The use of a recombinant lentiviral vector for ex vivo gene transfer into the rat CNS
Englund, Ulrica LU ; Ericson, Cecilia LU ; Rosenblad, Carl LU ; Mandel, Ronald J. ; Trono, Didier ; Wictorin, Klas LU and Lundberg, Cecilia LU
(2000)
In NeuroReport
11(18).
p.3973-3977
- Abstract
A major obstacle in ex vivo gene transfer has been the loss of transgene expression soon after implantation of the grafted transduced cells. Recently, a lentiviral vector system has been developed which has proven to express high levels of transgenes in vivo after direct injection into the tissue. In this study, we have investigated the use of such a vector for ex vivo gene transfer to the brain. A number of neural cell types were found to be permissive to transduction by the lentiviral vector in vitro and a majority of them expressed the transgene after transplantation to the rat brain. Transgene expression was detected up to 8 weeks post-grafting. These findings suggest that recombinant lentiviral vectors may be used for further... (More)
A major obstacle in ex vivo gene transfer has been the loss of transgene expression soon after implantation of the grafted transduced cells. Recently, a lentiviral vector system has been developed which has proven to express high levels of transgenes in vivo after direct injection into the tissue. In this study, we have investigated the use of such a vector for ex vivo gene transfer to the brain. A number of neural cell types were found to be permissive to transduction by the lentiviral vector in vitro and a majority of them expressed the transgene after transplantation to the rat brain. Transgene expression was detected up to 8 weeks post-grafting. These findings suggest that recombinant lentiviral vectors may be used for further development of ex vivo gene therapy protocols to the CNS.
(Less)
- author
- Englund, Ulrica
LU
; Ericson, Cecilia
LU
; Rosenblad, Carl
LU
; Mandel, Ronald J.
; Trono, Didier
; Wictorin, Klas
LU
and Lundberg, Cecilia
LU
- organization
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Ex vivo gene transfer, Green fluorescent protein, Lentiviral vector
- in
- NeuroReport
- volume
- 11
- issue
- 18
- pages
- 5 pages
- publisher
- Lippincott Williams & Wilkins
- external identifiers
-
- scopus:0034524787
- pmid:11192612
- ISSN
- 0959-4965
- language
- English
- LU publication?
- yes
- id
- c554bdf1-82f3-4f8f-bd29-0e1b5d4ac9bc
- date added to LUP
- 2016-11-14 12:22:17
- date last changed
- 2024-03-07 15:57:25
@article{c554bdf1-82f3-4f8f-bd29-0e1b5d4ac9bc,
abstract = {{<p>A major obstacle in ex vivo gene transfer has been the loss of transgene expression soon after implantation of the grafted transduced cells. Recently, a lentiviral vector system has been developed which has proven to express high levels of transgenes in vivo after direct injection into the tissue. In this study, we have investigated the use of such a vector for ex vivo gene transfer to the brain. A number of neural cell types were found to be permissive to transduction by the lentiviral vector in vitro and a majority of them expressed the transgene after transplantation to the rat brain. Transgene expression was detected up to 8 weeks post-grafting. These findings suggest that recombinant lentiviral vectors may be used for further development of ex vivo gene therapy protocols to the CNS.</p>}},
author = {{Englund, Ulrica and Ericson, Cecilia and Rosenblad, Carl and Mandel, Ronald J. and Trono, Didier and Wictorin, Klas and Lundberg, Cecilia}},
issn = {{0959-4965}},
keywords = {{Ex vivo gene transfer; Green fluorescent protein; Lentiviral vector}},
language = {{eng}},
number = {{18}},
pages = {{3973--3977}},
publisher = {{Lippincott Williams & Wilkins}},
series = {{NeuroReport}},
title = {{The use of a recombinant lentiviral vector for ex vivo gene transfer into the rat CNS}},
volume = {{11}},
year = {{2000}},
}