Short-term grafting of human neural stem cells : Electrophysiological properties and motor behavioral amelioration in experimental Parkinson’s disease
Martínez-Serrano, Alberto ; Pereira, Marta P. ; Avaliani, Natalia ; Nelke, Anna ; Kokaia, Merab LU and Ramos-Moreno, Tania LU
(2016)
In Cell Transplantation
25(12).
p.2083-2097
- Abstract
Cell replacement therapy in Parkinson’s disease (PD) still lacks a study addressing the acquisition of electrophysiological properties of human grafted neural stem cells and their relation with the emergence of behavioral recovery after transplantation in the short term. Here we study the electrophysiological and biochemical profiles of two ventral mesencephalic human neural stem cell (NSC) clonal lines (C30-Bcl-XL and C32-Bcl-XL) that express high levels of Bcl-XL to enhance their neurogenic capacity, after grafting in an in vitro parkinsonian model. Electrophysiological recordings show that the majority of the cells derived from the transplants are not mature at 6 weeks after grafting, but 6.7% of the... (More)
Cell replacement therapy in Parkinson’s disease (PD) still lacks a study addressing the acquisition of electrophysiological properties of human grafted neural stem cells and their relation with the emergence of behavioral recovery after transplantation in the short term. Here we study the electrophysiological and biochemical profiles of two ventral mesencephalic human neural stem cell (NSC) clonal lines (C30-Bcl-XL and C32-Bcl-XL) that express high levels of Bcl-XL to enhance their neurogenic capacity, after grafting in an in vitro parkinsonian model. Electrophysiological recordings show that the majority of the cells derived from the transplants are not mature at 6 weeks after grafting, but 6.7% of the studied cells showed mature electrophysiological profiles. Nevertheless, parallel in vivo behavioral studies showed a significant motor improvement at 7 weeks postgrafting in the animals receiving C30-Bcl-XL, the cell line producing the highest amount of TH+ cells. Present results show that, at this postgrafting time point, behavioral amelioration highly correlates with the spatial dispersion of the TH+ grafted cells in the caudate putamen. The spatial dispersion, along with a high number of dopaminergic-derived cells, is crucial for behavioral improvements. Our findings have implications for long-term standardization of stem cell-based approaches in Parkinson’s disease.
(Less)
- author
- Martínez-Serrano, Alberto
; Pereira, Marta P.
; Avaliani, Natalia
; Nelke, Anna
; Kokaia, Merab
LU
and Ramos-Moreno, Tania
LU
- organization
- publishing date
- 2016
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- A9-dopaminergic phenotype, Electrophysiology, Human neural stem cells (hNSCs), Parkinson’s disease (PD), Ventral mesencephalon
- in
- Cell Transplantation
- volume
- 25
- issue
- 12
- pages
- 15 pages
- publisher
- Cognizant Communication Corporation
- external identifiers
-
- scopus:85007071959
- pmid:27324617
- wos:000390183200001
- ISSN
- 0963-6897
- DOI
- 10.3727/096368916X692069
- language
- English
- LU publication?
- yes
- id
- c46a774a-bda8-467f-9c5f-b526cd8e2ad1
- date added to LUP
- 2017-01-20 13:10:44
- date last changed
- 2024-03-07 20:42:18
@article{c46a774a-bda8-467f-9c5f-b526cd8e2ad1,
abstract = {{<p>Cell replacement therapy in Parkinson’s disease (PD) still lacks a study addressing the acquisition of electrophysiological properties of human grafted neural stem cells and their relation with the emergence of behavioral recovery after transplantation in the short term. Here we study the electrophysiological and biochemical profiles of two ventral mesencephalic human neural stem cell (NSC) clonal lines (C30-Bcl-X<sub>L</sub> and C32-Bcl-X<sub>L</sub>) that express high levels of Bcl-X<sub>L</sub> to enhance their neurogenic capacity, after grafting in an in vitro parkinsonian model. Electrophysiological recordings show that the majority of the cells derived from the transplants are not mature at 6 weeks after grafting, but 6.7% of the studied cells showed mature electrophysiological profiles. Nevertheless, parallel in vivo behavioral studies showed a significant motor improvement at 7 weeks postgrafting in the animals receiving C30-Bcl-X<sub>L</sub>, the cell line producing the highest amount of TH<sup>+</sup> cells. Present results show that, at this postgrafting time point, behavioral amelioration highly correlates with the spatial dispersion of the TH<sup>+</sup> grafted cells in the caudate putamen. The spatial dispersion, along with a high number of dopaminergic-derived cells, is crucial for behavioral improvements. Our findings have implications for long-term standardization of stem cell-based approaches in Parkinson’s disease.</p>}},
author = {{Martínez-Serrano, Alberto and Pereira, Marta P. and Avaliani, Natalia and Nelke, Anna and Kokaia, Merab and Ramos-Moreno, Tania}},
issn = {{0963-6897}},
keywords = {{A9-dopaminergic phenotype; Electrophysiology; Human neural stem cells (hNSCs); Parkinson’s disease (PD); Ventral mesencephalon}},
language = {{eng}},
number = {{12}},
pages = {{2083--2097}},
publisher = {{Cognizant Communication Corporation}},
series = {{Cell Transplantation}},
title = {{Short-term grafting of human neural stem cells : Electrophysiological properties and motor behavioral amelioration in experimental Parkinson’s disease}},
url = {{http://dx.doi.org/10.3727/096368916X692069}},
doi = {{10.3727/096368916X692069}},
volume = {{25}},
year = {{2016}},
}