Signal enhancement in supercritical fluid chromatography-diode-array detection with multiple injection
(2019) In Journal of Separation Science 42(24). p.3727-3737- Abstract
To circumvent the detrimental effects of large-volume injection with fixed-loop injector in modern supercritical fluid chromatography, the feasibility of performing multiple injection was investigated. By accumulating analytes from a certain number of continual small-volume injections, compounds can be concentrated on the column head, and this leads to signal enhancement compared with a single injection. The signal to noise enhancement of different compounds appeared to be associated with their retention on different stationary phases and with type of sample diluent. The diethylamine column gave the best signal to noise enhancement when acetonitrile was used as sample diluent and the 2-picolylamine column showed the best overall... (More)
To circumvent the detrimental effects of large-volume injection with fixed-loop injector in modern supercritical fluid chromatography, the feasibility of performing multiple injection was investigated. By accumulating analytes from a certain number of continual small-volume injections, compounds can be concentrated on the column head, and this leads to signal enhancement compared with a single injection. The signal to noise enhancement of different compounds appeared to be associated with their retention on different stationary phases and with type of sample diluent. The diethylamine column gave the best signal to noise enhancement when acetonitrile was used as sample diluent and the 2-picolylamine column showed the best overall performance with water as the sample diluent. The advantage of multiple injection over one-time large-volume injection was proven with sulfanilamide, with both acetonitrile and water as sample diluents. The multiple injection approach exhibited comparable within- and between-day precision of retention time and peak area with those of single injections. The potential of the multiple injection approach was demonstrated in the analysis of sulfanilamide-spiked honey extract and diclofenac-spiked ground water sample. The limitations of this approach were also discussed.
(Less)
- author
- Sun, Mingzhe LU ; Turner, Charlotta LU and Sandahl, Margareta LU
- organization
- publishing date
- 2019-10-17
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- large injection volume, multiple injection, signal enhancement, supercritical fluid chromatography
- in
- Journal of Separation Science
- volume
- 42
- issue
- 24
- pages
- 3727 - 3737
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:31625267
- scopus:85075524824
- ISSN
- 1615-9306
- DOI
- 10.1002/jssc.201900614
- language
- English
- LU publication?
- yes
- id
- e82f5dbc-c710-48de-a567-4886d3f35db8
- date added to LUP
- 2019-12-09 10:14:52
- date last changed
- 2024-03-04 09:45:12
@article{e82f5dbc-c710-48de-a567-4886d3f35db8,
abstract = {{<p>To circumvent the detrimental effects of large-volume injection with fixed-loop injector in modern supercritical fluid chromatography, the feasibility of performing multiple injection was investigated. By accumulating analytes from a certain number of continual small-volume injections, compounds can be concentrated on the column head, and this leads to signal enhancement compared with a single injection. The signal to noise enhancement of different compounds appeared to be associated with their retention on different stationary phases and with type of sample diluent. The diethylamine column gave the best signal to noise enhancement when acetonitrile was used as sample diluent and the 2-picolylamine column showed the best overall performance with water as the sample diluent. The advantage of multiple injection over one-time large-volume injection was proven with sulfanilamide, with both acetonitrile and water as sample diluents. The multiple injection approach exhibited comparable within- and between-day precision of retention time and peak area with those of single injections. The potential of the multiple injection approach was demonstrated in the analysis of sulfanilamide-spiked honey extract and diclofenac-spiked ground water sample. The limitations of this approach were also discussed.</p>}},
author = {{Sun, Mingzhe and Turner, Charlotta and Sandahl, Margareta}},
issn = {{1615-9306}},
keywords = {{large injection volume; multiple injection; signal enhancement; supercritical fluid chromatography}},
language = {{eng}},
month = {{10}},
number = {{24}},
pages = {{3727--3737}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Journal of Separation Science}},
title = {{Signal enhancement in supercritical fluid chromatography-diode-array detection with multiple injection}},
url = {{http://dx.doi.org/10.1002/jssc.201900614}},
doi = {{10.1002/jssc.201900614}},
volume = {{42}},
year = {{2019}},
}