Identification of the ligand of Pru p 3, a peach LTP
(2017) In Plant Molecular Biology 94(1-2). p.33-44- Abstract
Key message: Pru p 3, a peach LTP, is located in pollinated flower styles and secreting downy hairs, transporting a derivative of camptothecin bound to phytosphingosine. Pru p 3 may inhibit a second pollination and may keep away herbivores until seed maturation. Abstract: The allergen Pru p 3, a peach lipid transfer protein, has been well studied. However, its physiological function remains to be elucidated. Our results showed that Pru p 3 usually carries a lipid ligand that play an essential role in its function in plants. Using ESI-qToF, we observed that the ligand was a derivative of camptothecin binding to phytosphingosine, wich that is inserted into the hydrophobic tunnel of the protein. In addition, the described ligand displayed... (More)
Key message: Pru p 3, a peach LTP, is located in pollinated flower styles and secreting downy hairs, transporting a derivative of camptothecin bound to phytosphingosine. Pru p 3 may inhibit a second pollination and may keep away herbivores until seed maturation. Abstract: The allergen Pru p 3, a peach lipid transfer protein, has been well studied. However, its physiological function remains to be elucidated. Our results showed that Pru p 3 usually carries a lipid ligand that play an essential role in its function in plants. Using ESI-qToF, we observed that the ligand was a derivative of camptothecin binding to phytosphingosine, wich that is inserted into the hydrophobic tunnel of the protein. In addition, the described ligand displayed topoisomerase I activity inhibition and self-fluorescence, both recognized as camptothecin properties. During flower development, the highest expression of Pru p 3 was detected in the styles of pollinated flowers, in contrast to its non-expression in unpollinated pistils, where expression decreased after anthesis. During ripening, the expression of Pru p 3 were observed mainly in peel but not in pulp. In this sense, Pru p 3 protein was also localized in trichomes covering the fruit epidermis.
(Less)
- author
- Cubells-Baeza, Nuria ; Gómez-Casado, Cristina LU ; Tordesillas, Leticia ; Ramírez-Castillejo, Carmen ; Garrido-Arandia, María ; González-Melendi, Pablo ; Herrero, María ; Pacios, Luis F. and Díaz-Perales, Araceli
- organization
- publishing date
- 2017-05
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Camptothecin, Flower development, Fruit development, Lipid transfer protein, Pollination, Pru p 3, Secondary metabolites
- in
- Plant Molecular Biology
- volume
- 94
- issue
- 1-2
- pages
- 12 pages
- publisher
- Springer
- external identifiers
-
- scopus:85015257435
- pmid:28299506
- wos:000401893100003
- ISSN
- 0167-4412
- DOI
- 10.1007/s11103-017-0590-z
- language
- English
- LU publication?
- yes
- id
- be2d508d-75ca-4fb3-afac-69783e99ad25
- date added to LUP
- 2017-03-29 12:15:13
- date last changed
- 2024-03-31 06:44:12
@article{be2d508d-75ca-4fb3-afac-69783e99ad25,
abstract = {{<p>Key message: Pru p 3, a peach LTP, is located in pollinated flower styles and secreting downy hairs, transporting a derivative of camptothecin bound to phytosphingosine. Pru p 3 may inhibit a second pollination and may keep away herbivores until seed maturation. Abstract: The allergen Pru p 3, a peach lipid transfer protein, has been well studied. However, its physiological function remains to be elucidated. Our results showed that Pru p 3 usually carries a lipid ligand that play an essential role in its function in plants. Using ESI-qToF, we observed that the ligand was a derivative of camptothecin binding to phytosphingosine, wich that is inserted into the hydrophobic tunnel of the protein. In addition, the described ligand displayed topoisomerase I activity inhibition and self-fluorescence, both recognized as camptothecin properties. During flower development, the highest expression of Pru p 3 was detected in the styles of pollinated flowers, in contrast to its non-expression in unpollinated pistils, where expression decreased after anthesis. During ripening, the expression of Pru p 3 were observed mainly in peel but not in pulp. In this sense, Pru p 3 protein was also localized in trichomes covering the fruit epidermis.</p>}},
author = {{Cubells-Baeza, Nuria and Gómez-Casado, Cristina and Tordesillas, Leticia and Ramírez-Castillejo, Carmen and Garrido-Arandia, María and González-Melendi, Pablo and Herrero, María and Pacios, Luis F. and Díaz-Perales, Araceli}},
issn = {{0167-4412}},
keywords = {{Camptothecin; Flower development; Fruit development; Lipid transfer protein; Pollination; Pru p 3; Secondary metabolites}},
language = {{eng}},
number = {{1-2}},
pages = {{33--44}},
publisher = {{Springer}},
series = {{Plant Molecular Biology}},
title = {{Identification of the ligand of Pru p 3, a peach LTP}},
url = {{http://dx.doi.org/10.1007/s11103-017-0590-z}},
doi = {{10.1007/s11103-017-0590-z}},
volume = {{94}},
year = {{2017}},
}