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Neisseria meningitidis expressing lgtB lipopolysaccharide targets DC-SIGN and modulates dendritic cell function

Steeghs, Liana; van Vliet, Sandra; Uronen-Hansson, Heli LU ; van Mourik, Andries; Engering, Anneke; Sanchez-Hernandez, Martha; Klein, Nigel; Callard, Robin; van Putten, Jos P M and van der Ley, Peter, et al. (2006) In Cellular microbiology 8(2). p.316-325
Abstract
Neisseria meningitidis lipopolysaccharide (LPS) has been identified as a major determinant of dendritic cell (DC) function. Here we report that one of a series of meningococcal mutants with defined truncations in the lacto-N-neotetraose outer core of the LPS exhibited unique strong adhesion and internalization properties towards DC. These properties were mediated by interaction of the GlcNAc(beta1-3)-Gal(beta1-4)-Glc-R oligosaccharide outer core of lgtB LPS with the dendritic-cell-specific ICAM-3 grabbing non-integrin (DC-SIGN) lectin receptor. Activation of DC-SIGN with this novel oligosaccharide ligand skewed T-cell responses driven by DC towards T helper type 1 activity. Thus, the use of lgtB LPS may provide a powerful instrument to... (More)
Neisseria meningitidis lipopolysaccharide (LPS) has been identified as a major determinant of dendritic cell (DC) function. Here we report that one of a series of meningococcal mutants with defined truncations in the lacto-N-neotetraose outer core of the LPS exhibited unique strong adhesion and internalization properties towards DC. These properties were mediated by interaction of the GlcNAc(beta1-3)-Gal(beta1-4)-Glc-R oligosaccharide outer core of lgtB LPS with the dendritic-cell-specific ICAM-3 grabbing non-integrin (DC-SIGN) lectin receptor. Activation of DC-SIGN with this novel oligosaccharide ligand skewed T-cell responses driven by DC towards T helper type 1 activity. Thus, the use of lgtB LPS may provide a powerful instrument to selectively induce the desired arm of the immune response and potentially increase vaccine efficacy. (Less)
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publishing date
type
Contribution to journal
publication status
published
subject
in
Cellular microbiology
volume
8
issue
2
pages
316 - 325
publisher
Wiley-Blackwell
external identifiers
  • WOS:000234667200013
  • Scopus:33644821162
DOI
10.1111/j.1462-5822.2005.00623.x
language
English
LU publication?
no
id
96c24b5f-4052-4960-9a3d-23d9a58d3fb1 (old id 1298383)
date added to LUP
2009-07-10 12:02:53
date last changed
2016-11-20 04:25:14
@misc{96c24b5f-4052-4960-9a3d-23d9a58d3fb1,
  abstract     = {Neisseria meningitidis lipopolysaccharide (LPS) has been identified as a major determinant of dendritic cell (DC) function. Here we report that one of a series of meningococcal mutants with defined truncations in the lacto-N-neotetraose outer core of the LPS exhibited unique strong adhesion and internalization properties towards DC. These properties were mediated by interaction of the GlcNAc(beta1-3)-Gal(beta1-4)-Glc-R oligosaccharide outer core of lgtB LPS with the dendritic-cell-specific ICAM-3 grabbing non-integrin (DC-SIGN) lectin receptor. Activation of DC-SIGN with this novel oligosaccharide ligand skewed T-cell responses driven by DC towards T helper type 1 activity. Thus, the use of lgtB LPS may provide a powerful instrument to selectively induce the desired arm of the immune response and potentially increase vaccine efficacy.},
  author       = {Steeghs, Liana and van Vliet, Sandra and Uronen-Hansson, Heli and van Mourik, Andries and Engering, Anneke and Sanchez-Hernandez, Martha and Klein, Nigel and Callard, Robin and van Putten, Jos P M and van der Ley, Peter and van Kooyk, Yvette and van de Winkel, Jan G J},
  language     = {eng},
  number       = {2},
  pages        = {316--325},
  publisher    = {ARRAY(0x83b02c8)},
  series       = {Cellular microbiology},
  title        = {Neisseria meningitidis expressing lgtB lipopolysaccharide targets DC-SIGN and modulates dendritic cell function},
  url          = {http://dx.doi.org/10.1111/j.1462-5822.2005.00623.x},
  volume       = {8},
  year         = {2006},
}