Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow
Berclaz, Corinne ; Szlag, Daniel ; Nguyen, David ; Extermann, Jérôme ; Bouwens, Arno ; Marchand, Paul J. ; Nilsson, Julia LU ; Schmidt-Christensen, Anja LU
; Holmberg, Dan
LU
and Grapin-Botton, Anne
, et al.
(2016)
In Biomedical Optics Express
7(11).
p.4569-4580
- Abstract
In diabetes, pancreatic ß-cells play a key role. These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into the blood stream. The dense vascularization of islets of Langerhans is critical for maintaining a proper regulation of blood glucose homeostasis and is known to be affected from the early stage of diabetes. The deep localization of these islets inside the pancreas in the abdominal cavity renders their in vivo visualization a challenging task. A fast label-free imaging method with high spatial resolution is required to study the vascular network of islets of Langerhans. Based on these requirements, we developed a label-free and three-dimensional... (More)
In diabetes, pancreatic ß-cells play a key role. These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into the blood stream. The dense vascularization of islets of Langerhans is critical for maintaining a proper regulation of blood glucose homeostasis and is known to be affected from the early stage of diabetes. The deep localization of these islets inside the pancreas in the abdominal cavity renders their in vivo visualization a challenging task. A fast label-free imaging method with high spatial resolution is required to study the vascular network of islets of Langerhans. Based on these requirements, we developed a label-free and three-dimensional imaging method for observing islets of Langerhans using extended-focus Fourier domain Optical Coherence Microscopy (xfOCM). In addition to structural imaging, this system provides threedimensional vascular network imaging and dynamic blood flow information within islets of Langerhans. We propose our method to deepen the understanding of the interconnection between diabetes and the evolution of the islet vascular network.
(Less)
- author
- Berclaz, Corinne
; Szlag, Daniel
; Nguyen, David
; Extermann, Jérôme
; Bouwens, Arno
; Marchand, Paul J.
; Nilsson, Julia
LU
; Schmidt-Christensen, Anja
LU
; Holmberg, Dan
LU
and Grapin-Botton, Anne
, et al. (More)
- Berclaz, Corinne
; Szlag, Daniel
; Nguyen, David
; Extermann, Jérôme
; Bouwens, Arno
; Marchand, Paul J.
; Nilsson, Julia
LU
; Schmidt-Christensen, Anja
LU
; Holmberg, Dan
LU
; Grapin-Botton, Anne
and Lasser, Theo
(Less)
- organization
- publishing date
- 2016-11-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Biology, Flow diagnostics, Optical coherence tomography, Three-dimensional image acquisition
- in
- Biomedical Optics Express
- volume
- 7
- issue
- 11
- article number
- #273588
- pages
- 12 pages
- publisher
- Optical Society of America
- external identifiers
-
- scopus:84994545324
- pmid:27895996
- wos:000387557600018
- ISSN
- 2156-7085
- DOI
- 10.1364/BOE.7.004569
- project
- inflammatory events leading to autoimmune Diabetes
- 3D imaging of inflammation and autoimmunity
- language
- English
- LU publication?
- yes
- id
- d751fdd0-f68e-4094-b032-f9d5ba1bd706
- date added to LUP
- 2016-12-05 13:37:57
- date last changed
- 2024-02-03 05:50:45
@article{d751fdd0-f68e-4094-b032-f9d5ba1bd706,
abstract = {{<p>In diabetes, pancreatic ß-cells play a key role. These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into the blood stream. The dense vascularization of islets of Langerhans is critical for maintaining a proper regulation of blood glucose homeostasis and is known to be affected from the early stage of diabetes. The deep localization of these islets inside the pancreas in the abdominal cavity renders their in vivo visualization a challenging task. A fast label-free imaging method with high spatial resolution is required to study the vascular network of islets of Langerhans. Based on these requirements, we developed a label-free and three-dimensional imaging method for observing islets of Langerhans using extended-focus Fourier domain Optical Coherence Microscopy (xfOCM). In addition to structural imaging, this system provides threedimensional vascular network imaging and dynamic blood flow information within islets of Langerhans. We propose our method to deepen the understanding of the interconnection between diabetes and the evolution of the islet vascular network.</p>}},
author = {{Berclaz, Corinne and Szlag, Daniel and Nguyen, David and Extermann, Jérôme and Bouwens, Arno and Marchand, Paul J. and Nilsson, Julia and Schmidt-Christensen, Anja and Holmberg, Dan and Grapin-Botton, Anne and Lasser, Theo}},
issn = {{2156-7085}},
keywords = {{Biology; Flow diagnostics; Optical coherence tomography; Three-dimensional image acquisition}},
language = {{eng}},
month = {{11}},
number = {{11}},
pages = {{4569--4580}},
publisher = {{Optical Society of America}},
series = {{Biomedical Optics Express}},
title = {{Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow}},
url = {{http://dx.doi.org/10.1364/BOE.7.004569}},
doi = {{10.1364/BOE.7.004569}},
volume = {{7}},
year = {{2016}},
}