Altered expression of myelin-associated inhibitors and their receptors after traumatic brain injury in the mouse
Israelsson, Charlotte ; Flygt, Johanna ; Åstrand, Elaine ; Kiwanuka, Olivia ; Bengtsson, Henrik and Marklund, Niklas LU
(2014)
In Restorative Neurology and Neuroscience
32(5).
p.31-717
- Abstract
PURPOSE: When central nervous system axons are injured, regeneration is partly inhibited by myelin-associated inhibitors (MAIs). Following traumatic brain injury (TBI) in the rat, pharmacological neutralisation of the MAIs Nogo-A and myelin-associated glycoprotein (MAG) resulted in improved functional outcome. In contrast, genetic or pharmacological neutralization of the MAI receptors Nogo-66 receptor 1 (NgR1) or paired-immunoglobulin like receptor-B (PirB) showed an unaltered or impaired outcome following TBI in mice. The aim of the present study was thus to evaluate the MAI expression levels following TBI in mice.
METHODS: Quantitative reverse transcriptase PCR (qRT-PCR) was used to measure total RNA isolated from brains of... (More)
PURPOSE: When central nervous system axons are injured, regeneration is partly inhibited by myelin-associated inhibitors (MAIs). Following traumatic brain injury (TBI) in the rat, pharmacological neutralisation of the MAIs Nogo-A and myelin-associated glycoprotein (MAG) resulted in improved functional outcome. In contrast, genetic or pharmacological neutralization of the MAI receptors Nogo-66 receptor 1 (NgR1) or paired-immunoglobulin like receptor-B (PirB) showed an unaltered or impaired outcome following TBI in mice. The aim of the present study was thus to evaluate the MAI expression levels following TBI in mice.
METHODS: Quantitative reverse transcriptase PCR (qRT-PCR) was used to measure total RNA isolated from brains of young adult male C57BL/6 mice at one, three or seven days following controlled cortical impact TBI or sham injury. Hippocampal and neocortical tissue ipsi- and contralateral to the injury was analyzed for Nogo-A, oligodendrocyte-myelin glycoprotein (OMgp), MAG, and the MAI receptors PirB and NgR1, including its co-receptor Lingo1.
RESULTS: Compared to sham-injured controls, PirB neocortical expression was significantly upregulated at one day and NgR1 expression downregulated at seven days post-TBI. In the hippocampus, transcriptional upregulation was observed in Nogo-A (one day post-injury), MAG and PirB at seven days post-injury. In contrast, the hippocampal transcripts of NgR1 and Lingo1 were decreased at seven days post-injury. The expression of OMgp was unaltered at all time points post-injury.
CONCLUSION: These results suggest that early dynamic changes in MAI gene expression occur following TBI in the mouse, particularly in the hippocampus, which may play an inhibitory role for post-injury regeneration and plasticity.
(Less)
- author
- Israelsson, Charlotte
; Flygt, Johanna
; Åstrand, Elaine
; Kiwanuka, Olivia
; Bengtsson, Henrik
and Marklund, Niklas
LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Brain, Brain Injuries, Disease Models, Animal, GPI-Linked Proteins, Gene Expression Regulation, Male, Membrane Proteins, Mice, Mice, Inbred C57BL, Myelin Proteins, Nerve Tissue Proteins, Oligodendrocyte-Myelin Glycoprotein, RNA, Messenger, Receptors, Cell Surface, Receptors, Immunologic, Time Factors, Journal Article, Research Support, Non-U.S. Gov't
- in
- Restorative Neurology and Neuroscience
- volume
- 32
- issue
- 5
- pages
- 31 - 717
- publisher
- IOS Press
- external identifiers
-
- pmid:25079982
- scopus:84907816087
- ISSN
- 1878-3627
- DOI
- 10.3233/RNN-140419
- language
- English
- LU publication?
- no
- id
- c06eabde-cfc6-4e6c-9c31-51ac30d7df3d
- date added to LUP
- 2016-12-09 16:04:57
- date last changed
- 2024-01-04 18:40:02
@article{c06eabde-cfc6-4e6c-9c31-51ac30d7df3d,
abstract = {{<p>PURPOSE: When central nervous system axons are injured, regeneration is partly inhibited by myelin-associated inhibitors (MAIs). Following traumatic brain injury (TBI) in the rat, pharmacological neutralisation of the MAIs Nogo-A and myelin-associated glycoprotein (MAG) resulted in improved functional outcome. In contrast, genetic or pharmacological neutralization of the MAI receptors Nogo-66 receptor 1 (NgR1) or paired-immunoglobulin like receptor-B (PirB) showed an unaltered or impaired outcome following TBI in mice. The aim of the present study was thus to evaluate the MAI expression levels following TBI in mice.</p><p>METHODS: Quantitative reverse transcriptase PCR (qRT-PCR) was used to measure total RNA isolated from brains of young adult male C57BL/6 mice at one, three or seven days following controlled cortical impact TBI or sham injury. Hippocampal and neocortical tissue ipsi- and contralateral to the injury was analyzed for Nogo-A, oligodendrocyte-myelin glycoprotein (OMgp), MAG, and the MAI receptors PirB and NgR1, including its co-receptor Lingo1.</p><p>RESULTS: Compared to sham-injured controls, PirB neocortical expression was significantly upregulated at one day and NgR1 expression downregulated at seven days post-TBI. In the hippocampus, transcriptional upregulation was observed in Nogo-A (one day post-injury), MAG and PirB at seven days post-injury. In contrast, the hippocampal transcripts of NgR1 and Lingo1 were decreased at seven days post-injury. The expression of OMgp was unaltered at all time points post-injury.</p><p>CONCLUSION: These results suggest that early dynamic changes in MAI gene expression occur following TBI in the mouse, particularly in the hippocampus, which may play an inhibitory role for post-injury regeneration and plasticity.</p>}},
author = {{Israelsson, Charlotte and Flygt, Johanna and Åstrand, Elaine and Kiwanuka, Olivia and Bengtsson, Henrik and Marklund, Niklas}},
issn = {{1878-3627}},
keywords = {{Animals; Brain; Brain Injuries; Disease Models, Animal; GPI-Linked Proteins; Gene Expression Regulation; Male; Membrane Proteins; Mice; Mice, Inbred C57BL; Myelin Proteins; Nerve Tissue Proteins; Oligodendrocyte-Myelin Glycoprotein; RNA, Messenger; Receptors, Cell Surface; Receptors, Immunologic; Time Factors; Journal Article; Research Support, Non-U.S. Gov't}},
language = {{eng}},
number = {{5}},
pages = {{31--717}},
publisher = {{IOS Press}},
series = {{Restorative Neurology and Neuroscience}},
title = {{Altered expression of myelin-associated inhibitors and their receptors after traumatic brain injury in the mouse}},
url = {{http://dx.doi.org/10.3233/RNN-140419}},
doi = {{10.3233/RNN-140419}},
volume = {{32}},
year = {{2014}},
}