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Exit of pediatric pre-B acute lymphoblastic leukaemia cells from the bone marrow to the peripheral blood is not associated with cell maturation or alterations in gene expression.

Hansson, Frida; Toporski, Jacek LU ; Månsson, Robert LU ; Johansson, Bertil LU ; Norén-Nyström, Ulrika; Jacobsen, Sten Eirik W LU ; Wiebe, Thomas LU ; Larsson, Marcus LU ; Sigvardsson, Mikael LU and Castor, Anders LU (2008) In Molecular Cancer 7.
Abstract
BACKGROUND: Childhood pre-B acute lymphoblastic leukemia (ALL) is a bone marrow (BM) derived disease, which often disseminates out of the BM cavity, where malignant cells to a variable degree can be found circulating in the peripheral blood (PB). Normal pre-B cells are absolutely dependent on BM stroma for survival and differentiation. It is not known whether transformed pre-B ALL cells retain any of this dependence, which possibly could impact on drug sensitivity or MRD measurements. RESULTS: Pre-B ALL cells, highly purified by a novel method using surface expression of CD19 and immunoglobulin light chains, from BM and PB show a very high degree of similarity in gene expression patterns, with differential expression of vascular... (More)
BACKGROUND: Childhood pre-B acute lymphoblastic leukemia (ALL) is a bone marrow (BM) derived disease, which often disseminates out of the BM cavity, where malignant cells to a variable degree can be found circulating in the peripheral blood (PB). Normal pre-B cells are absolutely dependent on BM stroma for survival and differentiation. It is not known whether transformed pre-B ALL cells retain any of this dependence, which possibly could impact on drug sensitivity or MRD measurements. RESULTS: Pre-B ALL cells, highly purified by a novel method using surface expression of CD19 and immunoglobulin light chains, from BM and PB show a very high degree of similarity in gene expression patterns, with differential expression of vascular endothelial growth factor (VEGF) as a notable exception. In addition, the cell sorting procedure revealed that in 2 out of five investigated patients, a significant fraction of the malignant cells had matured beyond the pre-B cell stage. CONCLUSION: The transition of ALL cells from the BM into the circulation does not demand, or result in, major changes of gene expression pattern. This might indicate an independence of BM stroma on the part of transformed pre-B cells, which contrasts with that of their normal counterparts. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Cancer
volume
7
publisher
BioMed Central
external identifiers
  • WOS:000258958600001
  • PMID:18694513
  • Scopus:50649098470
ISSN
1476-4598
DOI
10.1186/1476-4598-7-67
language
English
LU publication?
yes
id
77836718-7183-4458-b60d-17fd27bb8f17 (old id 1223287)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18694513?dopt=Abstract
date added to LUP
2008-09-02 13:51:40
date last changed
2016-10-13 04:35:36
@misc{77836718-7183-4458-b60d-17fd27bb8f17,
  abstract     = {BACKGROUND: Childhood pre-B acute lymphoblastic leukemia (ALL) is a bone marrow (BM) derived disease, which often disseminates out of the BM cavity, where malignant cells to a variable degree can be found circulating in the peripheral blood (PB). Normal pre-B cells are absolutely dependent on BM stroma for survival and differentiation. It is not known whether transformed pre-B ALL cells retain any of this dependence, which possibly could impact on drug sensitivity or MRD measurements. RESULTS: Pre-B ALL cells, highly purified by a novel method using surface expression of CD19 and immunoglobulin light chains, from BM and PB show a very high degree of similarity in gene expression patterns, with differential expression of vascular endothelial growth factor (VEGF) as a notable exception. In addition, the cell sorting procedure revealed that in 2 out of five investigated patients, a significant fraction of the malignant cells had matured beyond the pre-B cell stage. CONCLUSION: The transition of ALL cells from the BM into the circulation does not demand, or result in, major changes of gene expression pattern. This might indicate an independence of BM stroma on the part of transformed pre-B cells, which contrasts with that of their normal counterparts.},
  author       = {Hansson, Frida and Toporski, Jacek and Månsson, Robert and Johansson, Bertil and Norén-Nyström, Ulrika and Jacobsen, Sten Eirik W and Wiebe, Thomas and Larsson, Marcus and Sigvardsson, Mikael and Castor, Anders},
  issn         = {1476-4598},
  language     = {eng},
  publisher    = {ARRAY(0x95460a8)},
  series       = {Molecular Cancer},
  title        = {Exit of pediatric pre-B acute lymphoblastic leukaemia cells from the bone marrow to the peripheral blood is not associated with cell maturation or alterations in gene expression.},
  url          = {http://dx.doi.org/10.1186/1476-4598-7-67},
  volume       = {7},
  year         = {2008},
}