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FGF family members differentially regulate maturation and proliferation of stem cell-derived astrocytes

Savchenko, Ekaterina LU ; Teku, Gabriel N. LU ; Boza-Serrano, Antonio LU ; Russ, Kaspar LU ; Berns, Manon LU ; Deierborg, Tomas LU ; Lamas, Nuno J. LU ; Wichterle, Hynek ; Rothstein, Jeffrey and Henderson, Christopher E. , et al. (2019) In Scientific Reports 9(1).
Abstract

The glutamate transporter 1 (GLT1) is upregulated during astrocyte development and maturation in vivo and is vital for astrocyte function. Yet it is expressed at low levels by most cultured astrocytes. We previously showed that maturation of human and mouse stem cell-derived astrocytes - including functional glutamate uptake - could be enhanced by fibroblast growth factor (FGF)1 or FGF2. Here, we examined the specificity and mechanism of action of FGF2 and other FGF family members, as well as neurotrophic and differentiation factors, on mouse embryonic stem cell-derived astrocytes. We found that some FGFs - including FGF2, strongly increased GLT1 expression and enhanced astrocyte proliferation, while others (FGF16 and FGF18) mainly... (More)

The glutamate transporter 1 (GLT1) is upregulated during astrocyte development and maturation in vivo and is vital for astrocyte function. Yet it is expressed at low levels by most cultured astrocytes. We previously showed that maturation of human and mouse stem cell-derived astrocytes - including functional glutamate uptake - could be enhanced by fibroblast growth factor (FGF)1 or FGF2. Here, we examined the specificity and mechanism of action of FGF2 and other FGF family members, as well as neurotrophic and differentiation factors, on mouse embryonic stem cell-derived astrocytes. We found that some FGFs - including FGF2, strongly increased GLT1 expression and enhanced astrocyte proliferation, while others (FGF16 and FGF18) mainly affected maturation. Interestingly, BMP4 increased astrocytic GFAP expression, and BMP4-treated astrocytes failed to promote the survival of motor neurons in vitro. Whole transcriptome analysis showed that FGF2 treatment regulated multiple genes linked to cell division, and that the mRNA encoding GLT1 was one of the most strongly upregulated of all astrocyte canonical markers. Since GLT1 is expressed at reduced levels in many neurodegenerative diseases, activation of this pathway is of potential therapeutic interest. Furthermore, treatment with FGFs provides a robust means for expansion of functionally mature stem cell-derived astrocytes for preclinical investigation.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
9
issue
1
article number
9610
publisher
Nature Publishing Group
external identifiers
  • pmid:31270389
  • scopus:85069268744
ISSN
2045-2322
DOI
10.1038/s41598-019-46110-1
language
English
LU publication?
yes
id
cdde0134-9109-4cc0-9a44-5982688db979
date added to LUP
2019-07-29 11:47:57
date last changed
2024-04-02 15:19:32
@article{cdde0134-9109-4cc0-9a44-5982688db979,
  abstract     = {{<p>The glutamate transporter 1 (GLT1) is upregulated during astrocyte development and maturation in vivo and is vital for astrocyte function. Yet it is expressed at low levels by most cultured astrocytes. We previously showed that maturation of human and mouse stem cell-derived astrocytes - including functional glutamate uptake - could be enhanced by fibroblast growth factor (FGF)1 or FGF2. Here, we examined the specificity and mechanism of action of FGF2 and other FGF family members, as well as neurotrophic and differentiation factors, on mouse embryonic stem cell-derived astrocytes. We found that some FGFs - including FGF2, strongly increased GLT1 expression and enhanced astrocyte proliferation, while others (FGF16 and FGF18) mainly affected maturation. Interestingly, BMP4 increased astrocytic GFAP expression, and BMP4-treated astrocytes failed to promote the survival of motor neurons in vitro. Whole transcriptome analysis showed that FGF2 treatment regulated multiple genes linked to cell division, and that the mRNA encoding GLT1 was one of the most strongly upregulated of all astrocyte canonical markers. Since GLT1 is expressed at reduced levels in many neurodegenerative diseases, activation of this pathway is of potential therapeutic interest. Furthermore, treatment with FGFs provides a robust means for expansion of functionally mature stem cell-derived astrocytes for preclinical investigation.</p>}},
  author       = {{Savchenko, Ekaterina and Teku, Gabriel N. and Boza-Serrano, Antonio and Russ, Kaspar and Berns, Manon and Deierborg, Tomas and Lamas, Nuno J. and Wichterle, Hynek and Rothstein, Jeffrey and Henderson, Christopher E. and Vihinen, Mauno and Roybon, Laurent}},
  issn         = {{2045-2322}},
  language     = {{eng}},
  month        = {{07}},
  number       = {{1}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Scientific Reports}},
  title        = {{FGF family members differentially regulate maturation and proliferation of stem cell-derived astrocytes}},
  url          = {{http://dx.doi.org/10.1038/s41598-019-46110-1}},
  doi          = {{10.1038/s41598-019-46110-1}},
  volume       = {{9}},
  year         = {{2019}},
}