Microcontact imprinted plasmonic nanosensors : Powerful tools in the detection of salmonella paratyphi
(2017) In Sensors 17(6).- Abstract
Identification of pathogenic microorganisms by traditional methods is slow and cumbersome. Therefore, the focus today is on developing new and quicker analytical methods. In this study, a Surface Plasmon Resonance (SPR) sensor with a microcontact imprinted sensor chip was developed for detecting Salmonella paratyphi. For this purpose, the stamps of the target microorganism were prepared and then, microcontact S. paratyphi-imprinted SPR chips were prepared with the functional monomer N-methacryloyl-L-histidine methyl ester (MAH). Characterization studies of the SPR chips were carried out with ellipsometry and scanning electron microscopy (SEM). The real-time Salmonella paratyphi detection was performed within the range of 2.5 ×... (More)
Identification of pathogenic microorganisms by traditional methods is slow and cumbersome. Therefore, the focus today is on developing new and quicker analytical methods. In this study, a Surface Plasmon Resonance (SPR) sensor with a microcontact imprinted sensor chip was developed for detecting Salmonella paratyphi. For this purpose, the stamps of the target microorganism were prepared and then, microcontact S. paratyphi-imprinted SPR chips were prepared with the functional monomer N-methacryloyl-L-histidine methyl ester (MAH). Characterization studies of the SPR chips were carried out with ellipsometry and scanning electron microscopy (SEM). The real-time Salmonella paratyphi detection was performed within the range of 2.5 × 106-15 × 106 CFU/mL. Selectivity of the prepared sensors was examined by using competing bacterial strains such as Escherichia coli, Staphylococcus aureus and Bacillus subtilis. The imprinting efficiency of the prepared sensor system was determined by evaluating the responses of the SPR chips prepared with both molecularly imprinted polymers (MIPs) and non-imprinted polymers (NIPs). Real sample experiments were performed with apple juice. The recognition of Salmonella paratyphi was achieved using these SPR sensor with a detection limit of 1.4 × 106 CFU/mL. In conclusion, SPR sensor has the potential to serve as an excellent candidate for monitoring Salmonella paratyphi in food supplies or contaminated water and clearly makes it possible to develop rapid and appropriate control strategies.
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- author
- Perçin, Isık ; Idil, Neslihan ; Bakhshpour, Monireh ; Yılmaz, Erkut ; Mattiasson, Bo LU and Denizli, Adil
- organization
- publishing date
- 2017-06-13
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Microcontact imprinting, N-methacryloyl-L-histidine methyl ester, Salmonella paratyphi, SPR biosensor
- in
- Sensors
- volume
- 17
- issue
- 6
- article number
- 1375
- publisher
- MDPI AG
- external identifiers
-
- wos:000404553900191
- pmid:28608810
- scopus:85020943648
- ISSN
- 1424-8220
- DOI
- 10.3390/s17061375
- language
- English
- LU publication?
- yes
- id
- f7b15a0a-b833-4c52-95c0-6b4b8eb6b5a5
- date added to LUP
- 2017-07-04 09:31:52
- date last changed
- 2024-03-31 10:57:55
@article{f7b15a0a-b833-4c52-95c0-6b4b8eb6b5a5,
abstract = {{<p>Identification of pathogenic microorganisms by traditional methods is slow and cumbersome. Therefore, the focus today is on developing new and quicker analytical methods. In this study, a Surface Plasmon Resonance (SPR) sensor with a microcontact imprinted sensor chip was developed for detecting Salmonella paratyphi. For this purpose, the stamps of the target microorganism were prepared and then, microcontact S. paratyphi-imprinted SPR chips were prepared with the functional monomer N-methacryloyl-L-histidine methyl ester (MAH). Characterization studies of the SPR chips were carried out with ellipsometry and scanning electron microscopy (SEM). The real-time Salmonella paratyphi detection was performed within the range of 2.5 × 10<sup>6</sup>-15 × 10<sup>6</sup> CFU/mL. Selectivity of the prepared sensors was examined by using competing bacterial strains such as Escherichia coli, Staphylococcus aureus and Bacillus subtilis. The imprinting efficiency of the prepared sensor system was determined by evaluating the responses of the SPR chips prepared with both molecularly imprinted polymers (MIPs) and non-imprinted polymers (NIPs). Real sample experiments were performed with apple juice. The recognition of Salmonella paratyphi was achieved using these SPR sensor with a detection limit of 1.4 × 10<sup>6</sup> CFU/mL. In conclusion, SPR sensor has the potential to serve as an excellent candidate for monitoring Salmonella paratyphi in food supplies or contaminated water and clearly makes it possible to develop rapid and appropriate control strategies.</p>}},
author = {{Perçin, Isık and Idil, Neslihan and Bakhshpour, Monireh and Yılmaz, Erkut and Mattiasson, Bo and Denizli, Adil}},
issn = {{1424-8220}},
keywords = {{Microcontact imprinting; N-methacryloyl-L-histidine methyl ester; Salmonella paratyphi; SPR biosensor}},
language = {{eng}},
month = {{06}},
number = {{6}},
publisher = {{MDPI AG}},
series = {{Sensors}},
title = {{Microcontact imprinted plasmonic nanosensors : Powerful tools in the detection of salmonella paratyphi}},
url = {{http://dx.doi.org/10.3390/s17061375}},
doi = {{10.3390/s17061375}},
volume = {{17}},
year = {{2017}},
}