The batch with the lowest cloud point was shown to contain a higher number of non-degradable, highly substituted regions.}},
  author       = {{Schagerlöf, Herje and Johansson, Marika and Richardson, Sara and Brinkmalm, Gunnar and Wittgren, Bengt and Tjerneld, Folke}},
  issn         = {{1526-4602}},
  language     = {{eng}},
  number       = {{12}},
  pages        = {{3474--3481}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Biomacromolecules}},
  title        = {{Substituent distribution and clouding behavior of hydroxypropyl methyl cellulose analyzed using enzymatic degradation}},
  url          = {{http://dx.doi.org/10.1021/bm0604799}},
  doi          = {{10.1021/bm0604799}},
  volume       = {{7}},
  year         = {{2006}},
}