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Intracerebral grafting of neuronal cell suspensions. VII. Recovery of choline acetyltransferase activity and acetylcholine synthesis in the denervated hippccampus reinnervated by septal suspension implants

Björklund, Anders LU ; Gage, F H; Schmidt, R H; Stenevi, U and Dunnett, S B (1983) In Acta Physiol.Scand. Suppl. 522. p.59-66
Abstract
The time-course and magnitude of fibre outgrowth from septal suspension grafts injected into the previously denervated hippocampal formation was monitored by measurements of choline acetyltransferase (ChAT), and the activity of the grafted neurons was assessed by measurements of [14C]acetylcholine (ACh) synthesis from [14C]glucose in vitro. Graft-derived ChAT activity was barely detectable 10 days after grafting, but increased sharply between 10 days and 1 month in the areas of the hippocampus located close to the septal implants. By 6 months ChAT activity was restored to near normal levels in all segments of the previously denervated hippocampus. The overall hippocampal [14C]ACh synthesis was also restored to normal levels in the grafted... (More)
The time-course and magnitude of fibre outgrowth from septal suspension grafts injected into the previously denervated hippocampal formation was monitored by measurements of choline acetyltransferase (ChAT), and the activity of the grafted neurons was assessed by measurements of [14C]acetylcholine (ACh) synthesis from [14C]glucose in vitro. Graft-derived ChAT activity was barely detectable 10 days after grafting, but increased sharply between 10 days and 1 month in the areas of the hippocampus located close to the septal implants. By 6 months ChAT activity was restored to near normal levels in all segments of the previously denervated hippocampus. The overall hippocampal [14C]ACh synthesis was also restored to normal levels in the grafted animals, and estimates of the ACh turnover rate suggested that the transmitter machinery of the newly established "septo-hippocampal" connections operated at a rate similar to that of the intrinsic septohippocampal pathway. The intrahippocampal septal suspension grafts, similar to the intrastriatal nigral grafts, thus seem to be capable of maintaining function at a relatively "physiological" level despite their abnormal positions. (Less)
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keywords
Isogeneic Acetylcholine Choline O-Acetyltransferase, Acetylcholine/biosynthesis Animals Choline O-Acetyltransferase/metabolism* Embryo, Inbred Strains Septum Pellucidum/transplantation* Time Factors Transplantation, Mammalian/cytology Female Hippocampus/metabolism Hippocampus/surgery Neurons/metabolism Neurons/transplantation* Rats Rats
in
Acta Physiol.Scand.
volume
Suppl. 522
pages
59 - 66
publisher
Wiley-Blackwell
language
English
LU publication?
yes
id
582a90f8-5057-458d-9dd2-6bd450346143 (old id 1030589)
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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=6586056dopt=Abstract
date added to LUP
2008-02-11 13:30:00
date last changed
2016-04-16 10:58:50
@misc{582a90f8-5057-458d-9dd2-6bd450346143,
  abstract     = {The time-course and magnitude of fibre outgrowth from septal suspension grafts injected into the previously denervated hippocampal formation was monitored by measurements of choline acetyltransferase (ChAT), and the activity of the grafted neurons was assessed by measurements of [14C]acetylcholine (ACh) synthesis from [14C]glucose in vitro. Graft-derived ChAT activity was barely detectable 10 days after grafting, but increased sharply between 10 days and 1 month in the areas of the hippocampus located close to the septal implants. By 6 months ChAT activity was restored to near normal levels in all segments of the previously denervated hippocampus. The overall hippocampal [14C]ACh synthesis was also restored to normal levels in the grafted animals, and estimates of the ACh turnover rate suggested that the transmitter machinery of the newly established "septo-hippocampal" connections operated at a rate similar to that of the intrinsic septohippocampal pathway. The intrahippocampal septal suspension grafts, similar to the intrastriatal nigral grafts, thus seem to be capable of maintaining function at a relatively "physiological" level despite their abnormal positions.},
  author       = {Björklund, Anders and Gage, F H and Schmidt, R H and Stenevi, U and Dunnett, S B},
  keyword      = {Isogeneic
Acetylcholine
Choline O-Acetyltransferase,Acetylcholine/biosynthesis
Animals
Choline O-Acetyltransferase/metabolism*
Embryo,Inbred Strains
Septum Pellucidum/transplantation*
Time Factors
Transplantation,Mammalian/cytology
Female
Hippocampus/metabolism
Hippocampus/surgery
Neurons/metabolism
Neurons/transplantation*
Rats
Rats},
  language     = {eng},
  pages        = {59--66},
  publisher    = {ARRAY(0x8618dc8)},
  series       = {Acta Physiol.Scand.},
  title        = {Intracerebral grafting of neuronal cell suspensions. VII. Recovery of choline acetyltransferase activity and acetylcholine synthesis in the denervated hippccampus reinnervated by septal suspension implants},
  volume       = {Suppl. 522},
  year         = {1983},
}