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Selective immunolesion of cholinergic neurons leads to long-term changes in 5-HT2A receptor levels in hippocampus and frontal cortex.

Severino, Maurizio LU ; Pedersen, AF; Trajkovska, V; Christensen, E; Lohals, R; Veng, LM; Knudsen, GM and Aznar, S (2007) In Neuroscience Letters 428(1). p.47-51
Abstract
Although loss of cholinergic neurons in the basal forebrain is considered a key initial feature in Alzheimer's disease (AD), changes in other transmitter systems, including serotonin and 5-HT2A receptors, are also associated with early AD. The aim of this study was to investigate whether elimination of the cholinergic neurons in the basal forebrain directly affects 5-HT2A receptor levels. For this purpose intraventricular injection of the selective immunotoxin 192 IgG-Saporin was given to rats in doses of either 2.5 or 5 μg. The rats were sacrificed after 1, 2, 4 and 20 weeks. 5-HT2A protein levels were determined by western techniques in frontal cortex and hippocampus. A significant 70% downregulation in frontal cortex and a 100%... (More)
Although loss of cholinergic neurons in the basal forebrain is considered a key initial feature in Alzheimer's disease (AD), changes in other transmitter systems, including serotonin and 5-HT2A receptors, are also associated with early AD. The aim of this study was to investigate whether elimination of the cholinergic neurons in the basal forebrain directly affects 5-HT2A receptor levels. For this purpose intraventricular injection of the selective immunotoxin 192 IgG-Saporin was given to rats in doses of either 2.5 or 5 μg. The rats were sacrificed after 1, 2, 4 and 20 weeks. 5-HT2A protein levels were determined by western techniques in frontal cortex and hippocampus. A significant 70% downregulation in frontal cortex and a 100% upregulation in hippocampus of 5-HT2A receptor levels were observed 20 weeks after the cholinergic lesion when using the highest dose of 192 IgG-Saporin. Our results show that cholinergic deafferentation leads to decreased frontal cortex and increased hippoca (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Neuroscience Letters
volume
428
issue
1
pages
47 - 51
publisher
Elsevier
external identifiers
  • Scopus:35448937166
ISSN
0304-3940
DOI
10.1016/j.neulet.2007.09.026
language
English
LU publication?
no
id
b56423ec-c87e-4e64-852c-5bde9806c212 (old id 1298974)
date added to LUP
2009-07-09 14:26:13
date last changed
2016-10-13 04:38:36
@misc{b56423ec-c87e-4e64-852c-5bde9806c212,
  abstract     = {Although loss of cholinergic neurons in the basal forebrain is considered a key initial feature in Alzheimer's disease (AD), changes in other transmitter systems, including serotonin and 5-HT2A receptors, are also associated with early AD. The aim of this study was to investigate whether elimination of the cholinergic neurons in the basal forebrain directly affects 5-HT2A receptor levels. For this purpose intraventricular injection of the selective immunotoxin 192 IgG-Saporin was given to rats in doses of either 2.5 or 5 μg. The rats were sacrificed after 1, 2, 4 and 20 weeks. 5-HT2A protein levels were determined by western techniques in frontal cortex and hippocampus. A significant 70% downregulation in frontal cortex and a 100% upregulation in hippocampus of 5-HT2A receptor levels were observed 20 weeks after the cholinergic lesion when using the highest dose of 192 IgG-Saporin. Our results show that cholinergic deafferentation leads to decreased frontal cortex and increased hippoca},
  author       = {Severino, Maurizio and Pedersen, AF and Trajkovska, V and Christensen, E and Lohals, R and Veng, LM and Knudsen, GM and Aznar, S},
  issn         = {0304-3940},
  language     = {eng},
  number       = {1},
  pages        = {47--51},
  publisher    = {ARRAY(0x718c178)},
  series       = {Neuroscience Letters},
  title        = {Selective immunolesion of cholinergic neurons leads to long-term changes in 5-HT2A receptor levels in hippocampus and frontal cortex.},
  url          = {http://dx.doi.org/10.1016/j.neulet.2007.09.026},
  volume       = {428},
  year         = {2007},
}