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Cell and Particle Trapping in Microfluidic Systems Using Ultrasonic Standing Waves

Evander, Mikael LU (2008)
Abstract
Analysis methods are currently being miniaturized in order to save time and money while achieving higher

sensitivities. The ultimate goal is to create a lab-on-a-chip where all analysis steps and instruments can be

automated and integrated into a single chip. In order to perform cellassays and microparticle based

bioassays on chip, methods to manipulate particles and cells in microsystems are desired. This thesis

describes the development of a non-contact method of manipulating cells and particles in lab-on-a-chip

systems based on ultrasonic standing waves. A short review on microfluidics and acoustics is presented,

followed by an overview of other techniques for trapping particles and... (More)
Analysis methods are currently being miniaturized in order to save time and money while achieving higher

sensitivities. The ultimate goal is to create a lab-on-a-chip where all analysis steps and instruments can be

automated and integrated into a single chip. In order to perform cellassays and microparticle based

bioassays on chip, methods to manipulate particles and cells in microsystems are desired. This thesis

describes the development of a non-contact method of manipulating cells and particles in lab-on-a-chip

systems based on ultrasonic standing waves. A short review on microfluidics and acoustics is presented,

followed by an overview of other techniques for trapping particles and cells in microsystems. Previous work

done within the field of acoustic trapping in macro- and microsystems is reviewed before the development

and fabrication of the acoustic trapping platform is presented. The trapping platform provides a noncontact

way of immobilizing cells and particles in a continuously flowing microsystem. The possiblity to

use an array of trapping sites and move particles between different trapping sites is demonstrated. A model

bioassays is presented to show the potential of the dynamic arraying concept, where the combination of

microfluidics and an array of non-contact trapping sites is used to create a flexible platform for particlebased

assays. The platform is also shown to be a gentle way of immobilizing live cells as demonstrated by

culturing yeast cells suspended in a standing wave. A viability assay on levitated neural stem cells is also

performed to show handling of a more sensitive cell type. The technique is applied to the field of forensics

in sample preparation for DNA-analysis in rape cases. The acoustic technique is shown to achieve

comparable purities of the separated DNA fraction in a substantially shorter time as compared to the

standard methods used today. The results show that the acoustic trapping platform is a flexible and gentle

cell handling technique and has the potential to become an important tool for cell and particle handling in

microfluidic systems. Finally, an all-glass wet-etched device for acoustic continuous flow separations was

demonstrated. Previously reported devices have been manufactured in silicon and the possibility to use

glass as base material will lower the chip costs, simplifies the fabrication process and decrease the

fabrication time. (Less)
Please use this url to cite or link to this publication:
author
supervisor
opponent
  • Professor Dual, Jürg, Institute of Mechanical Systems, Swiss Federal Institute of Technology, Zürich, Switzerland
organization
publishing date
type
Thesis
publication status
published
subject
keywords
acoustic particle manipulation, standing waves, trapping, ultrasound, cell handling, particle handling, Microsystem technology, lab on a chip, microfluidics
pages
166 pages
publisher
Lund University
defense location
Room E:C, E-building, Ole Römers väg 3, Lund university, Faculty of Engineeringb
defense date
2008-06-05 13:15
ISBN
978-91-628-7525-1
language
English
LU publication?
yes
id
020d451d-ee80-4f4f-87ab-13f858c241e3 (old id 1149123)
date added to LUP
2008-05-09 15:36:08
date last changed
2016-09-19 08:45:14
@misc{020d451d-ee80-4f4f-87ab-13f858c241e3,
  abstract     = {Analysis methods are currently being miniaturized in order to save time and money while achieving higher<br/><br>
sensitivities. The ultimate goal is to create a lab-on-a-chip where all analysis steps and instruments can be<br/><br>
automated and integrated into a single chip. In order to perform cellassays and microparticle based<br/><br>
bioassays on chip, methods to manipulate particles and cells in microsystems are desired. This thesis<br/><br>
describes the development of a non-contact method of manipulating cells and particles in lab-on-a-chip<br/><br>
systems based on ultrasonic standing waves. A short review on microfluidics and acoustics is presented,<br/><br>
followed by an overview of other techniques for trapping particles and cells in microsystems. Previous work<br/><br>
done within the field of acoustic trapping in macro- and microsystems is reviewed before the development<br/><br>
and fabrication of the acoustic trapping platform is presented. The trapping platform provides a noncontact<br/><br>
way of immobilizing cells and particles in a continuously flowing microsystem. The possiblity to<br/><br>
use an array of trapping sites and move particles between different trapping sites is demonstrated. A model<br/><br>
bioassays is presented to show the potential of the dynamic arraying concept, where the combination of<br/><br>
microfluidics and an array of non-contact trapping sites is used to create a flexible platform for particlebased<br/><br>
assays. The platform is also shown to be a gentle way of immobilizing live cells as demonstrated by<br/><br>
culturing yeast cells suspended in a standing wave. A viability assay on levitated neural stem cells is also<br/><br>
performed to show handling of a more sensitive cell type. The technique is applied to the field of forensics<br/><br>
in sample preparation for DNA-analysis in rape cases. The acoustic technique is shown to achieve<br/><br>
comparable purities of the separated DNA fraction in a substantially shorter time as compared to the<br/><br>
standard methods used today. The results show that the acoustic trapping platform is a flexible and gentle<br/><br>
cell handling technique and has the potential to become an important tool for cell and particle handling in<br/><br>
microfluidic systems. Finally, an all-glass wet-etched device for acoustic continuous flow separations was<br/><br>
demonstrated. Previously reported devices have been manufactured in silicon and the possibility to use<br/><br>
glass as base material will lower the chip costs, simplifies the fabrication process and decrease the<br/><br>
fabrication time.},
  author       = {Evander, Mikael},
  isbn         = {978-91-628-7525-1},
  keyword      = {acoustic particle manipulation,standing waves,trapping,ultrasound,cell handling,particle handling,Microsystem technology,lab on a chip,microfluidics},
  language     = {eng},
  pages        = {166},
  publisher    = {ARRAY(0x81b4488)},
  title        = {Cell and Particle Trapping in Microfluidic Systems Using Ultrasonic Standing Waves},
  year         = {2008},
}