Axonal growth in mesothelial chambers : effects of a proximal preconditioning lesion and/or predegeneration of the distal nerve stump
Dahlin, L B LU
; Danielsen, N
LU
; Ochi, M
and Lundborg, G
LU
(1988)
In Experimental Neurology
99(3).
p.63-655
- Abstract
Preformed, autologous mesothelial chambers were utilized to study axonal growth following selective predegeneration of the distal nerve stump and/or preconditioning of the proximal nerve stump. The left and/or right sciatic nerve of rats was exposed and transected in the thigh. Two weeks after transection, the left proximal nerve stump was cross-anastomosed with the right distal nerve stump by using a mesothelial chamber leaving a 15-mm gap between the two nerve stumps. Previous studies have shown that axonal overgrowth normally does not occur over this gap distance to the distal stump. Three months after cross-anastomosing, regeneration across the 15-mm gap was evaluated by muscle action potential recordings and light microscopical... (More)
Preformed, autologous mesothelial chambers were utilized to study axonal growth following selective predegeneration of the distal nerve stump and/or preconditioning of the proximal nerve stump. The left and/or right sciatic nerve of rats was exposed and transected in the thigh. Two weeks after transection, the left proximal nerve stump was cross-anastomosed with the right distal nerve stump by using a mesothelial chamber leaving a 15-mm gap between the two nerve stumps. Previous studies have shown that axonal overgrowth normally does not occur over this gap distance to the distal stump. Three months after cross-anastomosing, regeneration across the 15-mm gap was evaluated by muscle action potential recordings and light microscopical examination. In experiments in which a distal nerve stump was selectively degenerated and the proximal segment was freshly cut, axons had bridged the 15-mm gap in six of seven rats. When a proximal preconditioned nerve stump was matched with a freshly cut distal stump, axonal overgrowth occurred in only 4 of 10 experiments. In experiments including a proximal preconditioned nerve stump and a distal predegenerated stump, axons bridged the gap in 6 of 8 experiments. We concluded that a priming lesion, including manipulation with proximal and/or distal stump, enhances axonal growth in mesothelial chambers.
(Less)
- author
- Dahlin, L B
LU
; Danielsen, N
LU
; Ochi, M
and Lundborg, G
LU
- organization
- publishing date
- 1988-03
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Axons, Equipment and Supplies, Female, Nerve Regeneration, Rats, Sciatic Nerve, Silicones, Journal Article, Research Support, Non-U.S. Gov't
- in
- Experimental Neurology
- volume
- 99
- issue
- 3
- pages
- 63 - 655
- publisher
- Elsevier
- external identifiers
-
- scopus:0023930226
- pmid:3342848
- ISSN
- 0014-4886
- DOI
- 10.1016/0014-4886(88)90182-3
- language
- English
- LU publication?
- yes
- id
- d514af83-218a-41be-88ee-1dbd41d916e5
- date added to LUP
- 2017-10-13 14:06:48
- date last changed
- 2024-01-14 07:25:06
@article{d514af83-218a-41be-88ee-1dbd41d916e5,
abstract = {{<p>Preformed, autologous mesothelial chambers were utilized to study axonal growth following selective predegeneration of the distal nerve stump and/or preconditioning of the proximal nerve stump. The left and/or right sciatic nerve of rats was exposed and transected in the thigh. Two weeks after transection, the left proximal nerve stump was cross-anastomosed with the right distal nerve stump by using a mesothelial chamber leaving a 15-mm gap between the two nerve stumps. Previous studies have shown that axonal overgrowth normally does not occur over this gap distance to the distal stump. Three months after cross-anastomosing, regeneration across the 15-mm gap was evaluated by muscle action potential recordings and light microscopical examination. In experiments in which a distal nerve stump was selectively degenerated and the proximal segment was freshly cut, axons had bridged the 15-mm gap in six of seven rats. When a proximal preconditioned nerve stump was matched with a freshly cut distal stump, axonal overgrowth occurred in only 4 of 10 experiments. In experiments including a proximal preconditioned nerve stump and a distal predegenerated stump, axons bridged the gap in 6 of 8 experiments. We concluded that a priming lesion, including manipulation with proximal and/or distal stump, enhances axonal growth in mesothelial chambers.</p>}},
author = {{Dahlin, L B and Danielsen, N and Ochi, M and Lundborg, G}},
issn = {{0014-4886}},
keywords = {{Animals; Axons; Equipment and Supplies; Female; Nerve Regeneration; Rats; Sciatic Nerve; Silicones; Journal Article; Research Support, Non-U.S. Gov't}},
language = {{eng}},
number = {{3}},
pages = {{63--655}},
publisher = {{Elsevier}},
series = {{Experimental Neurology}},
title = {{Axonal growth in mesothelial chambers : effects of a proximal preconditioning lesion and/or predegeneration of the distal nerve stump}},
url = {{http://dx.doi.org/10.1016/0014-4886(88)90182-3}},
doi = {{10.1016/0014-4886(88)90182-3}},
volume = {{99}},
year = {{1988}},
}