Carbon and Nitrogen Amendments Lead to Differential Growth of Bacterial and Fungal Communities in a High-pH Soil
(2018) In Pedosphere 28(2). p.255-260- Abstract
Microbial growth in soil is mostly limited by lack of carbon (C). However, adding fresh, C-rich litter can induce nitrogen (N) limitation. We studied the effect of alleviating C and N limitation in high-pH (> 8) soils, soils expected to favor bacterial over fungal growth. Nitrogen limitation was induced by incubating soils amended with C-rich substrate (starch or straw) for 4 weeks. Limiting nutrients and the effects of alleviating limitation were then studied by adding C (as glucose) or N (as NH4NO3) and measuring microbial growth and respiration after 4 d. In non-amended, C-limited soils, adding C but not N increased both microbial respiration and bacterial growth. In N-limited, substrate-amended soils, adding... (More)
Microbial growth in soil is mostly limited by lack of carbon (C). However, adding fresh, C-rich litter can induce nitrogen (N) limitation. We studied the effect of alleviating C and N limitation in high-pH (> 8) soils, soils expected to favor bacterial over fungal growth. Nitrogen limitation was induced by incubating soils amended with C-rich substrate (starch or straw) for 4 weeks. Limiting nutrients and the effects of alleviating limitation were then studied by adding C (as glucose) or N (as NH4NO3) and measuring microbial growth and respiration after 4 d. In non-amended, C-limited soils, adding C but not N increased both microbial respiration and bacterial growth. In N-limited, substrate-amended soils, adding C increased respiration, whereas adding N increased both microbial respiration and growth. Inducing N limitation by amending with straw was most easily detected in increased fungal growth after the addition of N, whereas with starch, only bacterial growth responded to alleviating N limitation. Compared to earlier results using a low-pH soil, the effect of substrate used to induce N limitation was more important than pH for inducing bacterial or fungal growth after alleviating N limitation. Furthermore, we found no evidence that alleviating N limitation resulted in decreased respiration concomitant with increased microbial growth in soil, suggesting no drastic changes in C use efficiency.
(Less)
- author
- KAMBLE, Pramod N. LU and BÅÅTH, Erland LU
- organization
- publishing date
- 2018-04-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- acetate incorporation into ergosterol, carbon use efficiency, leucine incorporation, limiting nutrient, microbial growth, microbial respiration, N limitation
- in
- Pedosphere
- volume
- 28
- issue
- 2
- pages
- 6 pages
- publisher
- Institute of Soil Science
- external identifiers
-
- scopus:85046809560
- ISSN
- 1002-0160
- DOI
- 10.1016/S1002-0160(18)60014-1
- language
- English
- LU publication?
- yes
- id
- f64d3afe-775d-4ed7-b00c-d85910d8a690
- date added to LUP
- 2018-05-24 15:41:49
- date last changed
- 2022-03-17 07:42:04
@article{f64d3afe-775d-4ed7-b00c-d85910d8a690, abstract = {{<p>Microbial growth in soil is mostly limited by lack of carbon (C). However, adding fresh, C-rich litter can induce nitrogen (N) limitation. We studied the effect of alleviating C and N limitation in high-pH (> 8) soils, soils expected to favor bacterial over fungal growth. Nitrogen limitation was induced by incubating soils amended with C-rich substrate (starch or straw) for 4 weeks. Limiting nutrients and the effects of alleviating limitation were then studied by adding C (as glucose) or N (as NH<sub>4</sub>NO<sub>3</sub>) and measuring microbial growth and respiration after 4 d. In non-amended, C-limited soils, adding C but not N increased both microbial respiration and bacterial growth. In N-limited, substrate-amended soils, adding C increased respiration, whereas adding N increased both microbial respiration and growth. Inducing N limitation by amending with straw was most easily detected in increased fungal growth after the addition of N, whereas with starch, only bacterial growth responded to alleviating N limitation. Compared to earlier results using a low-pH soil, the effect of substrate used to induce N limitation was more important than pH for inducing bacterial or fungal growth after alleviating N limitation. Furthermore, we found no evidence that alleviating N limitation resulted in decreased respiration concomitant with increased microbial growth in soil, suggesting no drastic changes in C use efficiency.</p>}}, author = {{KAMBLE, Pramod N. and BÅÅTH, Erland}}, issn = {{1002-0160}}, keywords = {{acetate incorporation into ergosterol; carbon use efficiency; leucine incorporation; limiting nutrient; microbial growth; microbial respiration; N limitation}}, language = {{eng}}, month = {{04}}, number = {{2}}, pages = {{255--260}}, publisher = {{Institute of Soil Science}}, series = {{Pedosphere}}, title = {{Carbon and Nitrogen Amendments Lead to Differential Growth of Bacterial and Fungal Communities in a High-pH Soil}}, url = {{http://dx.doi.org/10.1016/S1002-0160(18)60014-1}}, doi = {{10.1016/S1002-0160(18)60014-1}}, volume = {{28}}, year = {{2018}}, }