Analysis by high-performance liquid chromatography of radioactively labeled carbohydrate components of proteoglycans
Lohmander, Stefan LU
(1986)
In Analytical Biochemistry
154(1).
p.75-84
- Abstract
Methods were developed for the separation of radioactively labeled carbohydrate components of proteoglycans by isocratic ion-moderated partition HPLC. Neutral sugars were separated after hydrolysis in trifluoroacetic acid with baseline separation between glucose, xylose, galactose, fucose, and mannose. N-Acetylneuraminic acid, N-acetylated hexosamines, glucose, galactose, and xylitol were likewise well separated from each other under isocratic elution conditions. Glucuronic acid, iduronic acid, and their lactones were separated after hydrolysis in formic acid and sulfuric acid. Glucosamine, galactosamine, galactosaminitol, and glucosaminitol were separated by HPLC on a cation exchanger with neutral buffer after hydrolysis in... (More)
Methods were developed for the separation of radioactively labeled carbohydrate components of proteoglycans by isocratic ion-moderated partition HPLC. Neutral sugars were separated after hydrolysis in trifluoroacetic acid with baseline separation between glucose, xylose, galactose, fucose, and mannose. N-Acetylneuraminic acid, N-acetylated hexosamines, glucose, galactose, and xylitol were likewise well separated from each other under isocratic elution conditions. Glucuronic acid, iduronic acid, and their lactones were separated after hydrolysis in formic acid and sulfuric acid. Glucosamine, galactosamine, galactosaminitol, and glucosaminitol were separated by HPLC on a cation exchanger with neutral buffer after hydrolysis in hydrochloric acid. The separation techniques also proved useful in fractionation of exoglycosidase digests of O- and N-linked oligosaccharides. Separations of aldoses, hexosamines, and uronic acids were adapted to sensitive photometric detection.
(Less)
- author
- Lohmander, Stefan
LU
- organization
- publishing date
- 1986
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- glycoproteins, HPLC carbohydrates, liquid scintillation counting, polysaccharides, protein processing, sugar analysis
- in
- Analytical Biochemistry
- volume
- 154
- issue
- 1
- pages
- 10 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:3706739
- scopus:0022445512
- ISSN
- 0003-2697
- DOI
- 10.1016/0003-2697(86)90498-7
- language
- English
- LU publication?
- yes
- id
- fc90eeeb-55b3-4487-93ce-ac0e74c492bc
- date added to LUP
- 2016-05-04 12:59:00
- date last changed
- 2024-01-04 02:43:03
@article{fc90eeeb-55b3-4487-93ce-ac0e74c492bc,
abstract = {{<p>Methods were developed for the separation of radioactively labeled carbohydrate components of proteoglycans by isocratic ion-moderated partition HPLC. Neutral sugars were separated after hydrolysis in trifluoroacetic acid with baseline separation between glucose, xylose, galactose, fucose, and mannose. N-Acetylneuraminic acid, N-acetylated hexosamines, glucose, galactose, and xylitol were likewise well separated from each other under isocratic elution conditions. Glucuronic acid, iduronic acid, and their lactones were separated after hydrolysis in formic acid and sulfuric acid. Glucosamine, galactosamine, galactosaminitol, and glucosaminitol were separated by HPLC on a cation exchanger with neutral buffer after hydrolysis in hydrochloric acid. The separation techniques also proved useful in fractionation of exoglycosidase digests of O- and N-linked oligosaccharides. Separations of aldoses, hexosamines, and uronic acids were adapted to sensitive photometric detection.</p>}},
author = {{Lohmander, Stefan}},
issn = {{0003-2697}},
keywords = {{glycoproteins; HPLC carbohydrates; liquid scintillation counting; polysaccharides; protein processing; sugar analysis}},
language = {{eng}},
number = {{1}},
pages = {{75--84}},
publisher = {{Elsevier}},
series = {{Analytical Biochemistry}},
title = {{Analysis by high-performance liquid chromatography of radioactively labeled carbohydrate components of proteoglycans}},
url = {{http://dx.doi.org/10.1016/0003-2697(86)90498-7}},
doi = {{10.1016/0003-2697(86)90498-7}},
volume = {{154}},
year = {{1986}},
}