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Molecular correlates for maximal oxygen uptake (VO2max) and type 1 fibers.

Parikh, Hemang LU ; Nilsson, Emma LU ; Ling, Charlotte LU ; Poulsen, Pernille; Almgren, Peter LU ; Nittby, Henrietta LU ; Eriksson, Karl-Fredrik LU ; Vaag, Allan and Groop, Leif C (2008) In American Journal of Physiology: Endocrinology and Metabolism Apr 29. p.1152-1159
Abstract
Maximal oxygen uptake (VO2max) and the amount of type 1 fibers are interrelated but the underlying unifying molecular mechanisms are poorly understood. To explore these mechanisms we related gene expression profiles in skeletal muscle biopsies of 43 age-matched men from published datasets with VO2max and amount of type 1 fibers and replicated some of the findings in muscle biopsies from 154 young and elderly individuals using real-time PCR. We identified 66 probesets (genes or ESTs) positively and 83 probesets inversely correlated with VO2max and 171 probesets positively and 217 probesets inversely correlated with percentage of type 1 fibers in human skeletal muscle. Genes involved in oxidative phosphorylation (OXPHOS) showed high... (More)
Maximal oxygen uptake (VO2max) and the amount of type 1 fibers are interrelated but the underlying unifying molecular mechanisms are poorly understood. To explore these mechanisms we related gene expression profiles in skeletal muscle biopsies of 43 age-matched men from published datasets with VO2max and amount of type 1 fibers and replicated some of the findings in muscle biopsies from 154 young and elderly individuals using real-time PCR. We identified 66 probesets (genes or ESTs) positively and 83 probesets inversely correlated with VO2max and 171 probesets positively and 217 probesets inversely correlated with percentage of type 1 fibers in human skeletal muscle. Genes involved in oxidative phosphorylation (OXPHOS) showed high expression in individuals with high VO2max whereas the opposite was not the case in individuals with low VO2max. Instead, genes like AHNAK and BCL6 were associated with low VO2max. Also, expression of the OXPHOS genes NDUFB5 and ATP5C1 increased with exercise training and decreased with aging. In contrast, expression of AHNAK in skeletal muscle decreased with exercise training and increased with aging. Eleven genes (NDUFB4, COX5A, UQCRB, ATP5C1, ATP5G3, ETHE1, FABP3, ISCA1, MYST4, C9orf3 and PKIA) were positively correlated with both VO2max and percentage of type 1 fibers. VO2max closely reflects expression of OXPHOS genes, particularly of NDUFB5 and ATP5C1 in skeletal muscle suggesting good muscle fitness. In contrast, a high expression of AHNAK was associated with a low VO2max and poor muscle fitness. Key words: VO2max, Type 1 fibers, Aging. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
American Journal of Physiology: Endocrinology and Metabolism
volume
Apr 29
pages
1152 - 1159
publisher
American Physiological Society
external identifiers
  • WOS:000256469200017
  • PMID:18445752
  • Scopus:47549091170
ISSN
1522-1555
DOI
10.1152/ajpendo.90255.2008
language
English
LU publication?
yes
id
a3c6c8f6-1fbc-44e4-9e48-49839b830b22 (old id 1154675)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18445752?dopt=Abstract
date added to LUP
2008-06-04 13:16:31
date last changed
2016-10-13 04:29:08
@misc{a3c6c8f6-1fbc-44e4-9e48-49839b830b22,
  abstract     = {Maximal oxygen uptake (VO2max) and the amount of type 1 fibers are interrelated but the underlying unifying molecular mechanisms are poorly understood. To explore these mechanisms we related gene expression profiles in skeletal muscle biopsies of 43 age-matched men from published datasets with VO2max and amount of type 1 fibers and replicated some of the findings in muscle biopsies from 154 young and elderly individuals using real-time PCR. We identified 66 probesets (genes or ESTs) positively and 83 probesets inversely correlated with VO2max and 171 probesets positively and 217 probesets inversely correlated with percentage of type 1 fibers in human skeletal muscle. Genes involved in oxidative phosphorylation (OXPHOS) showed high expression in individuals with high VO2max whereas the opposite was not the case in individuals with low VO2max. Instead, genes like AHNAK and BCL6 were associated with low VO2max. Also, expression of the OXPHOS genes NDUFB5 and ATP5C1 increased with exercise training and decreased with aging. In contrast, expression of AHNAK in skeletal muscle decreased with exercise training and increased with aging. Eleven genes (NDUFB4, COX5A, UQCRB, ATP5C1, ATP5G3, ETHE1, FABP3, ISCA1, MYST4, C9orf3 and PKIA) were positively correlated with both VO2max and percentage of type 1 fibers. VO2max closely reflects expression of OXPHOS genes, particularly of NDUFB5 and ATP5C1 in skeletal muscle suggesting good muscle fitness. In contrast, a high expression of AHNAK was associated with a low VO2max and poor muscle fitness. Key words: VO2max, Type 1 fibers, Aging.},
  author       = {Parikh, Hemang and Nilsson, Emma and Ling, Charlotte and Poulsen, Pernille and Almgren, Peter and Nittby, Henrietta and Eriksson, Karl-Fredrik and Vaag, Allan and Groop, Leif C},
  issn         = {1522-1555},
  language     = {eng},
  pages        = {1152--1159},
  publisher    = {ARRAY(0xac67a90)},
  series       = {American Journal of Physiology: Endocrinology and Metabolism},
  title        = {Molecular correlates for maximal oxygen uptake (VO2max) and type 1 fibers.},
  url          = {http://dx.doi.org/10.1152/ajpendo.90255.2008},
  volume       = {Apr 29},
  year         = {2008},
}