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Distribution of GABA immunoreactivity in kainic acid-treated rabbit retina

Perez, M T LU and Davanger, S (1994) In Experimental Brain Research 100(2). p.38-227
Abstract

Ischaemic retinal cell degeneration seems to involve both NMDA and non-NMDA receptor over-stimulation. However, different retinal cell types differ largely in their susceptibility to excitatory amino acid-induced neurotoxicity. We have investigated the vulnerability of GABAergic cells in the rabbit retina to the non-NMDA receptor agonist kainic acid (KA). The distribution of GABA immunoreactivity (GABA-IR) was examined in the central inferior retina at different survival times (5 h-6 days) following an intra-ocular injection of 140 nmol KA and compared to that of control and untreated retinas. In the normal retina, the majority of GABA-positive cells (79%) were located in the inner nuclear layer (INL), in one to four cell rows next to... (More)

Ischaemic retinal cell degeneration seems to involve both NMDA and non-NMDA receptor over-stimulation. However, different retinal cell types differ largely in their susceptibility to excitatory amino acid-induced neurotoxicity. We have investigated the vulnerability of GABAergic cells in the rabbit retina to the non-NMDA receptor agonist kainic acid (KA). The distribution of GABA immunoreactivity (GABA-IR) was examined in the central inferior retina at different survival times (5 h-6 days) following an intra-ocular injection of 140 nmol KA and compared to that of control and untreated retinas. In the normal retina, the majority of GABA-positive cells (79%) were located in the inner nuclear layer (INL), in one to four cell rows next to the inner plexiform layer (IPL), and in one cell row next to the outer plexiform layer (OPL). The remainder (21%) were found in the ganglion cell layer (GCL). Dense immunoreactivity was seen throughout the IPL. In the OPL, stained dots and occasional immunoreactive large processes could be seen. KA-exposed retinas processed for GABA immunocytochemistry 5 and 24 h after the injection showed an 85% reduction in the number of GABA immunoreactive cells. About the same degree of depletion was seen among GABA-IR cells located at different retinal levels. However, at these survival times, immunostaining was observed in three distinct bands in the IPL, indicating that the vulnerability to KA is not uniformly distributed among all GABAergic cells. At 48 h, an additional decrease in the number of labelled cells was noted, but immunoreactive cells were still found both in the INL and GCL. Even 6 days after KA treatment, a few stained cell bodies were seen in the INL next to the IPL, as well as a few processes in the IPL. The study shows that KA receptor overstimulation induces a marked depletion of the endogenous cellular GABA pools of the central rabbit retina, most likely as a result of GABAergic cell loss. However, a small population of GABAergic cells located in the INL appears to be less vulnerable to the toxic effects of 140 nmol KA.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Immunohistochemistry, Kainic Acid, Neurons, Rabbits, Retina, Retinal Ganglion Cells, gamma-Aminobutyric Acid, Journal Article, Research Support, Non-U.S. Gov't
in
Experimental Brain Research
volume
100
issue
2
pages
38 - 227
publisher
Springer
external identifiers
  • Scopus:0028122361
ISSN
0014-4819
DOI
10.1007/BF00227193
language
English
LU publication?
no
id
0cfc3e9f-f5eb-4707-9574-e0f6bbcd4e4f
date added to LUP
2016-10-27 19:34:51
date last changed
2016-11-22 14:12:26
@misc{0cfc3e9f-f5eb-4707-9574-e0f6bbcd4e4f,
  abstract     = {<p>Ischaemic retinal cell degeneration seems to involve both NMDA and non-NMDA receptor over-stimulation. However, different retinal cell types differ largely in their susceptibility to excitatory amino acid-induced neurotoxicity. We have investigated the vulnerability of GABAergic cells in the rabbit retina to the non-NMDA receptor agonist kainic acid (KA). The distribution of GABA immunoreactivity (GABA-IR) was examined in the central inferior retina at different survival times (5 h-6 days) following an intra-ocular injection of 140 nmol KA and compared to that of control and untreated retinas. In the normal retina, the majority of GABA-positive cells (79%) were located in the inner nuclear layer (INL), in one to four cell rows next to the inner plexiform layer (IPL), and in one cell row next to the outer plexiform layer (OPL). The remainder (21%) were found in the ganglion cell layer (GCL). Dense immunoreactivity was seen throughout the IPL. In the OPL, stained dots and occasional immunoreactive large processes could be seen. KA-exposed retinas processed for GABA immunocytochemistry 5 and 24 h after the injection showed an 85% reduction in the number of GABA immunoreactive cells. About the same degree of depletion was seen among GABA-IR cells located at different retinal levels. However, at these survival times, immunostaining was observed in three distinct bands in the IPL, indicating that the vulnerability to KA is not uniformly distributed among all GABAergic cells. At 48 h, an additional decrease in the number of labelled cells was noted, but immunoreactive cells were still found both in the INL and GCL. Even 6 days after KA treatment, a few stained cell bodies were seen in the INL next to the IPL, as well as a few processes in the IPL. The study shows that KA receptor overstimulation induces a marked depletion of the endogenous cellular GABA pools of the central rabbit retina, most likely as a result of GABAergic cell loss. However, a small population of GABAergic cells located in the INL appears to be less vulnerable to the toxic effects of 140 nmol KA.</p>},
  author       = {Perez, M T and Davanger, S},
  issn         = {0014-4819},
  keyword      = {Animals,Immunohistochemistry,Kainic Acid,Neurons,Rabbits,Retina,Retinal Ganglion Cells,gamma-Aminobutyric Acid,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {2},
  pages        = {38--227},
  publisher    = {ARRAY(0xb892180)},
  series       = {Experimental Brain Research},
  title        = {Distribution of GABA immunoreactivity in kainic acid-treated rabbit retina},
  url          = {http://dx.doi.org/ 10.1007/BF00227193},
  volume       = {100},
  year         = {1994},
}