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Affinity Two-Phase Partitioning in Acoustically Levitated Drops

Santesson, Sabina LU ; Barinaga-Rementeria Ramírez, Irene LU ; Viberg, Peter LU ; Jergil, Bengt LU and Nilsson, Staffan LU (2004) In Analytical Chemistry 76(2). p.303-308
Abstract
Miniaturized (<1 L) biospecific affinity two-phase partitioning in an acoustically levitated drop is described. Miniaturization commonly gives unfavorable surface/volume ratios, but in the levitation approach adsorption problems are minimized since the only surrounding wall is the liquid/air interface of the drop. Biotinylated liposomes were partitioned in aqueous poly(ethylene glycol)/dextran two-phase drops with NeutrAvidin-dextran as the affinity ligand. A two-phase drop was trapped and manipulated in a node of a standing ultrasonic wave. Alternatively, a two-phase system was formed by levitation and evaporation of a polymer one-phase drop. Phase mixing was achieved by adjusting the ultrasonic field and phase separation by... (More)
Miniaturized (<1 L) biospecific affinity two-phase partitioning in an acoustically levitated drop is described. Miniaturization commonly gives unfavorable surface/volume ratios, but in the levitation approach adsorption problems are minimized since the only surrounding wall is the liquid/air interface of the drop. Biotinylated liposomes were partitioned in aqueous poly(ethylene glycol)/dextran two-phase drops with NeutrAvidin-dextran as the affinity ligand. A two-phase drop was trapped and manipulated in a node of a standing ultrasonic wave. Alternatively, a two-phase system was formed by levitation and evaporation of a polymer one-phase drop. Phase mixing was achieved by adjusting the ultrasonic field and phase separation by readjusting the field. NeutrAvidin-dextran brought about the redistribution of biotinylated liposomes from the poly(ethylene glycol)-rich phase into the dextran-rich phase. Thus, an entire affinity two-phase separation procedure, including mixing of the phases and incubation to allow affinity interactions to develop under constant volume, followed by phase separation under controlled evaporation, can be performed in a single levitated drop. This miniaturized technique would allow the separation of biologically active membranes or organelles from individual cells for analysis. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
76
issue
2
pages
303 - 308
publisher
The American Chemical Society
external identifiers
  • PMID:14719875
  • WOS:000188198800008
  • Scopus:0346459923
ISSN
1520-6882
DOI
10.1021/ac034951h
language
English
LU publication?
yes
id
a6b42434-befc-4044-8056-3158dc1c3420 (old id 126105)
date added to LUP
2007-06-27 15:18:33
date last changed
2016-12-04 04:45:28
@misc{a6b42434-befc-4044-8056-3158dc1c3420,
  abstract     = {Miniaturized (&lt;1 L) biospecific affinity two-phase partitioning in an acoustically levitated drop is described. Miniaturization commonly gives unfavorable surface/volume ratios, but in the levitation approach adsorption problems are minimized since the only surrounding wall is the liquid/air interface of the drop. Biotinylated liposomes were partitioned in aqueous poly(ethylene glycol)/dextran two-phase drops with NeutrAvidin-dextran as the affinity ligand. A two-phase drop was trapped and manipulated in a node of a standing ultrasonic wave. Alternatively, a two-phase system was formed by levitation and evaporation of a polymer one-phase drop. Phase mixing was achieved by adjusting the ultrasonic field and phase separation by readjusting the field. NeutrAvidin-dextran brought about the redistribution of biotinylated liposomes from the poly(ethylene glycol)-rich phase into the dextran-rich phase. Thus, an entire affinity two-phase separation procedure, including mixing of the phases and incubation to allow affinity interactions to develop under constant volume, followed by phase separation under controlled evaporation, can be performed in a single levitated drop. This miniaturized technique would allow the separation of biologically active membranes or organelles from individual cells for analysis.},
  author       = {Santesson, Sabina and Barinaga-Rementeria Ramírez, Irene and Viberg, Peter and Jergil, Bengt and Nilsson, Staffan},
  issn         = {1520-6882},
  language     = {eng},
  number       = {2},
  pages        = {303--308},
  publisher    = {ARRAY(0xa28a678)},
  series       = {Analytical Chemistry},
  title        = {Affinity Two-Phase Partitioning in Acoustically Levitated Drops},
  url          = {http://dx.doi.org/10.1021/ac034951h},
  volume       = {76},
  year         = {2004},
}