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The crystal structure of the pathogenic collagen type II-specific mouse monoclonal antibody CIIC1 Fab: Structure to function analysis.

Uysal, Hüseyin LU ; Sehnert, Bettina; Nandakumar, Kutty S; Böiers, Ulrika; Burkhardt, Harald; Holmdahl, Rikard LU and Thunnissen, Marjolein M G M (2008) In Molecular Immunology 45. p.2196-2204
Abstract
Monoclonal anti-collagen type II antibody CIIC1 is an arthritogenic autoantibody, which induces arthritis in mice. We crystallized and solved the structure of CIIC1 Fab molecule. Analysis of structure revealed an interaction between the CDR regions of one Fab to the CH1 domain of another Fab, which resembles an antibody-antigen interaction. ELISA experiments confirmed the cross-reactivity of both the full CIIC1 antibody and a single chain Fv fragment to other anti-collagen antibodies which are of different isotypes and epitope specificity. The rheumatoid factor like reactivity of CIIC1 antibody together with its collagen type II specificity may explain the pathogenicity of this antibody.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Immunology
volume
45
pages
2196 - 2204
publisher
Pergamon
external identifiers
  • PMID:18241923
  • WOS:000254942400008
  • Scopus:40349084125
ISSN
1872-9142
DOI
10.1016/j.molimm.2007.12.005
language
English
LU publication?
yes
id
be04e088-6fbb-4aab-a9ab-1af1ecd6e5ee (old id 1042344)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18241923?dopt=Abstract
date added to LUP
2008-03-06 14:18:17
date last changed
2016-10-13 04:25:12
@misc{be04e088-6fbb-4aab-a9ab-1af1ecd6e5ee,
  abstract     = {Monoclonal anti-collagen type II antibody CIIC1 is an arthritogenic autoantibody, which induces arthritis in mice. We crystallized and solved the structure of CIIC1 Fab molecule. Analysis of structure revealed an interaction between the CDR regions of one Fab to the CH1 domain of another Fab, which resembles an antibody-antigen interaction. ELISA experiments confirmed the cross-reactivity of both the full CIIC1 antibody and a single chain Fv fragment to other anti-collagen antibodies which are of different isotypes and epitope specificity. The rheumatoid factor like reactivity of CIIC1 antibody together with its collagen type II specificity may explain the pathogenicity of this antibody.},
  author       = {Uysal, Hüseyin and Sehnert, Bettina and Nandakumar, Kutty S and Böiers, Ulrika and Burkhardt, Harald and Holmdahl, Rikard and Thunnissen, Marjolein M G M},
  issn         = {1872-9142},
  language     = {eng},
  pages        = {2196--2204},
  publisher    = {ARRAY(0xbc71b00)},
  series       = {Molecular Immunology},
  title        = {The crystal structure of the pathogenic collagen type II-specific mouse monoclonal antibody CIIC1 Fab: Structure to function analysis.},
  url          = {http://dx.doi.org/10.1016/j.molimm.2007.12.005},
  volume       = {45},
  year         = {2008},
}