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Expression of the transcription factor 7-like 2 gene (TCF7L2) in human adipocytes is down regulated by insulin.

Ahlzén, Maja; Johansson, Lovisa E; Cervin, Camilla LU ; Tornqvist, Hans; Groop, Leif LU and Ridderstråle, Martin LU (2008) In Biochemical and Biophysical Research Communications 370. p.49-52
Abstract
Variants in the TCF7L2 gene (transcription factor 7-like 2) have shown strong association with type 2 diabetes with two defined risk haplotypes, HapA and HapB(T2D). TCF7L2 may play a role in both glucose homeostasis and adipogenesis. Our aim was to characterize the TCF7L2 mRNA expression and regulation in human adipose tissue. We quantified TCF7L2 mRNA levels in cultured human adipocytes and in biopsies from visceral (VAT) and subcutaneous (SAT) adipose tissue from 38 obese non-diabetic subjects, using real-time PCR. The influence of haplotype and clinical traits on TCF7L2 mRNA levels were investigated. In vitro, insulin decreased TCF7L2 mRNA expression. This effect was attenuated in cells incubated with the free fatty acids palmitate or... (More)
Variants in the TCF7L2 gene (transcription factor 7-like 2) have shown strong association with type 2 diabetes with two defined risk haplotypes, HapA and HapB(T2D). TCF7L2 may play a role in both glucose homeostasis and adipogenesis. Our aim was to characterize the TCF7L2 mRNA expression and regulation in human adipose tissue. We quantified TCF7L2 mRNA levels in cultured human adipocytes and in biopsies from visceral (VAT) and subcutaneous (SAT) adipose tissue from 38 obese non-diabetic subjects, using real-time PCR. The influence of haplotype and clinical traits on TCF7L2 mRNA levels were investigated. In vitro, insulin decreased TCF7L2 mRNA expression. This effect was attenuated in cells incubated with the free fatty acids palmitate or oleate. In vivo, we found significantly higher expression in SAT from more insulin resistant subjects. No correlations between TCF7L2 mRNA expression and obesity measures were observed. TCF7L2 expression was higher in VAT than in SAT and when stratifying for haplotype, this difference was seen in HapA carriers but not in non-HapA carriers. In conclusion, TCF7L2 mRNA levels in adipocytes are decreased by insulin and seem to increase in insulin resistant subjects and in HapA carriers. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemical and Biophysical Research Communications
volume
370
pages
49 - 52
publisher
Elsevier
external identifiers
  • PMID:18342627
  • WOS:000255426700010
  • Scopus:41949129303
ISSN
1090-2104
DOI
10.1016/j.bbrc.2008.03.006
language
English
LU publication?
yes
id
7dc7701d-8989-4099-bd8b-1babbb40be98 (old id 1052456)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18342627?dopt=Abstract
date added to LUP
2008-04-01 08:24:52
date last changed
2016-10-13 04:36:38
@misc{7dc7701d-8989-4099-bd8b-1babbb40be98,
  abstract     = {Variants in the TCF7L2 gene (transcription factor 7-like 2) have shown strong association with type 2 diabetes with two defined risk haplotypes, HapA and HapB(T2D). TCF7L2 may play a role in both glucose homeostasis and adipogenesis. Our aim was to characterize the TCF7L2 mRNA expression and regulation in human adipose tissue. We quantified TCF7L2 mRNA levels in cultured human adipocytes and in biopsies from visceral (VAT) and subcutaneous (SAT) adipose tissue from 38 obese non-diabetic subjects, using real-time PCR. The influence of haplotype and clinical traits on TCF7L2 mRNA levels were investigated. In vitro, insulin decreased TCF7L2 mRNA expression. This effect was attenuated in cells incubated with the free fatty acids palmitate or oleate. In vivo, we found significantly higher expression in SAT from more insulin resistant subjects. No correlations between TCF7L2 mRNA expression and obesity measures were observed. TCF7L2 expression was higher in VAT than in SAT and when stratifying for haplotype, this difference was seen in HapA carriers but not in non-HapA carriers. In conclusion, TCF7L2 mRNA levels in adipocytes are decreased by insulin and seem to increase in insulin resistant subjects and in HapA carriers.},
  author       = {Ahlzén, Maja and Johansson, Lovisa E and Cervin, Camilla and Tornqvist, Hans and Groop, Leif and Ridderstråle, Martin},
  issn         = {1090-2104},
  language     = {eng},
  pages        = {49--52},
  publisher    = {ARRAY(0xb47aee8)},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Expression of the transcription factor 7-like 2 gene (TCF7L2) in human adipocytes is down regulated by insulin.},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2008.03.006},
  volume       = {370},
  year         = {2008},
}