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6-Hydroxydopamine induced degeneration of noradrenaline neurons in the scorbutic guinea-pig

Jonsson, Gösta ; Lohmander, Stefan LU orcid and Sachs, Charlotte (1974) In Biochemical Pharmacology 23(18). p.2585-2593
Abstract

The effect of the neurotoxic compound 6-hydroxydopamine and its immediate precursor 6-hydroxy-DOPA on noradrenaline uptake and storage in central and peripheral catecholamine neurons of scorbutic and normal guinea-pigs has been investigated. Endogenous noradrenaline in heart and brain as well as the in vitro uptake-accumulation of 3H-noradrenaline in iris and slices of heart and brain were not significantly changed in scorbutic animals. The in vitro formation of 3H-noradrenaline from 3H-dopamine was markedly reduced in heart slices of scorbutic guinea-pigs, due to ascorbic acid being a co-factor for dopamine-β-hydroxylase. There was an increased depletion of brain noradrenaline following tyrosine... (More)

The effect of the neurotoxic compound 6-hydroxydopamine and its immediate precursor 6-hydroxy-DOPA on noradrenaline uptake and storage in central and peripheral catecholamine neurons of scorbutic and normal guinea-pigs has been investigated. Endogenous noradrenaline in heart and brain as well as the in vitro uptake-accumulation of 3H-noradrenaline in iris and slices of heart and brain were not significantly changed in scorbutic animals. The in vitro formation of 3H-noradrenaline from 3H-dopamine was markedly reduced in heart slices of scorbutic guinea-pigs, due to ascorbic acid being a co-factor for dopamine-β-hydroxylase. There was an increased depletion of brain noradrenaline following tyrosine hydroxylase inhibition produced by α-methyl-p-tyrosine methylester in scorbutic animals, indicating an increased NA turnover. Administration of 6-hydroxydopamine or 6-hydroxy-DOPA resulted in a similar reduction of endogenous NA in brain and heart as well as of the in vitro uptake of 3H-noradrenaline in iris, and slices from heart, cerebral cortex and hypothalamus in scorbutic and control guinea-pigs. These results are discussed in view of current hypotheses on mechanisms involved in the neurotoxic action of 6-hydroxydopamine on catecholamine neurons.

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publication status
published
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Biochemical Pharmacology
volume
23
issue
18
pages
9 pages
publisher
Elsevier
external identifiers
  • pmid:4153539
  • scopus:0016215994
ISSN
0006-2952
DOI
10.1016/0006-2952(74)90181-6
language
English
LU publication?
yes
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f01a0071-9e58-4950-ba28-3419ecac307c
date added to LUP
2016-05-04 12:38:56
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2024-01-04 02:37:31
@article{f01a0071-9e58-4950-ba28-3419ecac307c,
  abstract     = {{<p>The effect of the neurotoxic compound 6-hydroxydopamine and its immediate precursor 6-hydroxy-DOPA on noradrenaline uptake and storage in central and peripheral catecholamine neurons of scorbutic and normal guinea-pigs has been investigated. Endogenous noradrenaline in heart and brain as well as the in vitro uptake-accumulation of <sup>3</sup>H-noradrenaline in iris and slices of heart and brain were not significantly changed in scorbutic animals. The in vitro formation of <sup>3</sup>H-noradrenaline from <sup>3</sup>H-dopamine was markedly reduced in heart slices of scorbutic guinea-pigs, due to ascorbic acid being a co-factor for dopamine-β-hydroxylase. There was an increased depletion of brain noradrenaline following tyrosine hydroxylase inhibition produced by α-methyl-p-tyrosine methylester in scorbutic animals, indicating an increased NA turnover. Administration of 6-hydroxydopamine or 6-hydroxy-DOPA resulted in a similar reduction of endogenous NA in brain and heart as well as of the in vitro uptake of <sup>3</sup>H-noradrenaline in iris, and slices from heart, cerebral cortex and hypothalamus in scorbutic and control guinea-pigs. These results are discussed in view of current hypotheses on mechanisms involved in the neurotoxic action of 6-hydroxydopamine on catecholamine neurons.</p>}},
  author       = {{Jonsson, Gösta and Lohmander, Stefan and Sachs, Charlotte}},
  issn         = {{0006-2952}},
  language     = {{eng}},
  month        = {{09}},
  number       = {{18}},
  pages        = {{2585--2593}},
  publisher    = {{Elsevier}},
  series       = {{Biochemical Pharmacology}},
  title        = {{6-Hydroxydopamine induced degeneration of noradrenaline neurons in the scorbutic guinea-pig}},
  url          = {{http://dx.doi.org/10.1016/0006-2952(74)90181-6}},
  doi          = {{10.1016/0006-2952(74)90181-6}},
  volume       = {{23}},
  year         = {{1974}},
}