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Molecular characterization of invasive and non-invasive Streptococcus pyogenes isolates from Romania.

Luca, Bogdan LU ; Straut, Monica; Cretoiu, Silvia; Surdeanu, Maria; Ungureanu, Vasilica; van der Linden, Mark and Jasir, Aftab LU (2008) In Journal of Medical Microbiology 57(Pt 11). p.1354-1363
Abstract
In 2002, the Romanian National Reference Laboratory was invited to join the Strep-EURO project to study invasive Streptococcus pyogenes infections. During 2003 and 2004, a total of 33 isolates recovered from invasive disease were received from eight Romanian counties. For comparison, 102 isolates from non-invasive disease, as well as a collection of 12 old invasive strains (isolated between 1967 and 1980) were included. All isolates were characterized by several methods: T and emm typing, presence of the fibronectin-binding protein F1 gene (prtF1), serum opacity factor (sof), and superantigen (SAg) genes (speA, speB, speC, speF, speG, speH, ssa and smeZ). The recent invasive isolates exhibited 19 emm-types, of which emm1, emm81, emm76,... (More)
In 2002, the Romanian National Reference Laboratory was invited to join the Strep-EURO project to study invasive Streptococcus pyogenes infections. During 2003 and 2004, a total of 33 isolates recovered from invasive disease were received from eight Romanian counties. For comparison, 102 isolates from non-invasive disease, as well as a collection of 12 old invasive strains (isolated between 1967 and 1980) were included. All isolates were characterized by several methods: T and emm typing, presence of the fibronectin-binding protein F1 gene (prtF1), serum opacity factor (sof), and superantigen (SAg) genes (speA, speB, speC, speF, speG, speH, ssa and smeZ). The recent invasive isolates exhibited 19 emm-types, of which emm1, emm81, emm76, emm49 and emm78 covered 57 % of the strains. Furthermore, multilocus sequence typing analysis revealed nine new sequence types, corresponding to emm types 1, 12, 49, 81, 92, 100, 106 and 119. The non-invasive isolates comprised 24 different emm types with a predominance of emm1 and 12; the old invasive strains were of eight emm types, of which four were unique for this group. All isolates harboured speB and speF; smeZ was detected in all invasive strains, except for the emm49 and emm81 isolates. The majority of isolates from carriers, and patients with pharyngitis were prtF1 positive, most of these (14 strains) being emm12. High tetracycline resistance rates were noted among both invasive and control isolates (54 % and 35 %, respectively), whereas macrolide resistance rates were low (3 % and 5 %, respectively). Active and continuing surveillance is required to provide an accurate assessment of the disease burden and to provide epidemiological data on the character of isolates in Romania. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Medical Microbiology
volume
57
issue
Pt 11
pages
1354 - 1363
publisher
Lippincott Williams & Wilkins
external identifiers
  • WOS:000260684100007
  • PMID:18927412
  • Scopus:55549119251
ISSN
0022-2615
DOI
10.1099/jmm.0.2008/001875-0
language
English
LU publication?
yes
id
7fc1af81-9b22-460e-8aea-009db3b14f43 (old id 1262138)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18927412?dopt=Abstract
date added to LUP
2008-11-03 13:02:13
date last changed
2016-10-13 04:35:30
@misc{7fc1af81-9b22-460e-8aea-009db3b14f43,
  abstract     = {In 2002, the Romanian National Reference Laboratory was invited to join the Strep-EURO project to study invasive Streptococcus pyogenes infections. During 2003 and 2004, a total of 33 isolates recovered from invasive disease were received from eight Romanian counties. For comparison, 102 isolates from non-invasive disease, as well as a collection of 12 old invasive strains (isolated between 1967 and 1980) were included. All isolates were characterized by several methods: T and emm typing, presence of the fibronectin-binding protein F1 gene (prtF1), serum opacity factor (sof), and superantigen (SAg) genes (speA, speB, speC, speF, speG, speH, ssa and smeZ). The recent invasive isolates exhibited 19 emm-types, of which emm1, emm81, emm76, emm49 and emm78 covered 57 % of the strains. Furthermore, multilocus sequence typing analysis revealed nine new sequence types, corresponding to emm types 1, 12, 49, 81, 92, 100, 106 and 119. The non-invasive isolates comprised 24 different emm types with a predominance of emm1 and 12; the old invasive strains were of eight emm types, of which four were unique for this group. All isolates harboured speB and speF; smeZ was detected in all invasive strains, except for the emm49 and emm81 isolates. The majority of isolates from carriers, and patients with pharyngitis were prtF1 positive, most of these (14 strains) being emm12. High tetracycline resistance rates were noted among both invasive and control isolates (54 % and 35 %, respectively), whereas macrolide resistance rates were low (3 % and 5 %, respectively). Active and continuing surveillance is required to provide an accurate assessment of the disease burden and to provide epidemiological data on the character of isolates in Romania.},
  author       = {Luca, Bogdan and Straut, Monica and Cretoiu, Silvia and Surdeanu, Maria and Ungureanu, Vasilica and van der Linden, Mark and Jasir, Aftab},
  issn         = {0022-2615},
  language     = {eng},
  number       = {Pt 11},
  pages        = {1354--1363},
  publisher    = {ARRAY(0x79cccf8)},
  series       = {Journal of Medical Microbiology},
  title        = {Molecular characterization of invasive and non-invasive Streptococcus pyogenes isolates from Romania.},
  url          = {http://dx.doi.org/10.1099/jmm.0.2008/001875-0},
  volume       = {57},
  year         = {2008},
}