Antifungal activities of secondary metabolites isolated from liquid fermentations of Stereum hirsutum (Sh134-11) against Botrytis cinerea (grey mould agent)
(2017) In Food and Chemical Toxicology 109. p.1048-1054- Abstract
Extracts obtained from liquid mycelial fermentations of the Chilean fungus Stereum hirsutum (Sh134-11) showed antifungal activity against Botrytis cinerea. Two types of extracts were obtained: EtOAc-extract (liquid phase) and MeOH-extract (mycelial phase). Plate diffusion assay showed that EtOAc-extracts were more active than MeOH-extracts. A large-scale fermentation of Sh134-11 and chromatographic methods allowed to isolated four compounds: MS-3, Vibralactone, Vibralactone B and Sterenin D. Only Sterenin D showed antifungal activity against B. cinerea in the tests performed. Effects on the mycelial growth of B. cinerea showed that Sterenin D showed inhibition at 1000-2000 μg/mL reaching 67% and 76% respectively. Sterenin D was more... (More)
Extracts obtained from liquid mycelial fermentations of the Chilean fungus Stereum hirsutum (Sh134-11) showed antifungal activity against Botrytis cinerea. Two types of extracts were obtained: EtOAc-extract (liquid phase) and MeOH-extract (mycelial phase). Plate diffusion assay showed that EtOAc-extracts were more active than MeOH-extracts. A large-scale fermentation of Sh134-11 and chromatographic methods allowed to isolated four compounds: MS-3, Vibralactone, Vibralactone B and Sterenin D. Only Sterenin D showed antifungal activity against B. cinerea in the tests performed. Effects on the mycelial growth of B. cinerea showed that Sterenin D showed inhibition at 1000-2000 μg/mL reaching 67% and 76% respectively. Sterenin D was more effective to control the sporogenesis, inhibiting in 96% the sporulation at 500 μg/mL. Assays showed that Sterenin D exhibited a minimal fungicidal concentration (MFC) of 50 μg/mL and minimal inhibitory concentration (MIC) at 20 μg/mL.Our study indicated that submerged fermentations of Chilean S. hirsutum (Sh134-11) produced extracts with antifungal activity and Sterenin D is responsible for this activity, which could be used as possible biofungicides alternative to synthetic fungicides.
(Less)
- author
- Aqueveque, Pedro ; Céspedes, Carlos L. ; Becerra, José ; Aranda, Mario and Sterner, Olov LU
- organization
- publishing date
- 2017-11
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Antifungal, Botrytis, Chilean fungi, Sterenin D, Stereum, Submerged cultures
- in
- Food and Chemical Toxicology
- volume
- 109
- pages
- 1048 - 1054
- publisher
- Elsevier
- external identifiers
-
- scopus:85019552054
- pmid:28528973
- wos:000415911500027
- ISSN
- 0278-6915
- DOI
- 10.1016/j.fct.2017.05.036
- language
- English
- LU publication?
- yes
- id
- e53aea91-28dd-4e6c-b5fb-350eccb23a72
- date added to LUP
- 2017-06-13 13:04:43
- date last changed
- 2024-04-14 13:07:28
@article{e53aea91-28dd-4e6c-b5fb-350eccb23a72,
abstract = {{<p>Extracts obtained from liquid mycelial fermentations of the Chilean fungus Stereum hirsutum (Sh134-11) showed antifungal activity against Botrytis cinerea. Two types of extracts were obtained: EtOAc-extract (liquid phase) and MeOH-extract (mycelial phase). Plate diffusion assay showed that EtOAc-extracts were more active than MeOH-extracts. A large-scale fermentation of Sh134-11 and chromatographic methods allowed to isolated four compounds: MS-3, Vibralactone, Vibralactone B and Sterenin D. Only Sterenin D showed antifungal activity against B. cinerea in the tests performed. Effects on the mycelial growth of B. cinerea showed that Sterenin D showed inhibition at 1000-2000 μg/mL reaching 67% and 76% respectively. Sterenin D was more effective to control the sporogenesis, inhibiting in 96% the sporulation at 500 μg/mL. Assays showed that Sterenin D exhibited a minimal fungicidal concentration (MFC) of 50 μg/mL and minimal inhibitory concentration (MIC) at 20 μg/mL.Our study indicated that submerged fermentations of Chilean S. hirsutum (Sh134-11) produced extracts with antifungal activity and Sterenin D is responsible for this activity, which could be used as possible biofungicides alternative to synthetic fungicides.</p>}},
author = {{Aqueveque, Pedro and Céspedes, Carlos L. and Becerra, José and Aranda, Mario and Sterner, Olov}},
issn = {{0278-6915}},
keywords = {{Antifungal; Botrytis; Chilean fungi; Sterenin D; Stereum; Submerged cultures}},
language = {{eng}},
pages = {{1048--1054}},
publisher = {{Elsevier}},
series = {{Food and Chemical Toxicology}},
title = {{Antifungal activities of secondary metabolites isolated from liquid fermentations of Stereum hirsutum (Sh134-11) against Botrytis cinerea (grey mould agent)}},
url = {{http://dx.doi.org/10.1016/j.fct.2017.05.036}},
doi = {{10.1016/j.fct.2017.05.036}},
volume = {{109}},
year = {{2017}},
}